Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIV, Issue 2
Written by Ruxandra TUDOR, Andra DEGAN, Ruxandra COSTEA, Gabriel PREDOI

This study was performed in order to evaluate the postoperative analgesic management of geriatric dogs during the first 12 hours after the soft tissue surgery. The study was conducted on sixty dogs, aged between 8-16 years, that were presented at the Faculty of Veterinary Medicine of Bucharest for soft tissue surgery procedures, between August - November 2017. Patients were divided in four groups (15 dogs /group) assigned to a different analgesic management that we intended to evaluate (Group 1- Tramadol, Group 2- Lidocaine in constant rate infusion, Group 3- Acetaminophen, Group 4- Ketamine+Lidocaine). Premedication was made with Midazolam 0.2 mg/kg and Butorphanol 0.2 mg/kg, injected intramuscularly (IM). Induction was obtained with Propofol 4-6 mg/kg intravenously. All patients were intubated, and maintenance was performed with Isoflurane. All patients were evaluated after the procedures using the Glasgow Composite Pain Scale (GCPS). During the evaluation period, the best results were recorded in groups 2 and 3 which were given Lidocaine and Lidocaine+Ketamine in a constant rate infusion with a pain scale of 2/24, compared with lower results in groups 1 and 4 with a GCPS of 5/24, where additional analgesic medication was needed.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIII, Issue 1
Written by Dan Alexandru ENACHE, Stelian BARAITAREANU, Marius DAN, Maria Rodica GURAU, Fanel OTELEA, Armenac DOBRE, Doina DANES

Maedi-Visna (MV) and Caprine Arthritis Encephalitis (CAE) are diseases of sheep and goats. They are caused by lentiviruses which belong to Retroviridae family. The usual way of contamination is the cohabitation of animals. Diseases are widespread in many countries as: Norway, France, Italy, Spain, USA, Panama, Cyprus, Greece, and Japan. The present paper aimed to present the MVV and CAEV antibodies seroprevalence in samples collected in different sheep and goats farms from Romania. There were collected blood samples from the following counties: Cluj-Napoca, Ilfov, Constanta, Galati, Giurgiu, Braila, Arges, Bacau, Dâmbovita, Ialomita, Suceava, Calarasi, Buzau, Vrancea, and Vaslui. In order to determine the presence of antibodies, the samples were analysed by indirect ELISA, using commercial kits. There were registered negative results in only four counties and the possible existence of viruses in farms cannot be excluded. In order to confirm and strengthen the preliminary results, we recommend to analyses the samples by molecular biology techniques. Also, national authorities could establish a program of surveillance and diagnosis at national level, able to provide a more complete picture of the SRLVs prevalence in each county.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1
Written by Nue MARKU, Kristaq BËRXHOLI, Jeton SPAHIU, Kurtesh SHERIFI, Agim REXHEPI

In recent years animal viral diseases transmitted by vectors are distributed fairly quickly in different regions of the world, including bluetongue virus (BTV). BT is a disease of ruminants transmitted by midges of the species Culicoides. The objective of the study was to describe seroprevalence rate of bluetounge virus in sheep flocks in different regions in Kosovo. The blood samples were collected randomly from sheep herds. The total 355 samples were collected, in 55 sheep herds, from 9 municipalities in 42 villages in whole Kosovo, between year 2014 and 2015. Antibodies for BTV in sera were detected by competitive enzyme-linked immunosorbent assay (c-ELISA) according to manufacturer instructions. Out of total 355 serum samples 32 sheep (9.014) were positive for BTV antibodies, from 9 municipalities in two regions. The highest prevalence of BTV antibodies were detected in Vitia municipality (16.42%) followed by Kamenica (14.81%), and Gjilani (10.00%), municipalities belonging to the same region, and the lowest prevalence in Shterpc (5.56), and Kaçanik (5.26%). This study describes seroprevalence of BTV in sheep flocks in Kosovo indicating widespread prevalence of BTV antibodies in studied regions.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Kalin HRISTOV, Parvan PARVANOV, Roman PEPOVICH, Branimir NIKOLOV

The purpose of this study was to investigate the prevalence of mastitis and dynamics of different mastitis forms in goats during lactation and the dry period .The prevalence of mastitis was analyzed in 250 goats. Parallel traced 52 dairy halves with different mastitis forms during lactation and 56 halves during the dry period. Express diagnosis was made on the farm with rapid mastitis test CMT-Test (Kruuse, Denmark) and test Porta SCC (Porta Check, USA). For precise determination of the health condition of all dairy halves was conducted laboratory analysis including determining the number of somatic cells by Fossomatic (Foss, Denmark) and microbiological testing for isolation of pathogenic microorganisms. Prevalence of mastitis was found within 45.8 % and results indicated that 41.67 % of the cases diagnosed with subclinical mastitis in early lactation, persisted at the end of the lactation period. At the same time the latent infection persisted in 15.38 % while secretory disorder was in 26.67 %. During the dry period the highest percentage of persisting indicated subclinical mastitis - 71.43 % and only 14.29 % were found healing, compared with secretory disorder that persisted in 42.86 % as they were healed halves. The latent infection persisted also in 42.86 %, but healing again was found only in 14.29 %. Non-clinical mastitis in the absence of the treatment are stored in 76.19%

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 2
Written by Aleksandar STANOJKOVIC, Dusica OSTOJIC-ANDRIC, Milica PETROVIC, Nikola STANISIC, Marija GOGIC, Aleksandra STANOJKOVIC-SEBIC, Cedomir RADOVIC

The goal of this paper is to present the prevalence of major disease causing sertotype 2 of Streptococcus suis in meat ready for retail market shipment. Streptococcus suis is one of the most important pig pathogen causing septicemia, meningitis and other infections in affected animals. In addition this bacteria is an emerging zoonotic pathogen. Human infections are usually after close contact with pigs or their products. A total of 180 samples of raw pork meat (200 gr each) were taken at sloughterhouse. Samples were taken from different parts of pork, already prepared for market: liver, kidneys, shoulder, ham, loin, belly, and head area. Results have shown that 18 isolates were identified as Streptococcus suis serotype 2 from 180 samples examined. The rate of prevalence was 10% exactly. Serotype 2 was the most isolated serotype from fresh pork with 46,1 % of isolated S. suis serotypes followed by serotype 9, 7, 3, 1 and 4. Streptococcus suis serotype 2 had been isolated in all collected samples. Prevalence of serotype 2 in liver, kidneys, shoulder, ham, loin, belly and head was 20%, 12%, 5%, 5%, 5%, 10% and 25 % respectively. It is known that besides occupational exposure and meat processing in sloughterhouses, consuming of uncooked or partially cooked pork products is also a risk factor for infection. Therfore it should be advised that pork need to be thoroughly cooked/baked and not to be in contact with raw meat in any way.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Vili Robert VOICHIŢOIU, János DÉGI, Viorel HERMAN

This study was conducted to investigate the prevalence of zoonotic staphylococci isolated from dogs in Western Romania. Samples were obtained from adult dogs of both sexes submitted to the University Veterinary Clinics Timisoara and private veterinary practice. Animals selected for this study had no known history of previous antibiotic treatment. Samples were identified and labeled as to source, male or female, adult and the anatomical area of harvesting. A total of 51 samples were obtained from different anatomical sites such as nose, eyes, ears, extremities reproductive and skin. After growth, staphylococcal isolates were identied according to their characteristics as outlined in Bergey’s Manual of Determinative Bacteriology and the Manual of Clinical Microbiology. 35 samples were positive for staphylococci, being isolated both positive and coagulase-negative species. The species most frequently isolated were S. (pseud)intermedius, S. aureus, S. hycus, S. epidermidis and S. haemolyticus. The prevalence of staphylococcal infections in veterinary medicine is increasing worldwide. Staphylococci have shown a frequent and rapid development of nosocomial infections. Unfortunately, these studies have not been documented continuously in veterinary medicine. The present investigation has examined the clinical prevalence of zoonotic staphylococci in the dogs that may constitute a reservoir for these bacteria.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Dan CRÎNGANU, Rǎzvan NEGREANU, Raluca NEGREANU

Lymphomas are malignant monoclonal cell proliferation having the starting point in the organized lymphoid tissue (spleen, thymus, limfocentrii) or the diffused lymphoid tissue (gut-Payer boards and tonsils) The different types of lymphomas are common regarding the symptoms, but differs depending on changes that occur at the cellular level and proliferating cell type, proliferation type and how they echo through out the body. Age and sex of the animals have an importan role because this form of cancer is identified by the age of 2 (years) with maximum frequency between 5 and 7 years old, but also with cases at age 10 in both sexes. Breeds that are most often affected are: Boxer, Scottish Terrier, Airedale Terrier, German Shepherd, Poodle, Rottweiler and Golden Retriver. Purebred dogs are more sensitive than half breeds. The color black appears to be a factor due to absorption of ultraviolet radiation, dogs with dark fur being exposed to both skin tumors and NHL. Lymphoma diagnosis begins with clinical examination revealing enlarged lymph nodes and sometimes the presence of general signs of illness: fever, cluster, weight loss, dyspnea, anorexia, anemia. Lymphosarcoma classification is based on location, histological criteria and immunophenotypic features.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Cristina-Elena DINU-PÎRVU, Mihaela Violeta GHICA, Alina ORTAN, Alexandru Nicolae POPESCU, Letitia PURDOIU, Lacramioara POPA

The aim of this study was the design of some topical drug systems such as hydrogels incorporating ibuprofen as a nonsteroidal antiinflammatory drug, having improved drug release characteristics and adequate rheological profiles, allowing a suitable spreading at the cutaneous administration of these formulations. The ibuprofen release profiles were obtained and analysed for the designed hydrogels, compared with the commercial Ibuprofen 5% (w/w) from Urgo. The kinetic experiments recommended the hydrogel containing the same weight of drug (ibuprofen 5% w/w) with the ingredients hydroxyethyl cellulose and glycerol, with a superior value for the diffusion coefficient and drug percentage released. The rheological studies confirmed the influence of formulation characteristics upon the primary biopharmaceutical profiles for the tested ibuprofen hydrogels.

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Paper presents the progress of the project VETERINARY EMERGENCY MEDICINE – INNOVATION AND NEW SKILLS IN HIGHER VETERINARY EDUCATION SYSTEM, co-financed by European Social Fund through the Human Resources Development Sectoral Operational Programme 2007-2013, implemented by the University of Agronomical Sciences and Veterinary Medicine Bucharest, University of Agricultural Sciences and Veterinary Medicine Cluj Napoca. The project aims to improve curriculum in higher veterinary education for a number of 400 students, in accordance with CNCIS, by introducing new disciplines and by developing a Veterinary Sectorial Network in order to achieve a better correlation between labor market and higher education system. Relevant information about the project can be found at the address: www.muv.fmvb.ro .

Paper presents the progress of the project IMPROVING QUALITY OF NATIONAL HIGHER EDUCATION SYSTEM IN ACCORDANCE WITH CHANGING KNOWLEDGE-BASED SOCIETY AND LABOR MARKET DYNAMICS, cofinanced by European Social Fund through the Human Resources Development Sectoral Operational Programme 2007-2013, implemented by the Ministry of Education, University of Agronomical Sciences and Veterinary Medicine Bucharest, University Politehnica Bucharest, University of Agricultural Sciences and Veterinary Medicine Cluj Napoca, University Politehnica Timisoara. The project aims to reorganize and improve the national higher educational system by developing an implementing an integrated approach - National Higher Education Consortium and quality management by increasing access to higher education for a number of 1000 students and by delivering training programs to 500 key actors involved in university processes and activities. Relevant information about the project can be found at the address: www.proiect-impact.ro .