Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Daniel Constantin Lescai
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Scientific Works Series C. Veterinary Medicine
PRINT ISSN 2065-1295, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Dégi János, Kálmán Imre, Viorel Herman, Cătană Nicolae
Blister disease is a common condition in reptiles due to poor environmental management – that is, housing the reptile in overly-moist or dirty surroundings. It is also known as vesicular dermatitis. Later, these scales become swollen and infected by opportunistic bacteria (Pseudomonas spp.). Small reptiles or those with weakened immune system (either from previous illness, malnutrition or stress) can go downhill rapidly and die very fast from blister disease. This report describes a diagnostic and treatment strategy for an infectious dermatitis in a boa (Boa constrictor).
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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Alina Anton, Gheorghe Solcan
Adrenocorticotropic hormone (ACTH) challenge test is recognized as a method for evaluating some forms of stress. Six lacting cows, well trained to blood sampling were used for this study. Cows were randomly assigned to receive saline or an intramuscular single dose (0.5 μg/kg) of ACTH (Synacthen Depot). Blood samples (10 mL) were collected from the coccygeal blood vessels of all cows at 0 h (immediately before treatment) and every 30 min for 2 h to measure serum cortisol, glucose, creatinine and urea concentrations. Each blood collection included a separate puncture of the coccygeal blood vessels using a new needle. Respiratory frequency was measured for each cow at 0, 30, 60, 90 and 120 min. Serum cortisol concentrations of cows did not differ between treatments at the initiation of treatments; however, serum cortisol, glucose, creatinine, urea concentrations and respiratory frequency were affected by ACTH, time, and the interaction of ACTH x time. Administration of ACTH increased (P < 0.05) serum cortisol concentration in cows within 30 min of administration, and concentrations remained increased throught the blood sampling period. Cows that received ACTH had increased (P < 0.05) respiratory frequency within 30 min of administration. An increase in hypothalamic pituitary-adrenocortical activity, causes the rise of blood cortisol, indicates a physiological response to different stressors.
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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Oana-Mărgărita Ghimpeţeanu, Cristina Ţoca), George-Bogdănel Roşu), Elena Mitrănescu, Florica Bărbuceanu, Manuella Militaru
Poultry liver is considered to be one of the most important sources of mineral nutrients in humans' diet, but due to its specific structure tends to bind chemical contaminants such as heavy metals and pesticides. The aim of this study was to perform a short characterization of mineral nutrients concentration and heavy metals and pesticides contamination in commercial poultry liver samples and their possible effect on food safety. Eleven commercial poultry liver samples coming from 3 different Romanian slaughterhouses were submitted to analysis. Heavy metals (Cd, Pb, Al) and mineral nutrients (Ni, Cr, Mn, Cu, Fe, Zn, Ca, Mg, K, Na) were determined by Inductively Coupled Plasma-Mass Spectrometry. For organochlorine and organophosphorus pesticides quantification Gas Chromatography/Mass Spectrometry was used. The concentrations for heavy metals ranged from 0.008 to 0.03 mg/kg Cd, 0.02 to 0.06 mg/kg Pb, 0.001 to 0.002 mg/kg Hg and 0.09 to 0.6 mg/kg Al. For all samples, the values of organochlorine and organophosphorus pesticides were under the limit of detection. For mineral nutrients, concentrations ranged from 0.02 to 0.07 mg/kg Ni; 0.02 to 0.09 mg/kg Cr; 0.5 to 1.32 mg/kg Mn; 0.9 to 2.6 mg/kg Cu; 12.9 to 48.7 mg/kg Fe; 3.7 to 7.7 mg/kg Zn, 35.6 to 62.3 mg/kg Ca, 86.6 to 167.2 mg/kg Mg, 1555.9 to 1668 mg/kg K, 593.51 to 1127.8 mg/kg Na. Although it is known that people ingest heavy metals and pesticides from animal products, the concentrations obtained in this study showed that there is no risk for human health linked to the consumption of poultry liver.
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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Bradea A, Codreanu M.D., Vlagioiu C.
Kidneys are organs that receive a large amount of blood, approximately 20% of the heart blood flow. Many renal diseases have an important vascular component, some systemic diseases, such as hypertension, are vascularly mediated through the juxtaglomerular component. The study has been carried out on a number of 25 cats and 18 dogs, inside the Faculty of Veterinary Medicine Bucharest, sedated and awake as well as with different renal, hepatic and heart diseases. The resistance index and pulsatility index have been appreciated in renal level from the renal arteries, interlobar arteries and arcuate arteries, with superior specificity in pathologic conditions (diagnosis and prognosis)
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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by S.A. Pașca, Gh. Solcan, E.V. Șindilar, M. Lazăr
Anti-freeze intoxication is most frequently encountered in dogs and cats after accidental consumption of the liquid emptied from car radiators. In ruminants, the intoxication can appear as a consequence of erratic contamination of grazing fields with the liquid from tractor tires. Other cases have been reported, due to erronate treatments applied to silage, when ethilenglicole is mistaken taken for formic acid, or after contaminated water consumption. Ethylen glycol is oxidized by alcohol dehydrogenase in the liver to glycoaldehide, wich is in turn oxidized to glycolic acid, glyoxalate, and finally, oxalate. Calcium oxalates crystals may be found in tubular lamina, tubular cells and the interstitium; they are light yellow, arranged in rosettes or prisms, and are birefringent in polarized light. Tubular lesions range from fat degeneration to necrosis. Large numbers of crystals in tubules are pathognomonic for ethylene glycol poisoning.
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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by CLINICAL, ULTRASOUND AND LABORATORY CHANGES IN CUSHING SYNDROME IN DOGS
Dogs` adrenal pathology is dominated by Cushing syndrome, mostly by iatrogenic origin or neoplasic glandular lesions. Clinical evaluation were performed following the classic screening protocol and additional hematological and biochemical investigations (liver/kidneys function), hormonal determinations (basal cortisol or after stimulation tests, i.e. suppression) and ultrasound exams. In our study were included 18 dogs with Cushing syndrome (hyperadrenocorticism). From clinical point of view the main registered clinical signs were bulimia, polyuria-polydipsia syndrome, abdominal ptosis, hepatomegaly, calcinosis and/or cutaneous hyper pigmentation, bilateral symmetrical alopecia). The biochemical blood profile registered changes (increased ALT and AST activity, hyperlipidemia, decreased serum urea levels, hyperglycemia) and urinary (diluted urine, proteinuria). Ultrasound reveals in case of affected adrenal glands appears as distinct structures, flattened shape, appearance lobe, located cranio-medial kidney, caudal of the mesenteric and celiac artery and cranial of the renal artery and the right (lower) prior to renal vein and cranial right kidney. According to their topography, size and structure the ultrasound changes were very useful for the diagnosis of the diseases related to adrenomegaly and changing their echogenicity and echostructure.
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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Bogdan Băcescu, Tănase Petruţ
The study was conducted on a total of 117 White New Zeeland breed rabbits, which were identified by feces examination with varying degrees of infestations with Eimeria sp., Eimeriosis hepatic lesions being identified in 20 of them. Specific lesions were hepatic hypertrophy with presence of necrotic miliary nodular centres, vesicular looking angiocolitis, apostematous hepatitis and cirrhosis. Histopathological examination pursued in particular the consequences of sexual ongoing phase. Biliary ducts were dilated with hyperplastic epithelial reaction and the formation of papillary reactions with the presence of asexual and sexual stages of development.
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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by B. Al. Viţălaru, D. Crânganu, G. Polter
In Romania, monochemotherapy using exclusively Vinca Rosea alkaloids created mutant cellular clones of Sticker sarcoma, activating MDR severe mutant genes. Materials and Methods: During this study, a number of 10 dogs with TVT, of different breeds, genders and ages have been studied. Blood tests, X-Rays, coagulation profile, biochemistry of the blood, urine dipstick, abdominal ultrasound and cytology - FNA from the biopsy mass were performed. All the submitted samples were analyzed in reference laboratories from both Romania and Netherlands. The history, clinical and histological findings were all compatible with TVT. The approach was different in the two Clinics. In UK, the therapeutic approach was different, using Vincristine 0,7mg/m2 week one, repeated every 7 days three more times. In Romania, monochemotherapy created mutant cellular clones of Sticker sarcoma. Therefore, polychemotherapy has been used (genital localization, expansive and proliferate pattern with no metastases). Preoperative, neoadjuvant polychemotherapy for cytoreduction, based on ciclophosphamide 50mg/m2 or ifosfamide 200mg/m2, cyclo dependent cytostatics, and 5-fluorouracil as an antimetabolite, 50mg/m2 and Vincristine 0,7mg/m2 week one, repeated every 14 days or after surgery to prevent recurrence. Results: All cases treated in UK with Vincristine and all Romanian dogs treated with polychemotherapy shown remission of the penile masses and complete healing. In Romania, monochemotherapy created mutant cellular clones. Conclusion: Numerous cases of TVT in the free dog population in Romania and uncontrolled breeding, along with the absence of neutering (castration) favored the spread of tumors and the transmission of resistance from one dog to another.
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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Mateescu Romaniţa, Tudor N., Mateescu C. Vlăgioiu C.
The current study presents current data on the evolution and prevalence of pancreatitis in dogs examined in the Agervet Târgovişte Clinic, Dâmboviţa County. Out of the 573 dogs with digestive disorders, 56 (9.77%) were diagnosed with pancreatitis. Twenty-one patients were male and 35 were female patients, aged 5 to 17 years. The clinical picture was polymorphic, and blood examination showed alterations in biochemical parameters. Based on the history of the clinical data, combined with additional examination data we determined that 17 (30.36%) had acute forms, and 39 (69.64%) had chronic forms. For the diagnosis rapid tests were used to assess the level of the specific pancreatic lipase. Also, histopathological examination was performed. Fibrosis, acinar atrophy and lymphocytic infiltration indicated the chronic form, while the pancreatic cell necrosis and neutrophilic infiltration indicated the acute form.
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