Scientific Works Series C. Veterinary Medicine

PRINT ISSN 2065-1295, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Catalina Posea, A. Șonea, A. Bîrțoiu, Monica Roman, Mihaela Vasile, Camelia Ion

Species of fungi: Aspergillus and Penicillium can produce and release, in certain circumstances of temperature, pH and humidity, secondary metabolites in fooders and food products type: Ochratoxins . Toxicity of Ochratoxin depens on the type (A,B,C), source and their dose, duration of exposure . Up to the present time has been demonstrated nephrotoxic compounds, hepatotoxic and teratogenic effect. of OchratoxinA (OTA) Due to the toxic effect of OTA the maximum level in fooder and food is subject to European legislation (Reg. CE N0 576/2006, Reg. CE 1881/2006) The aim of the paper is to highlight the value of mycotoxins type OTA in feed and foods, as an possible risk on animals and human health. The working method used for teting was ELISA. Values obtained from determinations were performed according to the legislation.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Cenariu M., Groza I., Pall Emoke, Parlapan Laura, Ilea Cristina

Embryo biopsy performed in order to obtain a small amount of blastomeres needed for embryo sexing is an invasive method that damages the zona pellucida and therefore decreases the survival capacity of the embryo that is subsequently submitted to cryopreservation. The aim of the present study was to evaluate three biopsy techniques applied in bovine embryos according to their capacity to maintain embryo viability after cryopreservation. Three embryo biopsy techniques (needle, blade and aspiration) were applied on 120 bovine embryos divided into 3 batches (n=40) in order to harvest the blastomeres needed for polymerase chain reaction (PCR) embryo sexing. After the biopsy, the embryos were frozen/thawed using the one step method with ethylene glycol and then transferred into synchronized recipients. DNA was extracted from blastomeres and amplified using bovine Y-chromosome specific primers, in order to determine the sex of the embryo. The pregnancy diagnosis and the assessment of pregnancy rate were performed 30 days after transfer using an ultrasound scanner. There was a significant difference in pregnancy rates according to the biopsy method used: 55% for the needle biopsy, 45% for the aspiration method and 30% for the microblade technique. The accuracy of the PCR sexing method was comparable in all batches, and therefore was not influenced by the biopsy method. The needle method of embryo biopsy proved to be the most suitable as it yielded the highest pregnancy rates and can be successfully applied when harvesting blastomeres for embryo sexing.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Tudor Poliana

Neotrombicula autumnalis is a mite belonging to Trombiculidae family, which lives free on soil and vegetation, and in larval stages becomes an ectoparasite, attacking wild and domestic animals and humans. The harvest mites are responsible for producing cutaneous pruriginous lesions in infested hosts. This study presents a trombidiosis case during winter season, in a 5 year old mongrel dog from the Eastern part of Romania. The dog presented a papulo-erythematous dermatitis, pruriginous, localized in interdigital spaces of posterior limbs area. Diagnosis has been established by microscopic identification of N. autumnalis parasitic larval stage from skin scraped material. To our knowledge this is the first report of trombidiosis in dog from the Eastern part of Romania, with evolution during winter season.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Gelu Zegrean

Monitoring the blood parameters in dogs undergoing ureteroenterostomy surgery reveal major changes in their values. Two weeks after surgery, creatinine, hemoglobin and total protein levels are within normal limits in case 1 and 2. In exchange, urea remains high compared to normal values even two weeks after the surgery.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by G.F. Tobă, A.T. Bogdan, M. Th. Paraschivescu, M.Cornilă, L. Ioniță , L.G. Tobă F.Bănățeanu

In Romania there are populations of endangered cattle as Grey Steppe breed and Pinzgau of Transylvania and Romanian Buffaloes number is a marked decline. An important role in preserving the "ex situ" is biotechnology breeding populations vulnerable to conserve animal genetic information but not breeding material: gametes usually stored sperm as semen coming from a single parent (haploid) and embryos with 2 parents (diploid) population structure that allows preservation by freezing. Material and method MOET program implementation (Multiple Ovulation Embryo Transfer) to 4 gray cattle breed cow, repeated ovarian stimulation with FSH and PMSG intervals: 55, 60, and 115 and 157 days, and at 2 Holstein Friesian cattle every: 52 and 87 days. Results Following these repeated treatments Grey Steppe breed cows resulted in 99 of the corpus luteum (11CL/DR) from or taken Embryonic Formations 69 (7.66 FE / DR), of which 40 were embryos transferred and of these, 27 were frozen and stored in liquid nitrogen at - 196 ° C. Following repeated application poliovulations FSH treatment every 52 to 87 days, 2 heifers HF, ovaries responded with a total of 31 CL 6.2-CL / DR to who prelevat12 FE and 2.4 FE / DR and 12 embryos were frozen blastocyst. FSH were poliovulations with a 4 cows and 6 Pinzgau of Transylvania Romanian buffaloes, a total of 10 female donors who have repeatedly poliovulations treatment, and the average number was the 8.4 CL / D, 1 CL lower than the average female with repeated poliovulations, the average number was 3.3 EFF EFF / D 3 also less than 6.7 EFF in the group of females with repeat poliovulations treatment. Conclusion We conclude that after several repeated hormone treatments poliovulations 6 embryo donor females found that health in general and particularly genital tract not suffer in comparison with the group of 10 females poliovulations once and morphology embryos was very good, allowing freezing and storage in gene banks

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by tutunaru alexandru cosmin , leau florin, şonea alexandru, sandersen charlotte

Alfaxalone is a neurosteroid with general anesthetic properties. His steroid structure, analog with progesterone, might have an influence on patient glycaemia. The aim of our study was to assess variations of glycaemia after alfaxalone induction. For this study we used 7 female adult laboratory rabbits premedicated with fentanyl (0.0125 mg/kg) and droperidol (0.625 mg/kg) intramuscular. Alfaxan® was administered intravenously at a total dose of 3mg/kg by a constant rate infusion over 60 seconds using a syringe pump. Blood samples were drawn prior to and at 2, 4, 6, 10, 15, 20, 30 after induction by an indwelling catheter in the central auricular artery for instant blood glucose determination.Mean value of arterial blood glucose recorded was 154,36 mg/dl (81-262) with the highest peak at minute 4 after induction. Glucose variation remained within normal limits with the highest value at minute 4. Other studies showed an increased in plasmatic glucose after progesterone administration. Women using the progesterone-T intrauterine device showed blood glucose increased after three hours. A study made on rats showed an increase of blood glucose 30 minutes after progesterone administration. The theory that alfaxalone may influence plasmatic glucose have to be further studied on a higher number of animals, however is interesting the fact that in minute four after induction when rabbits suffered the most profound cardio-respiratory depression, blood glucose was at the peak. More extensive monitoring and a pharmacokinetic study are needed to comment accurately on alfaxalone effects on rabbits.

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