Scientific Works Series C. Veterinary Medicine

PRINT ISSN 2065-1295, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LIX (1)
Written by Niculae TUDOR, Constantin VLĂGIOIU, Adrian COMÂRZAN, Adriana ALISTAR

The lameness associated with pain localized within acropodial region is common in horses, which enforces the use of radiological equipment in tracking changes in bone level as well as in adjacent soft tissues level. The most common acropodial diseases are represented by traumatic processes, followed by degenerative and infectious, which can evolve acute or chronic. The purpose of this study was describing semiological aspects noticed in radiological examination of acropodial region in horses. Radiographic images from animals with acropodial diseases were selected and examined inside the radiology clinic inside the Faculty of Veterinary Medicine Bucharest. The assessment of radiological images has revealed changes in acropodial level in 7 horses, ages varying between 8 months and 11 years, of which 4 males and 3 females. Acropodial changes were represented by 3 cases with middle phalanx fracture and the rest with: distal interphalangeal luxation, degenerative processes of the proximal interphalangeal joint, hoof wall exongulation, proliferative processes in middle phalanx.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LIX (1)
Written by Răzvan-Marius VLĂGIOIU, Nicolae CORNILĂ, Gabriela CHIOVEANU, Florica BARBUCEANU

The work aims to investigate the histological changes produced by the parasite Varroa destructor in bee species Apis mellifera, using as a liquid fixative Dubosq-Brasil and Masson modified coloration.

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Published in Scientific Works. Series C. Veterinary Medicine , Vol. LIX (1)
Written by Alexandrina (TURCU) ALECU

In this paper it was conducted a study on four types of antigens prepared from Mycobacterium avium subspecies paratuberculosis cultures in order to build an indirect ELISA method with diagnostic sensitivity as high as possible. The optimization of the method's parameters method was developed together with the interpretation's identifications of the cut-off limit. It was found that the antigen Ag 2, is most appropriate for detecting the level of antibodies in sheep serums, is followed that performant by the antigen Ag 3. In testing the serums of 60 sheeps the antigen Ag 2, obtained by molecular filtration, resulted in a diagnostic accuracy of 78.83% compared with the method of commercial kits.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LIX (1)
Written by Claudia PREDA, Nicolae DOJANA

It was determined the evolution of the levels of calcium and phosphorus from blood plasma in White Plymouth Rock hens (a hen breed with high egg production) and White Cornish hens (a hen breed with a low egg production) during the laying cycle, beginning from 22 weeks age up to 40 weeks of age. Parallel, it has been monitored the plasma evolution of the parathyroid hormone (PTH) and vitamin D levels, and the evolution of the following blood parameters: total protein, albumin, and uric acid. The results relive significant differences, according to the breed, concerning the parallel raising of the plasmatic calcium levels and the laying egg percent. Thus, in Plymouth Rock (PLR) hens, the level of calcium (in mg/dL) raised from 10.5 at the beginning of the laying cycle to 33.3 in the peak of the laying, decreasing then, to 30.9 toward the end of the laying cycle. On the other hand, in Cornish (CRN) hens, at the same moments, the values of the plasmatic calcium were: 8.8, 22.5 and 20.4, respectively. The calcium/phosphorus ratio presented an ascendant evolution in both, PLR and CRN breeds, indicating an increasing of the free calcium content of the blood plasma. Plasma albumins ranged between 17.2 and 22.2 mg / mL in the PLR hens and between 19.8 and 22.8 mg / mL in the CRN hens, with significant differences between groups. Uric acid plasma levels have evolved relatively parallel to the laying percentage, showing an intensified protein catabolism, according to laying percentage, in PLR hens. Analysis of the hormone evolution relieves a peak of the PTH level in PRL hens, around 32 weeks of age (amounted to 353 pg/mL). This peak of PTH is behind the laying peak and it is significantly higher in PLR hens than in CRN hens (185 pg/mL at the age of 36 weeks). Regarding vitamin D, its plasma level presented a relatively constant evolution in both hen breeds, seeming to be not influenced by breed or high metabolic requirements that characterize a lay peak. It can be concluded that the high demands of calcium and phosphorus export during the laying cycle in hens with high egg production are supported by high levels of PTH, the main hormone involved in regulating the homeostasis of these two minerals.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LIX (1)
Written by Manuela STĂNESCU (PASCAL), Monica Elena BURAC, Alexandru Ilie DIACONESCU, Dorin TOGOE, Alexandru VITALARU, Alin Ion BÎRTOIU

Analgesia is the main concern regarding pacient’s postoperative rehabilitation. The main aim of this study was to compare the analgesic effect of Robenacoxib and Tramadol when administrated after surgery. Forty client-owned dogs undergoing genital surgery at the Clinic of Obstetrics and Gynecology (Faculty of Veterinary Medicine of Bucharest) were taken into study. Anaesthetic and supportive care protocols were standardized. Tramadol (2 mg/kg) was administrated postoperatively every 6-8 hours, while Robenacoxib (1 mg/kg) was administrated once a day. Pain scores were estimated according to Glasgow scale of pain for animals. Patients in Tramadol group (n=20) were more quiet (15/20), they ate sooner after surgery (10/20), fewer of them cried and whimpered (10/20). Dogs in Robenacoxib group (n=20) did not lick around the incision line (17/20) and the wound healed faster (15/20). Tramadol alone provides longer-lasting analgesia compared to Robenacoxib, but does not have the same anti inflammatory effect. Robenacoxib has a better effect in wound healing. The combined administration of Tramadol and Robenacoxib should be the subject of a further study.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LIX (1)
Written by Răzvan-Marius VLĂGIOIU, Nicolae CORNILĂ, Gabriela CHIOVEANU, Florica BARBUCEANU

The work aims to investigate the histological changes produced by the parasite Nosema spp. in bee species Apis mellifera, using as a liquid fixative Dubosq-Brasil and Masson modified coloration.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LIX (1)
Written by Violeta ENĂCHESCU, Mariana IONIŢĂ, Ioan Liviu MITREA

Serodiagnostic of Neospora caninum infection in cattle is generally based on using the enzyme-linked immunosorbent assay (ELISA) method for detection of specific antibodies in serum samples, but the use of milk is also possible. The present study was undertaken to assess the potential of an ELISA kit for testing individual and pooled milk samples in dairy farms. Pairs of milk and blood samples (n = 60) were collected from 3 dairy farms (A, B, and C) in southern Romania. Additionally, four pooled milk samples were obtained (one pooled milk sample for each farm and a total sample as a pooled sample from all three farms). Skimmed milk was obtained and tested by using a commercially available ELISA kit (HerdChek N. caninum Antibody Test Kit, IDEXX Lab.). The optimized cut-off value of S/P> 0.704 was determined by receiver operating characteristic (ROC) analysis, using serum results as ‘gold standard’. The sensitivity and specificity of the assay at this cut-off were 70.4% and 100.0%, respectively and the agreement with classic serology, expressed as kappa values, was good (K=0.723). When samples with low positive response on sera were excluded, the correlation obtained was even better (K= 0.921). For pooled milk samples a lower cut-off was necessary in order to identify as positive all dairy farms with a 15% or higher within-herd seroprevalence. The results of this study demonstrate that the prevalence of N. caninum in dairy farms can be estimated by using this indirect ELISA kit on individual and pooled milk samples.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LIX (1)
Written by Galina SIMEONOVA, Dinko DINEV, Emil SLAVOV, Krasimira HALACHEVA

It is well established that major surgery, anaesthesia, and sedation compromise a wide range of immune function that may predispose patients to postoperative infections, septic complications, and tumour spread. The immunosuppressive effects of general anaesthesia are quite different and depend on the used anesthetic agents, dose and combinations. We tested the hypothesis that perioperative lymphocytopenia is due to apoptosis of these cells induced by balanced anesthesia. The relation of lymphocyte apoptosis to the anaesthesiological stress and concentrations of the main pro-and anti-inflammatory cytokines was also investigated. Based on the results we concluded that balanced anaesthesia used in the present study induced lymphocytopenia by activation of apoptosis of these cells which was due to the combined apoptogenic effects of halothane, fentanyl, and pancuronium, but neither to the anaesthesia-related stress-response nor to changes in the main pro-and antiinflammatory cytokines TNF-alpha and IL-10. Total lymphocyte count was diminished on the expense of B-lymphocytes without significant changes in CD 5+ and CD 8+ cells. Clinical implications: balanced anaesthesia disturbs normal humoral immune response by decreasing the count of Blymphocytes for minimum 24 hours after anesthesia.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LIX (1)
Written by Florin Catalin MLAGIU, Alin BIRTOIU

In the past decades, the downsize of the fertility in the milk cows stock farm has become a more and more acute problem. This downsize being caused by a series of factors and reasons such as: genetic improving, inadequate nutrition (deficient or excessive diet), deficient management of reproduction, raise of diseases rate due to immunity abasement, growth technology wrongly applied and the overall well-being of the animal. In the paper the following are presented: causes, etiology, cause-related mechanisms of under and over feeding, vitamins and minerals substances deficient, involved in causes which determine nutritional infertility. Of the highest importance is ensuring the corresponding proportion of each feeding principle. Cows with large milk production are predisposed to genitalia diseases due to metabolic overload. In zootechnical exploitations over 60% of the infertility cases are due to unbalanced diets, so that the protein deficiency from food disturbs the hypothalamic-hypophyseal activity, which leads to the deficit of gonadotropic hormones creation, causing hypoesthesia, apparition, anovular heat. Infertility determined by insufficient energetic substances: the lack of fibrous from food causes the decrease of inferior vermilion fat acids, which are the base for steroid hormone synthesis. Infertility caused by the lack of Vitamin A occurs by the retention of fetal tectorium, uterine under growth, anovular heat apparition. The Vitamin D insufficiency can cause dysgravidism. Vitamin E insufficiency can cause placental degenerative processes. Also, Vitamin C is involved in stress related annihilation problems. Microelements insufficiency such as: Mg,P,Ca,Na,K,Cl,S,Fe,Mn,Cu,Zn, will cause several affections such as: anovular heat set up, luteal cysts, placental retentions, uterine involution, anoestrum, weak or ailing conception products.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LIX (1)
Written by Galina SIMEONOVA

The aim of the experiment was to study changes in blood gases, electrolytes, acid-base and some coagulation parameters occurring during long lasting anaesthesia and strangulation obstruction of the small intestine in horses. Five healthy ponies were used for that purpose. They were submitted to 9 hours isoflurane anaesthesia with CRI of dexmedetomidine and ketamine. Strangulation obstruction of the small intestine was performed for 6 hours for histomorphometric investigations and was restored with enteroanastomosis afterward. Arterial and venous blood samples were taken in three periods and investigated parameters were measured. Instead of periodic IPPV oxygenation progressively decreased judged from lowered PaO2 and saturation with time. Moreover, a respiratory acidosis developed at 3rd hour and went deep at 9th hour. With regard to coagulation system alterations affected only D-dimer. Increase in D-dimer values corresponded with elevation of blood lactate level. Long lasting anaesthesia and abdominal surgery does not impaired significantly oxygenation and coagulation in healthy horses but could contribute to further worsening of already compromised functions in colic horses.

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