Published in Scientific Works. Series C. Veterinary Medicine , Vol. LIX (1)
Written by Alexandrina (TURCU) ALECU
In this paper it was conducted a study on four types of antigens prepared from Mycobacterium avium subspecies paratuberculosis cultures in order to build an indirect ELISA method with diagnostic sensitivity as high as possible. The optimization of the method's parameters method was developed together with the interpretation's identifications of the cut-off limit. It was found that the antigen Ag 2, is most appropriate for detecting the level of antibodies in sheep serums, is followed that performant by the antigen Ag 3. In testing the serums of 60 sheeps the antigen Ag 2, obtained by molecular filtration, resulted in a diagnostic accuracy of 78.83% compared with the method of commercial kits.
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antigens
