Paper presents the progress of the project NOW A STRUDENT! TOMORROW A PROFESSIONAL! – THE IMPROVEMENT OF LABOUR MARKET INSERTION OF STUDENTS ENROLLED IN HIGHER TECHNICAL/ ECONOMIC/ VETERINARY EDUCATION, co-financed by European Social Fund through the Human Resources Development Sectoral Operational Programme 2007-2013, implemented by University Politehnica of Bucharest, University of Agronomical Sciences and Veterinary Medicine Bucharest, University ' Lucian Blaga” Sibiu, University 1 December Alba Iulia, Romanian American University, Craiova University, Ovidius University Constanta. The project aims to facilitate and improve labor market insertion process and working skills development of a number of 10,000 students enrolled in higher technical / economic / veterinary medical education programs by developing and providing integrated guidance, professional counseling and development partnerships with 500 potential employers.. Relevant information about the project can be found at the address: www.proiectimpuls.ro

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Raluca NEGREANU, Radu CONSTANTINESCU, Florin GROSU, Dan CRÎNGANU

Prostate cancer is a malignant disease with high incidence in dogs aged over 10 years, favored by sexual hyperfunction associated with a high protein diet. Chronic inflammatory lesions (prostatitis, cysts, etc.) are precancerous lesions and undiagnosed and treated early can degenerate into malignant lesions. Diagnosis is based on laboratory tests: ultrasound, urine cytology, contrast radiography, puncture aspiration through ecography or trans-perineal, blood works and specific detection of tumor markers. By using several diagnostic methods we are trying to establish the baseline of malignization with the beneficial consequences of establishing early, curable therapy protocols with consequent prolongation of survival and increased comfort. Early screening for diagnosis and treatment of various diseases, like chronic inflammatory lesions (prostatitis, prostatic cysts, etc.) may prevent malignancy or even block or allow the detection of the process of cancerization in early stages with decreasing tumor metastasis and reduce the chances of suffering patients.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Niculae TUDOR

Here we present the radiographic findings of limb and spine in a 15-month-old female Bichon with pseudoachondroplasia (PSACH). PSACH is a rare form of osteochondrodysplasia, the main clinical characteristics are disproportionate short stature, abnormalities of the limbs and spine, abnormal walk, joint laxity and early osteoarthrosis. The animal was evaluated by physical and radiographic exams. Radiographic images of the skull, spine and limbs were obtained, and showed normal appearance of cranial structure and multiple changes in the limbs and spine, including generalized dysplasias of epiphyses and metaphyses of the long and short bones. Epiphyses were incomplete developed and had irregular appearance, separated by a radiolucent line, and metaphyses of the long bone were prominent flared appearance. Carpal and tarsal bones were also affected, showing shape changes.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Florin STAN

Metastasis, the spread of tumor cells from the primary site to lymph nodes and the distant organs is the most aggressive and specific feature of malignant cancer. The mechanisms by which malignant cells leave the primary tumor, invade lymphatics and metastasize are complex and interconnected being directly related to biological behavior of tumor. However, the lymphatic vasculature is often neglected. The aim of this study is to establish if there is a correlation between the peritumoral and intratumoral lymphatic vascular density and the presence of metastatic infiltration in sentinel lymph nodes of mammary gland tumor. Injecting the coloring solution in mammary gland tumor of nine female dogs it was noted the pattern of lymphatic vessels at the injection site, their density, size distribution area, their trajectory to the first lymph node. Also the status of the tumor draining lymph node was histological assessed. The architecture and density of intratumoral and peritumoral lymphatic vessels was determined by their function in absorbing interstitial fluid together with the tumoral cells, due to their permeability. The coloring solution show the sinuous pattern of peritumoral lymphatic vessels, with a rich chaotic vascular network compared with reticular or plexiform pattern of lymphatic vasculature of healthy mammary gland. The size of peritumoral lymphatic colored area was dependant on the histological type of the tumor and on its size. Also, a malignant tumor size >1cm was associated with the presence of the metastatic infiltration in the first tumor draining lymph node. The density of intratumoral lymphatic vessels was low compared with the peritumoral lymphatics. In conclusion, qualitative morphological assessment of lymphatic vasculature of malignant mammary gland tumors of female dog revealed an increased density of lymphatic vessels in the peritumoral region and a lesser degree intratumoraly. The size of peritumoral lymphatic area was directly related with the presence of metastases in sentinel lymph nodes. Although great progression has been made in revealing the lymphangiogenic markers, additional studies are required to understand the paradoxical significance of intratumoral and peritumoral lymphatics density and lymph nodes metastases for prognosis and development of metastases in vital organs.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Andra ENACHE, Pip BOYDELL, Iuliana IONAŞCU, Alexandru ŞONEA

This report describes conjunctival ballooning as a result of subconjunctival accumulation of the injected fluid following retrobulbar block for intraocular surgery in the dog. A prospective study was conducted on seventeen cataract procedures in dogs of different breeds, weighing between 6.1 kg and 33 kg, aged between 12 weeks and 8 year old where retrobulbar nerve block was performed prior to surgery. Local anaesthetic (Lignocaine hydrochloride injection BP 2%) was diluted with different volumes of saline (2-5 mls) and the solution was slowly infiltrated into the orbit via a ventrolateral conjunctival approach until the globe was displaced anteriorly into a central gaze position. The purpose was to obtain a good eyeball positioning prior to phacoemulsification procedure. In 3 cases a subconjunctival ballooning was noticed with a doughnut appearance of the bulbar conjunctiva that precluded surgical access to the dorsal cornea. These cases required the use of fine scissors to make a radial cut in the elevated conjunctiva, until the swelling was reduced sufficient so as not to interfere with the surgical procedure. This communication records conjunctival ballooning as a complication of retrobulbar nerve block in the dog.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Iulia PARASCHIV, Laurentiu TUDOR, Manuella MILITARU

Introduction: Budgerigars are submitted to several pathologies, including neoplasia. Cytopathologic examination is quick, easy and with minimal risk for the pacient and psittacines can be candidates for this diagnostic technique. The aim of this study is to evaluate the relevance of the cytopathologic diagnosis on fibrosarcomas in budgerigars. Materials and methods: 3 cases of Melopsittacus undulatus were submitted to multiple cytopathological examinations at the Department of Pathological Anatomy of Faculty of Veterinary Medicine, Bucharest during November 2011 - July 2012. The cases were also submitted to histopathologic examination and confirmed the diagnosis of fibrosarcoma. 10 high-power fields were examined in each of the several smears obtained by fine needle aspiration technique. The categories of cells numbered are: neoplastic cells, erythrocytes, inflammatory and a category of smashed cells and nude nuclei and numbers were afterwards turned into percentages. Results and conclusion: Neoplastic cells were identified with a medium percentage of 17.57%, while inflammatory cells were 2.98%, erythrocytes, 57.65% and smashed cells, 21.80%. The diagnosis of malignancy was made with extreme caution due to the low number of mesenchymal cells, but it was also taken into consideration the high percentage of pollutant erythrocytes. Based on the malignancy cellular features, the neoplastic cells presented anisocytosis, pleomorphism, increased nucleus:citoplasm ratio and coarse chromatin pattern. Additional, case 2 presented frequent macrocytosis, macrokaryosis (40.38%), multinucleation and visible, abnormal nucleoli (1-4) were encountered. Rare, abnormal mitosis were identified. As a conclusion, cytopathologic diagnosis of fibrosarcomas in budgerigars (Melopsittacus undulatus) can be challenging based on the risk of pollutant erythrocytes, but, ignored, the smears offer multiple cellular malignancy features that plead for fibrosarcoma

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Andrei TANASE, Adrian COMARZAN, Catalin MICSA, Marius Mihai Muste

Hip fractures in cats occupy a significant share in traumatology and bone diverse area . Comparative advantages and disadvantages are described using cerclage fracture remedy to the method of fixation with plate and screws. The results presented are obtained from the method on four cases of fracture fixation with plate and remedied by screws, 11 cases of pelvic fracture fixation remedied by cerclage and 2 cases of bilateral fracture of the iliac blade which have been corrected compared to the two methods . The study shows that the method is more laborious but cerclage better protect musculature area and gives a very good resistance .

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Catalin MICSA, Dorin TOGOE, Andrei TANASE, Alexandru SONEA

Laparoscopic surgery gained a lot of field in human and veterinary medicine, replacing succesfully many invasive surgical techniques. Laparoscopic surgery presents several medical advantages, but equally has a high risk of bleeding accidents (rupture of the vascular pedicle, incorrect ligation or application of vascular clamps). The aim of the study was to illustrate the way to remedy this intraoperatory accidents. Research was carried out on 5 sows that were ovariectomized laparoscopically as an experimental model for human medicine. For addressing these accidents, it can be intervened with lavage (irrigation-aspiration), individualization of the vascular pedicle, pressure and clamping with metal clips or external ligatures in order to completely stop the bleeding. Our study showed the real capacity of this methods to control small and medium intensity haemorrhages, without having a negative impact on surgery outcome and recovery period.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1
Written by Marian GHIȚĂ, George Florea TOBĂ, Gabriel COTOR, Leonard George TOBĂ

Our researches are aimed to determine the amplitude of the recorded electrocardiograph waves by means of three leads systems (two systems found in the scientific literature, and a leads system imagined by us), of a dairy cow. To this end, there were recorded electrocardiograms on group of 20 dairy cows and then the electrocardiograms were interpreted by calculating the amplitudes of the waves P and T and the total amplitude (summing the positive and negative for each branch in part) of the ventricular complex QRS, from the all three leads systems used. After interpreting the obtained results, we can conclude that neither of the leads system used in our study provides a complete electrocardiographic investigation. Thus, derivations Dubois may be recommended for recording ECG in D II (bipolar derivation II), D III (bipolar derivation III) and aVF (unipolar derivation, with electrode placed on the left hindlimb), while recording electrocardiogram in D I (bipolar derivation I), we recommend using limbs leads and leads system designed by us. Regarding recording of the electrocardiogram in aVR (unipolar derivation, with electrode placed on the right forelimb) and aVL (unipolar derivation, with electrode placed on the left forelimb) we appreciate that all three systems examined in our research can be successfully used.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Ovidiu SAVU, Alexandra OPRESCU, Manuella MILITARU

This study is necesary for preveting feedind the hens with feed that is contaminated with bacteria from the Salmonella genus to prevent the transmission of diseases caused by this kind of bacteria and to monitor the microbiological quality of the rations in order to improve food safety. Due to Salmonella implications in importat economical losses and their implications in human health by triggering food poisoning from eating contaminated products makes this one of the most pressing issues in veterinary medicine. There are infectious diseases caused by pathogens of the genus Salmonella. They are universal spread and specific zoonoses, some strains are ubiquitous, and others with regional character. In terms of food poisoning caused by Salmonella we can say that, in terms of frequency and sanitary implications, they occupy an important place in most countries of the European Community. The possibility of human infection is constantly growing in the context of the circulation of contaminated food. The disease can be triggered by eating powdered products with an increased shelf life. Microorganisms are present in feed, soil and surface water. In birds that carry it, the bacteria are located in the digestive tract, gall bladder, ovary and they can eliminate it in the environment all lifelong. Specific serotypes are represented by S. enteridis and S. typhimurium. Venue of the study was the unit S.C. Avicola Găești S.R.L. Measurements were taken on samples of feed and fodder, harvested amount is 1kg / sample, the sample is then divided into 5 units of equal mass. The method of investigation used was the Horizontal method for the detection of Salmonella spp. - SR EN ISO 6579:2003 / AC 2009, a method that is RENAR approved. At the end, measurements found no pathogens in samples of feed and fodder (Absent / 25 g). Using this method, it proved one of the most important aspects, that of the desire to obtain safe and quality products coming from the poultry sector.

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