Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Raluca NEGREANU, Dan CRINGANU, Alexandru VITALARU, Razvan NEGREANU

In order to have an early diagnosis and to apply a corect and complex treatement we have followed the evolution of this disease with 3 actions: using modern technologie, strict treatement protocol and optimized ways of administration. The protocol for the antineoplastic therapy must be decided after the corect assessment of the TNM, after the complete blood work and after the x-ray, Eco and MRI results. When choosing the chemotherapy there must also be taken in consideration the type of drug used, the path of action and the path of elimination specific to it in order to prevent the cumulative effect

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Florin Ştefan HORA, Narcisa MEDERLE, Corina BADEA, Elena Mihaela TILIBAŞA, Marius Stelian ILIE,Gheorghe DĂRĂBUȘ

During the period November 2013 - February 2014, 24 adult hunted hare (Lepus europaeus) from four hunting sites of Arad County were subjected necropsy. Of them, 13 were males and 11 females. The gastrointestinal mass from each was examined to determine the digestive parasites, macro- and microscopic examination were performed. Each segment of the digestive tube was sectioned, the mucosa and the gastrointestinal content were carefully examined and for the gastrointestinal mass successive washes method was used. The gastrointestinal content and also each segment of digestive tube (previously washed) were microscopically examined by stereomicroscope. The faeces found in the large intestine were examined by flotation method (Willis). Of the 24 samples examined, 21 were positive for gastrointestinal parasites, (prevalence of 87.50%). The most prevalent parasitism was with Eimeria spp., found in 17 samples (80.95 %), other parasites identified were: Cysticercus pisiformis found in two samples (9.52 %), Trichocephalus leporis in 13 samples (61, 90 %) and Trichostrongylus spp. in seven samples (33.33 %). The parasitism with gastrointestinal helminths and larval cestodes in hare represent risk factors for rabbits and domestic carnivores

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Adina BĂDICU, Iuliana IONAȘCU, Alin BÎRȚOIU

Endoscopic cyclophotocoagulation (ECP) is a relatively new method of cyclodestruction in the treatment of glaucoma, by lowering IOP through aqueous suppression. Although the coagulation of the ciliary processes using laser is well documented, this new endoscopic approach has numerous benefits comparing to the transscleral cyclophotocoagulation. The paper aims to present a review of the indications, techniques and efficacy of diode ECP. ECP uses a diode laser equipped with an endoscope which permits direct localization and photocoagulation of the ciliary processes. The procedure can be combined with phacoemulsification in patients with coexisting cataract. Other indications of diode endolaser are iridal mass, ciliary body neoplasia, uveal cysts and retinopexy in retinal detachment cases. The available clinical evidence reviewed suggest that ECP is a safe and effective procedure in veterinary ophthalmology.

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Sacalin Island is an area of major importance because it is situated in the path of one of the most important bird’s migration routes in the world, covering three continents: Asia, Europe and Africa, the route with a great abundance and biodiversity of avifauna including sedentary species, passage species, and summer and winter guest species. In order to demonstrate the necessity of preserving this area and to establish the biodiversity of ornithological fauna, this article presents the results obtained from the data collection in a period of 6 years and analyzed in terms of the status, abundance and biodiversity of birds, highlighting the need to protect this habitats. Our result showed that the birds collected form Sacalin Island belonging to a wide variety of birds including 17 orders, 46 families and 201 species; 53% of the total of 382 bird species identified in our country. The frequency of bird populations in relation with biogeographic regions during winter and vernal season showed that the dominant species are the species typical for Palearctic regions. By analysis of trophic level, our results showed that there is a definite dominance of insectivore species (72%), followed by carnivore (9%), omnivore (5%) insectivore – larvivore (4%), and larvivore (2%) species. Summarizing all the results it is clearly that Sacalin Island should maintained the strictly protected area status; here are present 85% of strictly protected bird’s species.

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Iudith IPATE, Alexandru BOGDAN, Simona IVANA, Nicolae IPATE, Lucian ALECU, Janos SEREGI, Janos TOSSENBERGER

An anthropozoonotic agent whose circulation is mainly determined affects the food complex managerial concerns. In the last 10 years Romania reported over 788,000 foods borne illness associated. The cost of medical therapies has increased considerably as determined by laboratory investigations for accurate etiological required. New rules were adopted security strategy taking into account the movement of aggressive agents and their potential contaminating the food. The pragmatic complex factors of ecosanogenesis contributing to the interdisciplinary vision in relation: human - product – nature. Redesigning techniques and technology, and management will lead to gradual replacement of the current economic guidelines with other levers that converge for example by minimal pollution, but also to the prices of raw materials and stimulating savings, reduction of energy consumption etc. Meeting the nutritional and sanitary quality requirements must adapt to new regulations to ensure consumer prerequisite – compliance. Enhancing food movement - national, regional, global - through trade creates new opportunities contaminant level amplification and diversification of pathogens.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by EFFECT OF VARIOUS LEVELS OF DIETARY CALCIUM ON BLOOD CALCIUM CONCENTRATION AND HORMONAL STATUS IN WHITE CORNISH AND WHITE LEGHORN HENS

Two hen breeds, White Cornish (COR) and White Leghorn (LEG) 34-week-aged were used in this experiment. The basic diet of these hens contained 3.8% Ca. This diet was supplemented for an experimental period of 8 days with 0.0, 1.0 and 2.0 and, respectively, 3.0% calcium. During the experimental period the plasma blood levels of Ca, phosphorus, parathormone, calcitonin, vitamin D and estradiol were monitored. The results relive a significant increase of the blood plasma levels of Ca and phosphorus in both COR and LEG hens beginning form the 2% calcium dietary supplementation. Phosphorus levels increased too, but the Ca/P ratio indicated an imbalance for the calcium. In the same time it was found a significant decrease of the plasma level of parathormone in both 2% and 3% Ca supplemented CRG and LEG hens. Blood plasma level of calcitonin doesn’t follow a clear increasing in any experimental group, neither for CRH nor for LEG hens: statistical analyses between day one and 8th day of the experimental periods didn’t relive any statistical significant difference in any experimental group for this hormone. Plasma vitamin D concentrations followed a statistical significant (P<0.05) increase for both 2% and 3% calcium supplemented diets in both CRN and LEG hens. Estrogen levels were found significantly elevated in LEG hens vs. CRH hens, but the differences were not in relation to the blood calcium concentrations. In conclusion, blood calcium levels in hens influences the parathormone and vitamin D secretions but its excact influence on the calcitonin and estrogens is not clear. Further experiments are recommended to find the exact mechanisms of blood calcium regulation in birds.

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Cytostatic chemotherapy induces changes in the homeostasis of the hepatic DNA and of serum proteins. Among the various drugs used in the chemotherapy the alkilating agents, antimetabolites, steroid hormones and antibiotics are better known. In the last decades there were discovered other new compounds such as platinum derivates which representia distinct class of compounds, having specific antitumoral action. Among platinum derivatives an essential effect has cis-platinum. Research on the cytostatic activity of cis-platinum implies the knowledge of the pharmacokinetic and biochemical effects. Experiments "in vivo" performed on Wistar strain rats pursued the action of the intraperitoneally injected cis-platinum on the hepatic deoxyribonucleic acid (DNA) biosynthesis and on serum proteins. The statistically processed analytical data revealed non-significant decreases of the hepatic DNA and of serum proteins concentrations. Electrophoretical fractions showed hypoalbuminemia and hyperglobulinemia. In case of globulin subfractions the decrease of alpha 1 - and alhpa 2 - and increase of beta - and yota - globulins was observed.

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Laura PARLAPAN PÎNDARU, Mihai CENARIU, Emoke PALL, Ioan Ștefan GROZA

Currently, there is a growing interest on cryopreservation of boar semen even if the sperm membrane of swine shows high sensibility to this process. Previous studies have shown that the cryoconservation has an oxidative degradation effect, associated with the production of reactive oxygen species (ROS). ROS cause lipid peroxidation in sperm membranes and a variety of physical and chemical changes of sperm cells that predispose to DNA damage and apoptosis. The only alternative to counteract the effects of ROS is the addition of various antioxidants in lipid storage extenders. Starting from this premise, this work investigates Coenzyme Q10 effects on the viability of preserved boar semen during liquid storage. Semen was randomly divided into 5 groups and treated with different concentration of Coenzyme Q10 and storage at 17ºC for 5 days. The viability of semen was evaluated every day, using flow cytometer FACSCanto II (BD Biociencias) systems. The samples for FACS were labeled with Hoechst 33342, floresceinisothiocyanate conjugated with peanut agglutinin and propidium iodide. These experiments indicate that supplementation of Coenzyme Q10 to the semen extender can increase the sperm characteristics and prolong the survival of liquid storage semen, which may have potential benefits in reproductive biotechnology field.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Costin BUDICA, Rosalie DOJANA, Laurent OGNEAN, Iuliana CODREANU, Nicolae DOJANA

In the frame of this paper it was researched the relationship between season and some reproductive parameters (oestrus length, pregnancy length and total born piglets) in primiparous and multiparous sows in terms of the photoperiodicity climate in Romania. The research was conducted on a crossbred Yorkshire sows (♀) × Landrace (♂) sow population and consisted of monitoring the oestrus duration, the gestation period and the number of total born piglets related to astronomical seasons: fall, winter, spring and summer. They were found seasonal influences characterized by a longer gestation length in autumn and spring seasons vs. summer and winter, with a difference of about one day between the seasons, and an annual average difference of about one day between primiparous and multiparous sows. Gestation length was inversely correlated with total born piglets. The oestrus length had an annual average of about 2.16 days in primiparous and 2.98 days in multiparous sows, with peak values in seasons of growing photoperiodicity and minimum values in seasons of decreasing photoperiodicity.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Costin BUDICA , Rosalie DOJANA, Laurent OGNEAN, Gabriel COTOR, Nicolae DOJANA

The research was conducted on a breed of adult sow in different physiological states, originated from a northern EU area, during the period of adaptation in a temperate (Romanian) area. The animals were in different physiological status: gilts and sows, estrous, pregnancy or lactation. They were determined the blood plasma levels of main hormones involved in the reproductive function [17-estradiol, progesterone, follicle stimulating hormone (FSH), luteinizing hormone (LH) and melatonin] in the days of the solstices and equinoxes. Plasma levels of 17- estradiol for gilts and sows during the first day of the estrous showed minimal values in March, 20th, increased in June 21st, reaching a maximal values in September, 22nd, and then decreased again. The LH mean values (assayed in the first day of estrous period) were highest during the maximum photophase (June, 21st) and lowest in September, 22nd in gilts, while in sows, the higher levels of plasma LH were found during the day of spring equinox and summer solstice and the lowest levels in September, 22nd (as in gilts). Plasma progesterone of the 25-day-pregnant gilts and sows presented the highest values during the period of maximum scotophase (December, 21st), significantly higher by comparing to the other three analyzed photoperiods. Plasma progesterone of pregnant gilts was lower vs. pregnant sows for every analyzed photoperiod. The maximum amounts of melatonin blood values were found in December 21st, the lowest in June 21st and intermediate values in the two solstices. For all the assayed photoperiods, melatonin contents in midnight samplings were nearer to those taken at midday. Almost every time, the melatonin values in lactant sows were lower vs. in pregnant sows.

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