Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIII, Issue 2
Written by Andrei TOPOLICEANU, Mimi DOBREA, Gabriel GAJAILA, Mara GEORGESCU, Gabriel MURARIU

Four starter cultures used in dairy industry were investigated in this study for their acidifying activity. Two of them are mesophilic cultures R-703 and R-708 and the other two are thermophilic cultures FRC-60 and FRC-75. These cultures were inoculated in sterile cow milk and incubated at 32°C (mesophilic cultures) and at 45°C (thermophilic cultures). The pH value was determined every 2 hours. The acidification profile of all cultures was similar, but the termophilic cultures produced a faster acidification (after 4 hours), than the mesophilic cultures (after 6 to 8 hours). After 10 hours of incubation the pH value of the medium for all studied cultures was stabilized around 4.5.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Ioana DUMITRU, Cristian MARTONOS, Bianca MATOSZ, Flaviu Tuns, Florin SILAGHI, Aurel DAMIAN

Although the predominant species found in poultry farming are galliforms and anseriforms there are few comparative studies referring to the skeletal morphology of these species. This study compares thoraco-abdominal component of these species in light of their different adaptation to the environment. The research was carried out in the Compared Anatomy laboratory of the Faculty of Veterinary Medicine of Cluj-Napoca. A total number of 6 skeletons, 3 chickens and 3 gooses were examined following comparative aspects of the anatomical structures relevant for this area. After examining the bones from the thoraco-abdominal region, differences as well as similarities between the two species of birds were observed. The thoracic cavity in chickens presented 7 pairs of ribs, of which 2 were asternal, while the same segment in geese contained a number of 9 ribs of which only 1 asternal. The sternum, presented 3 pneumatic holes in chickens and only one in geese. Also, differences were noted at the level of the sternal body, which was reduced in the chicken compared to the goose. On the lateral sides of the sternal body, the goose registers 7 or 8 costal indentations depending on the individual, compared to only 3 found in the chicken. The coxo-dorso-lumbosacral complex presented small differences between the two species. The iliac fossa in chickens is well defined, the pubis is oriented dorsally, slightly curved medially and the coccygeal vertebrae are 7 to 8 depending on the individual. In geese, the external iliac fossa is absent, the pubis is oriented ventrally, curved medially and the coccygeal vertebrae are 6, the pygostyle being fused to the last vertebrae.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIV, Issue 1
Written by Georgi MARINOV, Nadya ZLATEVA-PANAYOTOVA, Krassimira GENOVA

The use of anesthetics is an integral part of any surgery. Despite their widespread use, their mechanisms and interactions with the nervous-endocrine and immune systems are insufficiently studied.The study was conducted with healthy, adult cats subjected to anesthesia and surgery (ovariohysterectomy) in order to trace the effect of anesthesia and surgery on the secretion of proinflammatory cytokines IL1, IL6 and TNFα. The ovariohysterectomy was performed when a deep plan of anesthesia occurred. Blood samples were obtained at 0 min (prior to anesthetic administration), 30, 60, 120 min and 24 h.The chosen anesthetic schemes modulate the immune response and the response depends on the type of anesthetics used.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIII, Issue 2
Written by Alina Maria IONESCU, Maria TOADER, Mirela Elena DUȘA, Emil GEORGESCU

This paper presents the results of the research on the yield quality of some grain legumes (lentil, faba bean, chickpea, fenugreek, mung bean and adzuki bean) promoted by organic agriculture. In order to determine the quality of grain legumes, there were analysed several physical indicators, such as the thousand grain weigh (TGW), the hectolitre mass (MH), the moisture content (%) and chemical indicators, such as the protein content (% d.m.), the carbohydrates content (%), the fat content (% d.m.), the ash content (%). The seeds energetic value (Kcal.%) was calculated as well. In order to determine the TGW, 8 samples of 100 seeds were weighed, while to determine the MH, the Hectolitre Measuring System - Chondrometer, with a capacity of 0.5 l, was used. The following methods were used for the determination of the biochemical compounds of seeds: for carbohydrates - the Bertrand method; for proteins - the Kjeldahl method; for fats - the Soxhlet method and for ash - the Spectrophotometer method. On average, the chemical composition of these crops was the following: for lentil - 22.50% proteins, 2.81% fats, 63.56% carbohydrates, 3.95% ash, and an energetic value of 358.47 kcal.; for faba bean - 21.60% proteins, 4.45% fats, 63.90% carbohydrates, 5.90% ash, and an energetic value of 366.77 kcal; for chickpea - 21.19% proteins, 4.30% fats, 56.17% carbohydrates, 3,31% ash, and an energetic value of 356.16 kcal; for feenugreek - 21.24% proteins, 4.66% fats, 63.81% carbohydrates, 5.71% ash, and an energetic value of 360.58 kcal.; for mung bean - 23.23% proteins, 2.08% fats, 68.09% carbohydrates, 3.88% ash, and an energetic value of 362.89 kcal.; for adzuki bean- 21.9% proteins, 2.6% fats, 69.3%carbohydrates, 4.1% ash, and an energetic value of 361.14 kcal. The nutritional value results for these grain legumes highlighted the very special role that they should play in the development of biodiversity as well as in the diversification of human and animal feeding.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIII, Issue 2
Written by Georgiana DUŢU, Adina ARGĂSEALĂ, Lia ION, Emilia CIOBOTARU, Iuliana IONAȘCU

The study aimed to present the importance of the proper treatment in Keratoconjunctivitis Sicca in dogs depending on the evolution stage, approaching all the phases. It highlights the necessity of an urgent and accurate treatment in order to avoid blindness. The study is based on the clinical activity of The Department of Surgery from The Faculty Of Veterinary Medicine of Bucharest, during 18 months. During the analyzed period were evaluated 472 dogs with keratoconjunctivitis Sicca, divided in three groups according to the evolutive stage. The treatment used the following products: for the first phase, artificial tear products based on hialuronic acid and carbomers such as HyCare®, Vidisic® and Xanternet®; for the second phase were used artificial tear products and cyclosporine 2% and for the third phase the treatment consisted in eye drops with prednisolone and ointment with tacrolimus. The healing process was complete in 4 to 24 weeks depending on the perseverance of the owner in administrating the treatment and the severity of the disease.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIII, Issue 2
Written by Raluca M. MUNTEANU, L.O. SCOICARU, Manuella MILITARU, Elvira GAGNIUC, Catalin MICSA, Dorin TOGOE, Alexandru B. VITALARU, M.I. RUSU, M. TAUTAN, B. CHIRICUTA, M. V. UDREA, Alexandru SONEA, C-E-A. GRIGORESCU, Alin I. BIRTOIU

This paper reports on two clinical cases where Raman spectroscopy has been employed for diagnostic in dog mammary tumour surgery. The comparison between histopathology and SERS results proves that the spectral markers can be used to distinguish between malignant or normal tissue. By comparing results from histopathology and Raman spectroscopy we set up a fast and affordable diagnostic tool using the marker-like Raman bands of carotenoids and respectively interfacial water. Application of the described technique is useful in comparative studies of canine and human mammary cancer.

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This paper presents a liquid-chromatography negative ion electrospray tandem mass-spectrometry (LC-MS/MS) method to determine resorcylic acid lactones (RALs) in urine, muscle, fish, liver and kidney samples. The method comprises an extraction step followed by the clean-up of the samples in two steps using SPE cartridges: C18 Chromabond and Strata Amino from Phenomenex. The RALs were separated on a Pursuit C18 column. in isocratic mode with acetonitril/ 0.1% formic acid aqueous solution. The time for analysis was 12 minutes. A triple quadrupole mass spectrometric from Varian equipped with a ESI source in the multiple reaction monitoring was used for detection. The method was developed according to EU legislation and the parameters assessed were: selectivity, specificity, linearity, recovery, repeatability, within-laboratory reproducibility, decision limit (CC), and detection capability (CC), ruggedness, control chart, uncertainty of measurement. The validation was performed at 1μg/kg for each compound. CC and CC values were 0.16-0.79 micro g/kg and the range of mean recoveries were 72.2-119.7% depending of the compound. The relative standard deviation (RSD) of the measurements was below 20%.The method is used to analyze samples originating from the Romanian Residue Control Monitoring Program or private samples. The main purpose of this method is the detection, quantification and confirmation of 6 RAL’s in urine and tissue.

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIII, Issue 2
Written by Andrei Constantin STOIAN, Teodoru SOARE, Iulia Alexandra PARASCHIV, Laura Mihaela DUMITRU, Emilia CIOBOTARU, Gabriel PREDOI

Congenital pericardial diseases are rare in dogs and cats and most part of them were reported as incidental findings on radiography or necropsy examinations. In this case report, a definitive diagnosis of congenital peritoneo-pericardial diaphragmatic hernia (PPDH) in a three year old male mixed breed dog (Canis lupus familiaris) is described. The dog was referred in relatively shock status with a history of anorexia, dyspnea, cough, abdominal distension, progressive weight loss, but died after conservative treatment was applied. At necropsy examination, a cranial displacement of abdominal viscera (portion of right hepatic lobe) into the pericardial sac was observed, while the pleural space was intact. Supplementary, another dog from the same nest/littermates (3-year-old female), which died in identical conditions was submitted for postmortem investigations and showed no congenital abnormalities.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Flaviu TUNS, Alina IURCUT, Ioana CHIRILEAN, Alexandra BLENDEA, Aurel DAMIAN

Previous studies performed in human and swine regarding the segment of ascending aorta and aortic arch identified several differences that referred to the presence and number of aortic collaterals in this region. Other studies mention the intraspecific differences in humans that refer to the way in which the aortic branches and even to their number. Due to the numerous similitudes of the cardiovascular system between humans and swine, we choose this specie to study the intraspecific variations regarding the aortic branches. The objective of this paper was to realize a comprehensive study of the swine aorta, in its initial part, namely the aortic arch, to highlight its systematization and to describe its branches and their count. To highlight the potential particularities found in the initial part of aorta we initially dissected the arteries and then injected them with latex mixed with red pigment to obtain a better view of the blood vessels. The process of latex injection was made by two ways: through the left ventricle and through the abdominal aorta. Following injection, the biological material was fixed in 2% formalin for 48 hours. Through dissection we observed that aorta presents several specie peculiarities in swine. We did not find any intraspecific differences regarding the primitive aorta or aortic arch. The particularities that were found are characteristic to the specie and refer to the presence of an aortic bulb with a calibre similar to that of the aortic arch; the presence of sigmoid valves that were well developed; the absence of the ascending aorta in swine. We also found a left subclavian artery more developed then the one from the right side.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by PETREC OANA, BUCUR IULIA, FLUERASU L., STANCU A.

Avian reovirosis are contagious infection diseases found in broiler chickens and turkeys, raised in intensive sistem. The brolier carcasses were biweekly necropsied , which were collected from two herds in which the disease developed. The presence of malabsorbtion syndrome was confirmed by RT-PCR and ELISA test. At the necropsied chickens were found following macroscopic lesions: deformed feather wings, underdeveloped bones, deformations coasts, necrosis of the femoral head and proventriculitis. Histological lesions present in the proventriculus, small intestine and liver, disrupting the digestion process of feedingstuffs, that explaining the clinical evolution and failure spore by weight in broiler chickens, and histological lesions present in stock Fabricius explains the immunosuppression phenomenon existing in these chickens.

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