COMBINING IN SILICO MODELING AND BLOOD GLUCOSE DATA TO INCREASE THE PERFORMANCE OF INSULIN THERAPY IN DIABETIC CATS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Madalina ROSCA, Adrian BURLACU, Gheorghe SOLCAN

The current study designs a potential mathematical model for insulin dependent diabetic cats, by examining the dynamics of glucose-insulin, based on an adaptive minimal model, commonly used for human investigations. Matlab software was used to develop the in silico environment. The glucose minimal model considered blood glucose concentration, blood insulin concentration, steady state blood insulin concentration and the steady state blood glucose concentration. An adaptation for the insulin minimal model was performed in order to describe exogenous insulin infusion, following the decay rate of blood insulin and the volume of insulin distribution pool. Model functionality was increased by describing glucose level alteration after a meal. In silico modelling demonstrated that it could be used to approximate insulin requirement in insulin dependent diabetic felines, based on the insulin dynamics after being administrated subcutaneously. The mathematical model provided reliable guiding information concerning insulin dosing in diabetic cats. Application of an adapted minimal model is a useful tool to examine glucose-insulin dynamics in cats, as it provides quantitative measure of insulin sensitivity. The predictions generated by the present model can be taken into account for insulin and meal adjustment during treatment and consequently a reduction of hypoglycemia incidence.

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Madalina ROSCA, Adrian BURLACU, Gheorghe SOLCAN 2013, COMBINING IN SILICO MODELING AND BLOOD GLUCOSE DATA TO INCREASE THE PERFORMANCE OF INSULIN THERAPY IN DIABETIC CATS. Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4, PRINT ISSN 2065-1310, 80-83.

COMPARATIVE ANALYSIS OF CELL POPULATION PRESENT IN THE MILK AND THE COLOSTRUM OF ALPINE AND CARPATHIAN GOATS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1
Written by Alina NĂSĂLEAN, Emöke PALL, Sergiu MUNTEAN, Laurenţiu OGNEAN

The analysis of the cell population present in the milk and colostrum of goats represents basic morphological characteristics of milk, through which the health of mammary gland and of the milk intended for the consumers can be determined. The purpose and the goals of these determinations consisted in comparative, quantitative and qualitative evaluation of the cellular content from raw milk and colostrum of goat from two lots of goats, clinically healthy, the breeds being French Alpine (n = 10) and Romanian Carpathian (n = 10). Morpho-physiological investigations were conducted during March-July 2015, on samples of raw milk and colostrum, using Squash technique, panoptic colored (Dia-Quik-Panoptic), and milk cytogram method. Microscopic examination revealed that the milk cytogram of both breeds’ colostrum shows a higher frequency of epithelial cells, lactocytes in various forms of activity, lymphocytes, macrophages. The highest frequency was for neutrophils. The milk cytogram in raw mixture milk indicates that the cell population is very similar for both races, with same increase or decrease in those studied months. The neutrophils reached an average of 45.7% for the Alpines, respectively 46.17% for the Carpathians. There were no significant differences of interest regarding cell population of raw milk and colostrum of both races. In conclusion, the results support the need for correlation between quantitative and qualitative microscopic cytological tests on smears because there are no standards in automated system for goat milk in terms of health assessment of the mammary gland and of safety of the milk meant for consumers.

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NĂSĂLEAN A., PALL E., MUNTEAN S., OGNEAN L. 2016, COMPARATIVE ANALYSIS OF CELL POPULATION PRESENT IN THE MILK AND THE COLOSTRUM OF ALPINE AND CARPATHIAN GOATS. Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1, ISSN 2065-1295, 22-26.

COMPARATIVE ANATOMICAL STUDY OF SWIMBLADDER IN DIFFERENT SPECIES OF FISH

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Kateryna GROM

Fish are the most numerous group of vertebrates in the world and their anatomy is still not completely studied. This article is aimed to present the morphological study of swimbladder in different species of ray-finned fish which have industrial value - namely Russian sturgeon, American paddlefish (order Acipenseriformes), rainbow trout (order Salmoniformes), northern pike (order Esociformes), zander (order Perciformes) - and to compare it’s structure and shape with the swimbladder of common carp (order Cypriniformes). Also the analysis of functions of swimbladder is given. The research was carried out in the Department of Animal Anatomy of National University of Life and Environmental Sciences of Ukraine. The study was performed by anatomical dissection on cadavers of 3 fish of each species with further macroscopic examination of swimbladderes. The research showed that in all investigated species this organ has significant differences and is composed of one chamber. The swimbladder of common carp is composed of two chambers (anterior and posterior). Almost all investigated species of fish have connection between the swimbladder and the gut (pneumatic duct), so they are believed to be physostomes. The study highlighted characteristic features of swimbladder in different species of fish that has practical value for better understanding of fish anatomy and possible swimbladder disorders.

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Grom K. 2015, COMPARATIVE ANATOMICAL STUDY OF SWIMBLADDER IN DIFFERENT SPECIES OF FISH. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 156-160.

COMPARATIVE ELECTROPHORESIS OF SERUM PROTEINS IN RATS TREATED WITH TWO ORALLY DELIVERED LECTINS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by COMPARATIVE ELECTROPHORESIS OF SERUM PROTEINS IN RATS TREATED WITH TWO ORALLY DELIVERED LECTINS

Electrophoresis is a common technique to assess the serum or plasma proteins patterns associated with different physiological and pathological conditions in humans and animals. The aim of this study was to evaluate serum protein fractions and their changes induced by potato shoots and bean pods lectins’ administration to healthy Wistar rats. Both lectins induced an albumins (%) increase, accompanied by a slight decrease in α1 and α2 globulins. The most important change was recorded for β-globulins that significantly decreased in the case of both lectins administration. Serum protein electrophoresis demonstrated that the studied lectins, even orally delivered, survived proteolytic attack during gastrointestinal passage, were absorbed, and induced functional alterations of different organs.

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Cornelia FAFANEATA, Marian GHITA, Nicolae DOJANA, Aneta Pop 2013, COMPARATIVE ELECTROPHORESIS OF SERUM PROTEINS IN RATS TREATED WITH TWO ORALLY DELIVERED LECTINS. Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4, PRINT ISSN 2065-1299, 29-39.

COMPARATIVE EVALUATION OF SOMATIC CELLS LEVELS OF GOAT MILK FROM ALPINE AND CARPATHIAN BREEDS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Alina NĂSALEĂN, Iulia BALACI, Laura PARLAPAN and Laurenţiu OGNEAN

Milk is considered the most complete aliment, being abundant in high quality nutritive substances. The composition of goat milk, as well as its quality, differs between breeds and living area. Milk somatic cells are an indicator of hygienic quality as well as of the health of the mammary gland. The research aims the comparative analysis of milk quality depending on breed, as well as mammary gland health, goats being selected out of a group of 60 raised and fed in identical conditions in a private farm in Cluj County, considered clinically healthy being at the second lactation. For the determination there have been analyzed individual samples of raw milk harvested during July-September 2014 from Alpine (n=10) and Carpathian (n=10) breeds goats, using an automatic device for somatic cells count of Fossomatic type. Following the corroboration of data for 6 Alpine goats the number of somatic cells was over the maximum admitted limit (400000/ml milk), respectively 4 were under the limit. Fort the Carpathian breed 8 of them were between limits and only 2 went over this limit. The conclusion of this study highlights the acquired resistance of indigene breeds compared to imported ones.

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Năsaleăn A., Balaci I., Parlapan L., Ognean L. 2015, COMPARATIVE EVALUATION OF SOMATIC CELLS LEVELS OF GOAT MILK FROM ALPINE AND CARPATHIAN BREEDS. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 41-44.

COMPARATIVE RESEARCH ON THE USE OF CLASICAL ANTIBIOTIC AND ALTERNATIVE THERAPIES AGAINST BOVINE MASTITIS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Radu M. GIUPANĂ, Ioan S. GROZA, Constantin Gh. CERBU, Mihaela NICULAE, Krisztina RINDT, Marina SPINU

Bovine mastitis is a major problem of dairy animals despite the numerous preventive and therapeutic approaches. Given the increased antibiotic resistance of the involved bacterial strains, this research aimed to evaluate the efficacy of alternative therapy with honey and propolis in treating mastitis in cattle. The research was carried out on a group of 28 animals, aged 3 to 11 years, of Romanian Spotted and Red Holstein breeds. The investigations aimed the isolation and identification of bacteria involved in cases of clinical mastitis in cows, evaluation of their sensitivity/resistance to commonly used antibiotics, as well as the assessment of honey and propolis efficacy on bacteria isolated from mastitis cases of intensively managed cows. Main methods used were classical cultivation and Kirby-Bauer disk diffusion susceptibility test. Antibiotic resistant or highly resistant staphylococci were encountered in almost all milk samples. The comparative study regarding the use of various propolis tincture concentrations showed maximum efficacy for the 20% concentration, with decreasing effects for larger concentrations, which denied the hypothesis according to which increased concentrations produce increased effect. The efficacy of honey products depended upon concentration and bacterial strain, individualized tratment schemes being absolutely necessary.The results indicated that frequent and uncontrolled use of antibiotics against mastitis led to the development of multi- or total resistance to antibiotics, thus honey and propolis represented valuable therapeutical alternatives, especially in case of Staphylococcus. The obtained results are encouraging, mainly for the clinical use of propolis in therapy alone or in combination with antibiotics, after standardization of the method through in vivo studies and finding a method for diminishing the irritative effects of the propolis tincture.

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Giupană R. M., Groza I. S., Cerbu C. G., Niculae M., Rindt K., Spinu M. 2015, COMPARATIVE RESEARCH ON THE USE OF CLASICAL ANTIBIOTIC AND ALTERNATIVE THERAPIES AGAINST BOVINE MASTITIS. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 184-190.

COMPARATIVE STUDY BETWEEN THE ANTIBIORESISTANCE OF CAMPYLOBACTER JEJUNI AND CAMPYLOBACTER COLI

The spread of antibiotic resistances and the appearance of multiple-antibiotic-resistant pathogenic bacteria has been recognized by the WHO as a serious problem that complicates medical treatment of bacterial infections. In last years, when campylobacteriosis became the most frequently disease the antibioresistance of this microorganism represents a serious problem. Campylobacter’s antibioresistance was carried out through the determination of minimal inhibitory concentration using Sensititre system. This analysis were performed in panels dedicated for these microorganisms. There were tested 132 Campylobacter jejuni and Campylobacter coli strains previously isolated from chicken meat. The species of Campylobacter was perform according ISO 10272/2006.Of the 132 analyzed Campylobacter strains, 39 strains were susceptible to all antimicrobial substances tested, and 93 strains showed resistance to at least one antimicrobial agent; 4 strains were resistant to 6 antimicrobial substances. C. coli showed a higher degree of resistance than C. jejuni to all antimicrobial substances that were tested.

COMPARATIVE STUDY OF THE THORACOABDOMINAL SKELETON IN CHICKENS (GALLUS GALLUS) AND GEESE (ANSER ANSER)

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Ioana DUMITRU, Cristian MARTONOS, Bianca MATOSZ, Flaviu Tuns, Florin SILAGHI, Aurel DAMIAN

Although the predominant species found in poultry farming are galliforms and anseriforms there are few comparative studies referring to the skeletal morphology of these species. This study compares thoraco-abdominal component of these species in light of their different adaptation to the environment. The research was carried out in the Compared Anatomy laboratory of the Faculty of Veterinary Medicine of Cluj-Napoca. A total number of 6 skeletons, 3 chickens and 3 gooses were examined following comparative aspects of the anatomical structures relevant for this area. After examining the bones from the thoraco-abdominal region, differences as well as similarities between the two species of birds were observed. The thoracic cavity in chickens presented 7 pairs of ribs, of which 2 were asternal, while the same segment in geese contained a number of 9 ribs of which only 1 asternal. The sternum, presented 3 pneumatic holes in chickens and only one in geese. Also, differences were noted at the level of the sternal body, which was reduced in the chicken compared to the goose. On the lateral sides of the sternal body, the goose registers 7 or 8 costal indentations depending on the individual, compared to only 3 found in the chicken. The coxo-dorso-lumbosacral complex presented small differences between the two species. The iliac fossa in chickens is well defined, the pubis is oriented dorsally, slightly curved medially and the coccygeal vertebrae are 7 to 8 depending on the individual. In geese, the external iliac fossa is absent, the pubis is oriented ventrally, curved medially and the coccygeal vertebrae are 6, the pygostyle being fused to the last vertebrae.

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Ioana DUMITRU, Cristian MARTONOS, Bianca MATOSZ, Flaviu Tuns, Florin SILAGHI, Aurel DAMIAN 2013, COMPARATIVE STUDY OF THE THORACOABDOMINAL SKELETON IN CHICKENS (GALLUS GALLUS) AND GEESE (ANSER ANSER). Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4, PRINT ISSN 2065-1298, 25-28.

CONFIRMATORY METHOD OF RESORCYLIC ACID LACTONES IN URINE AND TISSUE BY LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY

This paper presents a liquid-chromatography negative ion electrospray tandem mass-spectrometry (LC-MS/MS) method to determine resorcylic acid lactones (RALs) in urine, muscle, fish, liver and kidney samples. The method comprises an extraction step followed by the clean-up of the samples in two steps using SPE cartridges: C18 Chromabond and Strata Amino from Phenomenex. The RALs were separated on a Pursuit C18 column. in isocratic mode with acetonitril/ 0.1% formic acid aqueous solution. The time for analysis was 12 minutes. A triple quadrupole mass spectrometric from Varian equipped with a ESI source in the multiple reaction monitoring was used for detection. The method was developed according to EU legislation and the parameters assessed were: selectivity, specificity, linearity, recovery, repeatability, within-laboratory reproducibility, decision limit (CC), and detection capability (CC), ruggedness, control chart, uncertainty of measurement. The validation was performed at 1μg/kg for each compound. CC and CC values were 0.16-0.79 micro g/kg and the range of mean recoveries were 72.2-119.7% depending of the compound. The relative standard deviation (RSD) of the measurements was below 20%.The method is used to analyze samples originating from the Romanian Residue Control Monitoring Program or private samples. The main purpose of this method is the detection, quantification and confirmation of 6 RAL’s in urine and tissue.

CONTRIBUTIONS OVER THE ANATOMY OF PRIMITIVE AORTA AND ITS MAIN BRANCHES IN SWINE

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Flaviu TUNS, Alina IURCUT, Ioana CHIRILEAN, Alexandra BLENDEA, Aurel DAMIAN

Previous studies performed in human and swine regarding the segment of ascending aorta and aortic arch identified several differences that referred to the presence and number of aortic collaterals in this region. Other studies mention the intraspecific differences in humans that refer to the way in which the aortic branches and even to their number. Due to the numerous similitudes of the cardiovascular system between humans and swine, we choose this specie to study the intraspecific variations regarding the aortic branches. The objective of this paper was to realize a comprehensive study of the swine aorta, in its initial part, namely the aortic arch, to highlight its systematization and to describe its branches and their count. To highlight the potential particularities found in the initial part of aorta we initially dissected the arteries and then injected them with latex mixed with red pigment to obtain a better view of the blood vessels. The process of latex injection was made by two ways: through the left ventricle and through the abdominal aorta. Following injection, the biological material was fixed in 2% formalin for 48 hours. Through dissection we observed that aorta presents several specie peculiarities in swine. We did not find any intraspecific differences regarding the primitive aorta or aortic arch. The particularities that were found are characteristic to the specie and refer to the presence of an aortic bulb with a calibre similar to that of the aortic arch; the presence of sigmoid valves that were well developed; the absence of the ascending aorta in swine. We also found a left subclavian artery more developed then the one from the right side.

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Flaviu TUNS, Alina IURCUT, Ioana CHIRILEAN, Alexandra BLENDEA, Aurel DAMIAN 2013, CONTRIBUTIONS OVER THE ANATOMY OF PRIMITIVE AORTA AND ITS MAIN BRANCHES IN SWINE. Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4, PRINT ISSN 2065-1302, 44-50.

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