EFFECT OF VARIOUS LEVELS OF DIETARY CALCIUM ON BLOOD CALCIUM CONCENTRATION AND HORMONAL STATUS IN WHITE CORNISH AND WHITE LEGHORN HENS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by EFFECT OF VARIOUS LEVELS OF DIETARY CALCIUM ON BLOOD CALCIUM CONCENTRATION AND HORMONAL STATUS IN WHITE CORNISH AND WHITE LEGHORN HENS

Two hen breeds, White Cornish (COR) and White Leghorn (LEG) 34-week-aged were used in this experiment. The basic diet of these hens contained 3.8% Ca. This diet was supplemented for an experimental period of 8 days with 0.0, 1.0 and 2.0 and, respectively, 3.0% calcium. During the experimental period the plasma blood levels of Ca, phosphorus, parathormone, calcitonin, vitamin D and estradiol were monitored. The results relive a significant increase of the blood plasma levels of Ca and phosphorus in both COR and LEG hens beginning form the 2% calcium dietary supplementation. Phosphorus levels increased too, but the Ca/P ratio indicated an imbalance for the calcium. In the same time it was found a significant decrease of the plasma level of parathormone in both 2% and 3% Ca supplemented CRG and LEG hens. Blood plasma level of calcitonin doesn’t follow a clear increasing in any experimental group, neither for CRH nor for LEG hens: statistical analyses between day one and 8th day of the experimental periods didn’t relive any statistical significant difference in any experimental group for this hormone. Plasma vitamin D concentrations followed a statistical significant (P<0.05) increase for both 2% and 3% calcium supplemented diets in both CRN and LEG hens. Estrogen levels were found significantly elevated in LEG hens vs. CRH hens, but the differences were not in relation to the blood calcium concentrations. In conclusion, blood calcium levels in hens influences the parathormone and vitamin D secretions but its excact influence on the calcitonin and estrogens is not clear. Further experiments are recommended to find the exact mechanisms of blood calcium regulation in birds.

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Claudia PREDA, C. BUDICA, N. DOJANA 2013, EFFECT OF VARIOUS LEVELS OF DIETARY CALCIUM ON BLOOD CALCIUM CONCENTRATION AND HORMONAL STATUS IN WHITE CORNISH AND WHITE LEGHORN HENS . Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4, PRINT ISSN 2065-1300, 34-43.

EFFECTS OF CIS-PLATINUM ON THE BIOCHEMICAL HOMEOSTASIS IN RATS NOTE I. INVESTIGATIONS ON THE HEPATIC DNA AND ON SERUM PROTEINS

Cytostatic chemotherapy induces changes in the homeostasis of the hepatic DNA and of serum proteins. Among the various drugs used in the chemotherapy the alkilating agents, antimetabolites, steroid hormones and antibiotics are better known. In the last decades there were discovered other new compounds such as platinum derivates which representia distinct class of compounds, having specific antitumoral action. Among platinum derivatives an essential effect has cis-platinum. Research on the cytostatic activity of cis-platinum implies the knowledge of the pharmacokinetic and biochemical effects. Experiments "in vivo" performed on Wistar strain rats pursued the action of the intraperitoneally injected cis-platinum on the hepatic deoxyribonucleic acid (DNA) biosynthesis and on serum proteins. The statistically processed analytical data revealed non-significant decreases of the hepatic DNA and of serum proteins concentrations. Electrophoretical fractions showed hypoalbuminemia and hyperglobulinemia. In case of globulin subfractions the decrease of alpha 1 - and alhpa 2 - and increase of beta - and yota - globulins was observed.

EFFECTS OF COENZYME Q10 ON SPERM VIABILITY DURING STORAGE OF BOAR SEMEN AT 17°C

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Laura PARLAPAN PÎNDARU, Mihai CENARIU, Emoke PALL, Ioan Ștefan GROZA

Currently, there is a growing interest on cryopreservation of boar semen even if the sperm membrane of swine shows high sensibility to this process. Previous studies have shown that the cryoconservation has an oxidative degradation effect, associated with the production of reactive oxygen species (ROS). ROS cause lipid peroxidation in sperm membranes and a variety of physical and chemical changes of sperm cells that predispose to DNA damage and apoptosis. The only alternative to counteract the effects of ROS is the addition of various antioxidants in lipid storage extenders. Starting from this premise, this work investigates Coenzyme Q10 effects on the viability of preserved boar semen during liquid storage. Semen was randomly divided into 5 groups and treated with different concentration of Coenzyme Q10 and storage at 17ºC for 5 days. The viability of semen was evaluated every day, using flow cytometer FACSCanto II (BD Biociencias) systems. The samples for FACS were labeled with Hoechst 33342, floresceinisothiocyanate conjugated with peanut agglutinin and propidium iodide. These experiments indicate that supplementation of Coenzyme Q10 to the semen extender can increase the sperm characteristics and prolong the survival of liquid storage semen, which may have potential benefits in reproductive biotechnology field.

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Pîndaru L. P., Cenariu M., Pall E., Groza I. Ş. 2015, EFFECTS OF COENZYME Q10 ON SPERM VIABILITY DURING STORAGE OF BOAR SEMEN AT 17°C. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 32-36.

EFFECTS OF THE PHOTOPERIODICITY ON THE REPRODUCTION IN SOW. II-EFFECTS ON THE ESTROUS CYCLE, PREGNANCY LENGTH AND TOTAL BORN PIGLETS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Costin BUDICA, Rosalie DOJANA, Laurent OGNEAN, Iuliana CODREANU, Nicolae DOJANA

In the frame of this paper it was researched the relationship between season and some reproductive parameters (oestrus length, pregnancy length and total born piglets) in primiparous and multiparous sows in terms of the photoperiodicity climate in Romania. The research was conducted on a crossbred Yorkshire sows (♀) × Landrace (♂) sow population and consisted of monitoring the oestrus duration, the gestation period and the number of total born piglets related to astronomical seasons: fall, winter, spring and summer. They were found seasonal influences characterized by a longer gestation length in autumn and spring seasons vs. summer and winter, with a difference of about one day between the seasons, and an annual average difference of about one day between primiparous and multiparous sows. Gestation length was inversely correlated with total born piglets. The oestrus length had an annual average of about 2.16 days in primiparous and 2.98 days in multiparous sows, with peak values in seasons of growing photoperiodicity and minimum values in seasons of decreasing photoperiodicity.

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Budica C., Dojana R., Ognean L., Codreanu I., Dojana N. 2015, EFFECTS OF THE PHOTOPERIODICITY ON THE REPRODUCTION IN SOW. II-EFFECTS ON THE ESTROUS CYCLE, PREGNANCY LENGTH AND TOTAL BORN PIGLETS. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 227-231.

EFFECTS OF THE PHOTOPERIODICITY ON THE REPRODUCTION IN SOW. II-EFFECTS ON THE REPRODUCTIVE HORMONAL SYSTEM

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Costin BUDICA , Rosalie DOJANA, Laurent OGNEAN, Gabriel COTOR, Nicolae DOJANA

The research was conducted on a breed of adult sow in different physiological states, originated from a northern EU area, during the period of adaptation in a temperate (Romanian) area. The animals were in different physiological status: gilts and sows, estrous, pregnancy or lactation. They were determined the blood plasma levels of main hormones involved in the reproductive function [17-estradiol, progesterone, follicle stimulating hormone (FSH), luteinizing hormone (LH) and melatonin] in the days of the solstices and equinoxes. Plasma levels of 17- estradiol for gilts and sows during the first day of the estrous showed minimal values in March, 20th, increased in June 21st, reaching a maximal values in September, 22nd, and then decreased again. The LH mean values (assayed in the first day of estrous period) were highest during the maximum photophase (June, 21st) and lowest in September, 22nd in gilts, while in sows, the higher levels of plasma LH were found during the day of spring equinox and summer solstice and the lowest levels in September, 22nd (as in gilts). Plasma progesterone of the 25-day-pregnant gilts and sows presented the highest values during the period of maximum scotophase (December, 21st), significantly higher by comparing to the other three analyzed photoperiods. Plasma progesterone of pregnant gilts was lower vs. pregnant sows for every analyzed photoperiod. The maximum amounts of melatonin blood values were found in December 21st, the lowest in June 21st and intermediate values in the two solstices. For all the assayed photoperiods, melatonin contents in midnight samplings were nearer to those taken at midday. Almost every time, the melatonin values in lactant sows were lower vs. in pregnant sows.

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Budica C., Dojana R., Ognean L., Cotor G., Dojana N. 2015, EFFECTS OF THE PHOTOPERIODICITY ON THE REPRODUCTION IN SOW. II-EFFECTS ON THE REPRODUCTIVE HORMONAL SYSTEM . Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 19-24.

ELECTROENCEPHALOGRAPHIC DIAGNOSIS IN DOGS SUFFERING FROM EPILEPSY OF UNKNOWN ORIGIN

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Gabriela – Dumitrita STANCIU, Mihai MUSTEATA, Mihaela ARMASU, Gheorghe SOLCAN

Epilepsy of unknown origin is incurable, but treatment in the form of constant medication can help control and prevent progression in the severity of the condition in many cases. If untreated, epilepsy of unknown origin can, in extreme cases, lead to cluster seizures and status epilepticus and death. The aims of this study were to describe a short time EEG recording (30 minutes) using Redding’s model to determine the diagnostic value of electroencephalographic recordings in dogs suffering from epilepsy of unknown origin. Electroencephalograms were performed on 24 dogs of mixed breeds suffering from epilepsy of unknown aetiology. Anaesthesia was induced with medetomidine hydrochloride and ketamine. EEGs were obtained via five subdermal needle electrodes. The EEG was recorded with sensitivity = 70

ELECTRORETINOGRAPHY: SELECTION OF PATIENTS AND PERFORMING THE TECHNIQUE

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIII, Issue 1
Written by Adina ARGASEALA, Lia ION, Gina GIRDAN, Ion Alin BIRTOIU, Jacqueline MOCANU, Iuliana IONASCU

Electroretinography (ERG) is an objective test that evaluates the retinal function. This paper wants to present to the veterinary Romanian scientific community the importance of using this diagnostic tool for clinicians dealing with blind patients. Candidates for ERG were selected from ophthalmological patients presented with blindness because it was difficult to establish a definitive diagnosis using only ophthalmological examination. All patients underwent general anesthesia. ERG was performed using the HMsERG unit, with preprogrammed included protocols. The protocols used in this study were Short protocol for the cataract surgery candidates and ISCEV protocol for the other patients. ERG confirmed the results obtained on chromatic pupillary light reflex evaluation. ERG proved useful in the following cases: diagnosing retinal inherited disorders, differentiating between retinal and postretinal causes of blindness, evaluation of retinal function in cataract surgery candidates. ERG objectively assesses the retinal function and should be used on a scale by practitioners dealing with blind patients.

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ARGASEALA A., ION L., GIRDAN G., BIRTOIU I.A., MOCANU J., IONASCU I. 2017, ELECTRORETINOGRAPHY: SELECTION OF PATIENTS AND PERFORMING THE TECHNIQUE. Scientific Works. Series C. Veterinary Medicine, Vol. LXIII, Issue 1, ISSN 2065-1295, 50-54.

EMERGING DISEASES ASSOCIATED WITH“NEW COMPANION ANIMALS”: REVIEW IN ZOONOSES TRANSMITTED BY REPTILES

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Ioana LUPESCU, Stelian BARAITAREANU

Several zoonoses, including rare human diseases, can be transmitted by primates, exotic rodents, lagomorphs and carnivores, marsupials, bats, fish, amphibians and reptiles which are held in households as companion animals. Over the past few years, the interest in wild animals as pets has increased and this interest can also be observed in Romania. The risk of owning wild animals is significant because over 70% of zoonotic emerging infections originate in wildlife. Pathogens can be transmitted to humans through direct contact (e.g. Salmonella spp., Klebsiella spp., Enterobacter spp., Aspergillus spp., Candida spp., Mites), puncture wounds (e.g. Aeromonas spp., Mycobacterium spp., Zygomycosis, Phycomycosis, Mucormycosis), ingestion (e.g. Salmonella spp., Aeromonas spp., Campylobacter spp., Gnathostomiasis) or inhalation (Mycobacterium spp., Aspergillus spp., Candida spp.). In this paper, we reviewed zoonoses and zoonotic agents that can be transmitted by reptiles. To identify pathogens frequently involved in zoonoses transmitted by reptiles, we studied official reports of WHO and scientific papers published in the last ten years. The following diseases were analysed: salmonellosis, tuberculosis (Mycobacterium marinum), campylobacteriosis, Q-fever, Baker-Rosenbach's erysipeloid, Edwardsiella tarda infection and Aeromonas infection. The numbers of pathogens that can be transmitted by exotic pets and the severity of diseases that these pathogens cause to humans and other animals can be high. However, reptiles weren’t involved in severe zoonoses outbreaks, and the probability of introducing a severe zoonosis in endemic regions seems to be low. Unfortunately, pet owners don’t take into consideration the diseases that their animals can transmit, they do not ask for specialists' recommendations and they ignore the preventive measures that should be taken. As a conclusion, the reptile keepers should consider preventive measures, such as: (1) rigorous personal hygiene after contact with an exotic animal; (2) the use of protective equipment, especially when handled animals are showing clinical signs of disease; (3) isolation and treatment of ill animals; (4) periodic cleaning and disinfecting of the accommodation cages.

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Lupescu I., Baraitareanu S. 2015, EMERGING DISEASES ASSOCIATED WITH“NEW COMPANION ANIMALS”: REVIEW IN ZOONOSES TRANSMITTED BY REPTILES. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 135-138.

ENDOSCOPICAL MONITORING OF THE MARE'S REPRODUCTIVE TRACT FOR ESTRUS AND OVULATION DETECTION

Detecting the right moment of estrus in witch ovulation occurs it is extremely important for mare's biotechnology reproductive management and especially for mare breeding soundness evaluation. Endoscopic examination of mare's reproductive tractus makes possible the visualization of the vagina, the external cervical ostium, the lumen of the uterine body and the horns up to the uterotubal junctions, in order to obtain a large amount of information related with mares reproductive status. The endoscopic reproductive examination was performed at the Faculty of Veterinary Medicine Bucharest, for 15 mares from different races, examined for a research study concerning the best ways to monitor the ovulation. The examination was performed with a Olympus GIF K2, a flexible endoscope.

ENVIROMENTAL PROTECTION OF BIODIVERSITY WITH IMPLICATION IN COMPARATIV ECOPATOLOGY

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Iudith IPATE, Alexandru BOGDAN, Constanța STRASSER, Simona IVANA, Horatiu STRASSER, Janos SEREGI, Nicolae IPATE, Gabriela DAVID, Janos TOSSENBERGER

United Nations (UN) has designated the period 2011-2020 as the "United Nations Decade dedicated to biodiversity". The main implications on health status in relation to biodiversity include: health and nutrition security, infectious diseases, medical science and resource development of drugs at social and psychological health and spiritual wellbeing. Bio-security of natural resources is a major need food as a sustainable economy must respect "offer" ecosystems. Requests exceeded global sustainable productivity of natural systems with over 50% of humanity consumes more natural resources since 2007. Food poisoning is a re-emerging health problem, according to reports of drug prevention and control the national and European of communicable diseases which shows an increase in the number of foodborne illnesses caused by microorganisms such as Salmonella, Campylobacter, Escherichia coli, parasites. Such re-emerging disease is caused by a complex of factors acting as a result of the rapid changes taking place globally, including demographic changes, new practices intensive animal husbandry, extensive proliferation of systems for preparing and distributing food, changing eating habits and so on lead to less common pathogen infection and rapid spread and the geographic scope of pathogens already recognized representative.

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Ipate I., Bogdan A., Strasser C., Ivana S., Strasser H., Seregi J., Ipate N., David G., Tossenberger J. 2015, ENVIROMENTAL PROTECTION OF BIODIVERSITY WITH IMPLICATION IN COMPARATIV ECOPATOLOGY. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 232-237.


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