INTRASPECIFIC ANATOMICAL ASPECTS OF CARDIAC ARTERIES (AA. CORONARIA) IN THE DOG

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Ioana CHIRILEAN

By injecting the coronary arteries in common breed dogs of various ages, we have underlined certain modifications concerning the path, the caliber and the structure of the cardiac vessels. In this paper we will refer exclusively to the anatomical aspects of these vessels. In young subjects, both the cardiac arterial trunks and their branches present in general well traced paths, without changes in the arterial walls. Compared to this situation, in aged animals, we have both anatomical modifications of vascular paths and of their lumen’s caliber, as well as the varicose aspect of their walls. These morphological aspects also imply alterations of the differentiated vascular perfusion of the myocardium, while facilitating clinical interpretation in canine cardiac pathology.

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Chirilean I. 2015, INTRASPECIFIC ANATOMICAL ASPECTS OF CARDIAC ARTERIES (AA. CORONARIA) IN THE DOG. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 24-28.

INVESTIGATION OF ANTIOXIDANT COMPOUNDS IN FLUOROTIC SHEEP

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 2
Written by Inci DOGAN, Handan MERT, Kivanc IRAK, Nihat MERT

Fluorosis, a condition which usually affects the formation of bone and teeth in human and animals, is an important health problem in Van and Agri provinces. This study was performed to determine the levels and the changes of antioxidant compounds in fluorotic sheep. 30 fluorotic sheep and 20 healthy Morkaraman sheep of 3-4 years old were used as living research materials. The diagnosis of fluorosis was confirmed by clinical examinations. The urine fluoride level was determined. Blood of all animals was taken from vena jugularis by appropriate techniques and analyzed for glutathione peroxidase (GPx), glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), sialic acid (SA) and lipid-bound sialic acid (LSA). The levels of these parameters in healthy and fluorotic group were: 1028-416.8 mU /ml, 23.23-50.16 mg/ dl, 9.25- 7.88 mU/ ml, 1.62-0.56 nmol/ ml, 51.19-46.33 mg/ dl and 9.77- 12.16 mg/dl, respectively. Urine fluoride (F) levels were 1.65 ppm in healthy and 23.84 ppm in fluorotic sheep groups. Statistical differences was found between the average values of healthy and fluorotic groups as p≤ 0.0001 in GPx and GSH, p≤0.001 in MDA, p≤0.05 in SA, p≤0.01 in LSA and p≤0.001 in urine F levels. No statistical differences were found in SOD levels. (p≥0.05) The results obtained in this study indicate that important changes were found in antioxidant systems of fluorotic sheeps.

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DOGAN I,, MERT H., IRAK K., MERT N. 2016, INVESTIGATION OF ANTIOXIDANT COMPOUNDS IN FLUOROTIC SHEEP. Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 2, ISSN 2065-1295, 23-26.

INVESTIGATION THE ECOLOGY AND DISTRIBUTION OF PASSERIFORMES POPULATION FROM SACALIN ISLAND - DANUBE DELTA

Danube Delta is the second largest river delta in Europe, after Volga Delta, and the best preserved on the continent. Sacalin Island is a strictly protected area of the Danube Delta, declared biosphere reserve since 1938. The impact of human regarding the use of the same territories or interest in eating the same food resources disturbed bird’s habitats. The aim of this study was to investigate the distribution and ecology of passerine birds (Passeriformes) on the Sacalin Island in relation with anthropic changes. Data were obtained through the establishment of a permanent ringing station in Sacalin Island, with the support of the Romanian Ornithological Central and Nos Oiseaux Institute in Switzerland. As a working methodology, observations and bird ringing were made throughout the years 2007-2013, and distribution and ecology were analyzed. A total number of 6619 Passeriformes were collected, belonging of 13 familia. Among Passeriformes collected from Sacalin Island, Sylvidae familiae was the most divers with 14 species, followed by Muscicapidae with 8 species, Fingilidae (5 species), Turdidae (4 species), Paridae (3 species), Paradoxornithidae and Motacillidae (2 species) and Emberizidae, Oriolidae, Corvidae, Laniidae, Troglodytidae, and Hirundinidae with 1 species respectively. The predominant number of birds was found in Muscicapidae familiae (36.51%), followed by Sylviidae (29.65%), Turdidae (13.05%), Paridae (8.68% ), Laniidae (6.78%), Fringiliidae (2.07%), Troglodytidae (0.98%), Motacilidae (0.60%), Paradoxomithidae (0.51%), Oriolidae (0.50%), Emberizidae (0.40%), Corvidae (0.18%) and Hirundinidae (0.05%). In conclusion, our data suggest that the Sacalin Island is an area with a wide diversity of bird’s fauna, uncovering the ecology and distribution of birds could greatly improve the knowledge of bird’s dynamics and behavior.

ISOLATION AND IDENTIFICATION OF PSEUDOMONAS AERUGINOSA STRAINS PRODUCING β-LACTAMASES (ESBL) AND CARBAPENEMASES (MBL) OF HUMAN ORIGIN

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Oana Alexandra CIOCAN, Mihai CARP CĂRARE, Andreea Paula COZMA, Cătălin CARP–CĂRARE, Cristina RÎMBU, Gabriela COMAN, Elena PETRARU, Carmen–Valentina PANZARU

The community of medical veterinarians and humans draw attention to the microorganisms with potential pathogens that are both common and multiresistant to the latest antibiotics. Bacteria common to both animals and people favor the cross transmission of these strains with a major public health risk. Pseudomonas aeruginosa, a motile, nonfermenting Gram-negative bacterium, is an important opportunistic animal and human pathogen that causes acute and chronic infections in immunocompromised patients. Pseudomonas aeruginosa has acquired several mechanisms of resistance to multiple groups of antibiotic agents. The isolation and identification of Ps. aeruginosa producing ESBL and MBL may often be challenging to microbiology laboratories, the level of expression of β-lactamases and MBL can affect the performance of phenotypic tests, and the lack of synergy might be due to a very high level of expression overcoming the effect of the inhibitors resulting in falsenegative results. Ps. aeruginosa from different purulent collections has an atypical appearance, even a specific pigment that is not present for all the strains. These investigations are part of a larger research study, aimed at highlighting the strains of Pseudomonas aeruginosa common resistance genes in humans and animals. In this study, 93 Ps. aeruginosa strains were collected from pediatric patients. In order to establish resistance, profiling samples were isolated and identified for the production of β- lactamase medium Brilliance ESBL AGAR (Oxoid) and for the production of MBL was tested Imipenem with EDTA.

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Ciocan O. A., Carp-Cărare M., Cozma A. P., Carp–Cărare C., Rîmbu C., Coman G., Petraru E., Panzaru C. V. 2015, ISOLATION AND IDENTIFICATION OF PSEUDOMONAS AERUGINOSA STRAINS PRODUCING β-LACTAMASES (ESBL) AND CARBAPENEMASES (MBL) OF HUMAN ORIGIN. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 254-257.

LEUCINE UROLITHIASIS IN A 3 WEEKS OLD MIXED GERMAN SHEPHERD PUPPY

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Laeish JUNKEE, Marian A. TAULESCU

Canine urolithiasis is a common disorder of the urinary tract, characterized by stones located anywhere within the urinary tract, which is mostly encountered in middle-aged to older dogs. Urolithiasis is influenced by familial, congenital and pathophysiological factors including urinary pH, dehydration, urinary infection, anatomical abnormalities and drug administration. A 3 weeks old mixed German Shepherd male puppy with no antecedent clinical signs (sudden death) was submitted to the Pathology Department for necropsy. The animal was suspected of bronchopneumonia ab ingestis due to milk aspiration. Necropsy, cytological and histological exams were performed. Grossly, a large amount of urine was found within the peritoneal cavity (uroperitoneum) secondary to urinary bladder rupture, severe bilateral hydronephrosis and hydroureter, and urethral obstruction with numerous large white to gray calculi varying in size from 2-10mm were identified. The cytological exam showed several large, white to yellow spheroids with radial concentric laminations consistent with leucine crystals. Histologically, the renal tubules were diffusely dilated and contained pale eosinophilic hyaline casts, sloughed necrotic epithelial cells and lamellated concretions of amphophilic radiating structures. A diagnosis of urethral obstruction due to leucine urolithiasis was made, and it was associated with hydronephrosis, hydroureter and urinary bladder rupture. To the best of the authors’ knowledge this is the first report of leucine urolithiasis in a dog in Romania.

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Junkee L., Taulescu M. A. 2015, LEUCINE UROLITHIASIS IN A 3 WEEKS OLD MIXED GERMAN SHEPHERD PUPPY. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 161-164.

LID SPLITTING AND POSTERIOR LAMELLAR CRYOTHERAPY FOR CONGENITAL DISTICHIASIS AND TRICHIASIS IN DOG

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Andra ENACHE, Pip BOYDELL, Iuliana IONAŞCU, Alexandru ŞONEA

Various surgical techniques have been proposed for treating distichiasis in dogs. A technique involving eyelid splitting and double freeze-thaw cryotherapy with anterior lamellar recession was evaluated. A 3 year old, female, Staffordshire bull terrier was referred for bilateral distichiasis. There were bilateral multiple distichiasis of the upper lids, more severe on the right lid with double row of cilia and two cilia on the lower lid. Under general anaesthesia, the eyelid margin was split at the gray line and a cryoprobe was used to freeze the posterior lamella. A double freeze-thaw technique was applied in both eyes. Anterior lamellar recession was performed to prevent postoperative entropion with trichiasis. The anterior and posterior lamellas were sutured with a 6/0 Vicryl suture. Bilateral upper eyelid edema was noted postoperatively. A month follow-up revealed increased bilateral granulation and depigmentation and the recurrence of one follicle on the right upper lid. Five months postoperatively there was no recurrence in the left eye but three cilia were detected in the right upper lid. The follicles have regrown due to incomplete destruction of the roots. Lid margin split with cryotherapy is an effective method for treating distichiasis but might require several attempts and increase in the cryotherapy time.

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Enache E., Boydell P., Ionaşcu I., Şonea A. 2015, LID SPLITTING AND POSTERIOR LAMELLAR CRYOTHERAPY FOR CONGENITAL DISTICHIASIS AND TRICHIASIS IN DOG. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 99-102.

MACROSCOPIC RESEARCH REGARDING THE MORPHOLOGY OF THE CORONARY ARTERY ON DOMESTIC PIG

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1
Written by Iulian DUMITRESCU, Gabriel PREDOI, Cristian BELU, Bogdan GEORGESCU, Petronela ROȘU, Florina DUMITRESCU

The importance of knowing the detailed morphology of organs in this species result from the fact that currently, the domestic pig is becoming increasingly used in xenotransplantation. Most investigators agree that pigs have the potential to be the prime candidates for organ donation. Pigs are plentiful, are quick to mature, breed well in captivity, have large litters, and have vital organs roughly comparable in size to those of humans. The study was carried out on a total of 15 specimens in which the hearts were dissected after insertion into the arteries of the contrast dye. It has found a relative morphometric equality between the two coronary arteries. Right ventricular wall was irrigated primarily by the branches of the common trunk of the right coronary artery but also by paraconal branches. The left was irrigated by division of paraconal and left circumflex branches.

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DUMITRESCU I., PREDOI G., BELU C., GEORGESCU B., ROȘU P., DUMITRESCU F. 2016, MACROSCOPIC RESEARCH REGARDING THE MORPHOLOGY OF THE CORONARY ARTERY ON DOMESTIC PIG. Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1, ISSN 2065-1295, 11-17.

MAJOR SALIVARY GLANDS TOPOGRAPHY IN RATS AND THEIR RELATION WITH THE SURROUNDING ANATOMICAL TISSUES

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 2
Written by Bianca MATOSZ, Cristian DEZDROBITU, Cristian MARTONOS, Vlad LUCA, Sidonia BOGDAN, Aurel DAMIAN

The structure of the salivary glands is different depending on the species and diet. The glandular secretion can be serous, mucous or mixed. Within the same order, for instance in rodents, there are dissimilarities between the major salivary glands, even if the diet is similar. In this study, we used five Wistar rats, originating from the University of Medicine and Pharmacy “Iuliu Hațieganu” biobase, in Cluj-Napoca. After inducing neuroleptanalgesia, the method of euthanasia was cervical dislocation and then a stratigraphic dissection was accomplished. We mention that these euthanasia methods are allowed by 2010/63/UE directive of the European Parliament and the Council from September 22nd of 2010, regarding animal protection used for scientific purposes. The external features were assessed and compared to published literature on other similar species. The macroscopic examination revealed that the major salivary glands in rats are similar to those from other species of mammals when referring to the general macroscopic aspect. The parotid gland is localised at the base of the auricular concha, without exceeding the outer ear base, extended distally in the ventral cervical region. The rats’ ventral extremity of the parotid gland faces and ends with a sharp angle, toward the scapular-humeral joint, covering the jugular gutter with this layout. Aboral border of the mandibular gland is covered by ventral extremity of the parotid gland, both mandibular glands being near the external jugular veins. As a location, the mandibular glands in rats reside in the sublaryngeal and subtracheal region. Sublingual glands are located orally from the rostral pole of the mandibular glands, maintaining in a certain way the same layout as the mandibular glands.

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MATOSZ B., DEZDROBITU C., MARTONOS C., LUCA V., BOGDAN S., DAMIAN A. 2016, MAJOR SALIVARY GLANDS TOPOGRAPHY IN RATS AND THEIR RELATION WITH THE SURROUNDING ANATOMICAL TISSUES. Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 2, ISSN 2065-1295, 38-43.

MALE GENITAL SYSTEM LESIONS IN DOGS DIAGNOSED BY CYTOLOGICAL EXAMINATION

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Georgeta DINESCU, Sorina NICOLA, Ioana Cristina FUNDĂȚIANU, Claudia CONSTANTINESCU, Emilia CIOBOTARU

Understanding the pathological processes occurring in the male genital system requires advanced knowledge about its morphofunctional features. Even though the lesions of the male genital system in the dog are not as common, they constantly occur in general practice often being regarded as challenging in terms of diagnosis, treatment and prognosis. This study aims to evaluate the epidemiology, the cytological features and the efficacy of the cytological examination in achieving a definitive diagnosis in male genital system lesions in dogs. This study was conducted over a 5 years period (2008-2012) in the Departament of Pathological Anatomy of the Faculty of Veterinary Medicine Bucharest and consists of 109 male dogs presenting genital lesions. The samples were obtained by fine needle aspiration, imprinting, scraping and surgical biopsy. The slides were prepared by squeezing and sliding techniques. For cytologically examined samples Romanowsky type stains were used: classic or quick MayGrünwald Giemsa and Diff-Quick.29 cases of testicular lesions were both cytologically and histologically examined. During these 5 years, a total of 1872 male dogs have been specifically examined and 109 (5.8%) presented genital lesions. Of the 109 dogs considered for the study, 104 (95.4%) had testicular lesions and 5 (4.6%) had penile lesions. The 104 testicular lesions were diagnosed as follows: 20 cases (19.2%) with cryptorchidism and testicular hypoplasia, 16 cases (15.4%) with testicular degeneration, 10 cases (9.6%) with orchitis, and 58 cases (55.7%) with testicular tumours: seminoma (n=15), Sertoli cell tumours (n=13), interstitial (Leydig) cell tumours (n=15), mixed testicular tumours (n=15). The diagnosed penile lesions included acute balanoposthitis (n=1), squamous cell carcinoma (n=1) and transmissible venereal tumours (n=3). In both cytologically and histologically examined cases, cytological diagnosis was confirmed by histological diagnosis in 90% of the cases. Diagnostic errors occurred in individuals presenting testicular tumours where cytological examination did not confirm histological findings; in these cases histological examination revealed mixed tumours.

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Dinescu G., Nicola S., Fundățianu I. C., Constantinescu C., Ciobotaru E. 2015, MALE GENITAL SYSTEM LESIONS IN DOGS DIAGNOSED BY CYTOLOGICAL EXAMINATION. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 142-145.

MARKET SURVEY CONCERNING ORGANIZATIONAL CONDITIONS OF VETERINARY PHARMACIES IN BUCHAREST

Currently, in Romania, retailing medicinal and other veterinary products is an important part of veterinarians’ activity. The study was conducted from March to September 2013 in 20 veterinary pharmacies in Bucharest. The analyzed issues were related to: location, size, connection to mandatory utilities, drugs regime, arrangement of medicinal products on the shelf, conditions of microclimate, holding the required documents, filing records, employed personnel. Of the 20 veterinary pharmacies analyzed, 15 were located at the basement of residential buildings and had separate access than the one of tenants, while the remaining 5 pharmacies were located in separate buildings. All veterinary pharmacies were connected to sewer, water and electricity, and 13 pharmacies were secured with anti-theft systems. None of the analyzed veterinary pharmacies had laboratory, which means that none of these pharmacies prepared medicines. Veterinary pharmacy’s warehouse was equipped with temperature and humidity insurance systems in 12 pharmacies. In 8 pharmacies, microclimate parameters were recorded in special registers. Veterinarians were employed in all studied pharmacies, while in 7 units the personnel includes also veterinary technicians. Lockers for the storage of substances included in Separanda and Venena lists were present in 16 pharmacies. The study of 20 veterinary pharmaceutical units in Bucharest showed that they largely respect the organizational conditions specified by law; however, there is a relatively small number of units fully complying with legislative requirements.

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