EPIDEMIOLOGICAL AND MORPHOLOGICAL CHARACTERISTICS OF CUTANEOUS ROUND CELL TUMORS DIAGNOSED USING ASPIRATIVE CYTOLOGY IN DOGS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIII, Issue 1
Written by Georgeta DINESCU, Raluca Ioana RIZAC, Valentina PETRE, Claudia Mariana CONSTANTINESCU, Patricia Valentina DOBRE, Andrei TĂNASE

This paper’s purpose is to identify the epidemiologic and morphologic characteristics of cutaneous round cell tumors diagnosed using aspirative cytology in dogs. The study was conducted over a period of five years on a total of 225 dogs, aiming predisposition to gender, age and lesion location, as well as identifying the cytomorphologic characteristics of the lesions. Cytology was performed using FNA, smears being displayed by spreading, air dried and Romanowsky stained. Of the 225 studied cases of cutaneous round cell tumors, 110 (49%) were histiocytic tumors, 96 (43%) were diagnosed as mast cell tumors, 10 (4%) were plasma cell tumors, 2 (1%) cutaneous lymphomas and 7 (3%) were extragenital transmissible venereal tumors (cutaneous). The difference between sexes was not significant, 51% of the affected animals being males and 49% females. Most tumors were localized on the limbs (46%), followed by the trunk (38%) and head (20%).The relevance of the cytological examination was maximum for the mast cell tumors, differential diagnostic problems being faced between histiocytic tumors and plasma cell tumors or transmissible venereal tumors. Proper evaluation of cell populations, identifying the specific elements and morphological features of each cell type are essential, increasing the value of cytopathological diagnosis in veterinary medicine practice.

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DINESCU G., RIZAC R.I., PETRE V., CONSTANTINESCU C.M., DOBRE P.V., TĂNASE A. 2017, EPIDEMIOLOGICAL AND MORPHOLOGICAL CHARACTERISTICS OF CUTANEOUS ROUND CELL TUMORS DIAGNOSED USING ASPIRATIVE CYTOLOGY IN DOGS. Scientific Works. Series C. Veterinary Medicine, Vol. LXIII, Issue 1, ISSN 2065-1295, 90-94.

EPIDEMIOLOGICAL STUDY OF THE INCIDENCE AND RISK ANALYSIS IN MAJOR DISEASES OF ANIMALS IN ROMANIA AND IN THE WORLD IN THE PERIOD 2007-2014

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Magda GONCIAROV, Cristin COMAN

Epidemiological dynamics in major animal diseases, suffered extensive changes since the year 2007 and was dominated by vesicular disease, foot and mouth disease and Sheep and goat pox, to the detriment of diseases such as classical swine fever or avian flu, which dominate global epidemiological picture in the beginning of the years 2000. This paper aims to highlight the dynamics of major diseases in the last decade, the factors that led to their reappearance in areas that were eradicated for a long time, and the impact of national programs accelerated eradication of diseases like equine infectious anemia, classical swine fever, and others.

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Gonciarov M., Coman C. 2015, EPIDEMIOLOGICAL STUDY OF THE INCIDENCE AND RISK ANALYSIS IN MAJOR DISEASES OF ANIMALS IN ROMANIA AND IN THE WORLD IN THE PERIOD 2007-2014. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 69-74.

EPIDURAL DELIVERY OF LIDOCAINE AND TRAMADOL TO CONTROL PAIN DURING OVARIOHISTERECTOMY IN THE BITCH

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Manuela PASCAL, Ruxandra COSTEA, Dorin ȚOGOE, Alexandru VIȚĂLARU, Alexandru DIACONESCU, Alin Ion BÎRȚOIU

Epidural anesthesia is a simple, safe and effective way to administer anesthesic and analgesic drugs for caudal abdominal surgeries in canines. The aim of the study was to compare the analgesic effect of lidocaine or lidocainetramadol association administered epiduraly during ovariohisterectomy in the bitch. 14 bitches, ASA status 2 to 4, were premedicated with midazolam 0,2 mg/kg, butorphanol 0,2 mg/kg, induced with propofol 5 mg/kg iv and mantained with isoflurane. For 7 bitches lidocaine 2 mg/kg was administered epiduraly (L batch), while for the other 7 lidocaine was associated v:v with tramadol (LT batch). Analgesia was monitored intraoperatory (pulse frequency, non invasive blood presure, muscle relaxation) and postoperatory for the first 4 hours using the Glasgow pain scale. Blood pressure remained constant for both groups. There was a deduction of volatile agents in both groups, the bitches being extubated soon after stopping the delivery of anesthesic gas. Glasgow pain score was higher for the L batch (10) ompared to the LT batch (5), showing a higher analgesic capacity for the lidocaine-tramadol association. There were no complications, adverse effects or technique related difficulties for the epidural anesthesia in the bitches included in this study. In conclusion, the association between lidocaine and tramadol for epidural anesthesia represents an efective, cheap and simple alternative for analgesia during ovariohisterectomy in the bitch.

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Pascal M., Costea R., Țogoe D., Vițălaru A., Diaconescu A., Bîrțoiu A. I. 2015, EPIDURAL DELIVERY OF LIDOCAINE AND TRAMADOL TO CONTROL PAIN DURING OVARIOHISTERECTOMY IN THE BITCH. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 168-171.

ESSENTIAL TRANSCRIPTION FACTORS FOR MOUSE BLASTOCYST STAGE EMBRYOS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Duygu MUTLUAY

Preimplantation is a process in which embryo is prepared for implantation to the wall of the uterus. This stage defines some morphological changes that occur in the first three days after fertilization during mouse development. It culminates in the generation of the blastocysts, which has a fluid-filled inner cavity and two distinct cell lineages. These two distinct cell lineages consist of trophectoderm (TE) and inner cell mass (ICM). While TE cells contribute to the placenta and the extraembryonic membranes and allow the blastocysts to implant in the mother’s uterine wall, the pluripotent inner cell mass gives rise to the fetus. Some transcription factors such as Cdx2 and Oct4 (Pou5f1) have been identified in the mouse blastocyst to generate TE and ICM. Cdx2 is specifically expressed in TE and suppresses the expression of Oct4. Oct4, a key regulator of pluripotency, is strongly expressed in ICM and is essential for early lineage segregation. To present the localization of this transcription factors, we flushed 2 cell stage embryos from the oviducts and cultured to late blastocyst stage in medium. Samples were fixed and immunostained with mouse anti-Cdx2, goat anti-Pou5f1, then images were assessed using a fluorescence microscope to show the localization and presence of Cdx2 and Oct4 by immunocytochemistry. In this work, we demonstrated and reviewed the current knowledge on the Cdx2 and Oct4 in the formation of TE and ICM that is important for understanding the mechanisms of mouse embryo development.

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Mutluay D. 2015, ESSENTIAL TRANSCRIPTION FACTORS FOR MOUSE BLASTOCYST STAGE EMBRYOS. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 35-40.

ESTIMATION OF OUTCOME OF UMBILICAL DISEASES BASED ON CLINICAL EXAMINATION: A RETROSPECTIVE STUDY INVOLVING 322 CALVES

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1
Written by Latif Emrah YANMAZ, Elif DOGAN, Zafer OKUMUS, Mahir KAYA, Armagan HAYIRLI

Ultrasonography is the most reliable examination in the diagnosis of umbilical diseases in calves. However, a large number of veterinarians are not capable of performing ultrasonographic examination. Therefore, the aim of this study was to assist practitioner in consideration of easily obtained clinical findings and possible outcome of the umbilical diseases according to clinical score. Medical records of owned 322 calves with different umbilical diseases (Omphalitis, umbilical abscess, urachal infection, umbilical hernia, omphalophlebitis, umbilical abscess+umbilical hernia, omphalitis+umbilical hernia) were reviewed. Clinical findings of each calf were pointed according to clinical scoring system. Surgery was performed in all types of umbilical diseases except for calves in group of omphalitis (medical treatment). The clinical score of animals was significantly changed according to umbilical disease. The highest clinical score was obtained in calves group of umbilical abscess+umbilical hernia (16.21±0.35). However, the calves in group of umbilical hernia (2.29±0.38) had the lowest clinical score. The clinical score with the highest specificity and sensitivity was >15 (sensitivity = 100 %, specificity = 91.5 %). A clinical score > 15 was associated with mortality rate of 98 % (95 % CI = 96-100). Mortality rates of omphalophlebitis, umbilical abscess+umbilical hernia and umbilical abscess were 16.7% (4/24), 15.2% (5/33) and 9.6% (7/73), respectively. Overall, 95% (306/322) of our calves were survived onemonth following surgery. In conclusion, the clinical score has an important role for outcome of the umbilical disease in calves.

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YANMAZ L.E., DOGAN E., OKUMUS Z., KAYA M., HAYIRLI A. 2016, ESTIMATION OF OUTCOME OF UMBILICAL DISEASES BASED ON CLINICAL EXAMINATION: A RETROSPECTIVE STUDY INVOLVING 322 CALVES. Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1, ISSN 2065-1295, 77-84.

ETIO-PATHOGENESIS OF SMALL RUMINANT LENTIVIRUS INFECTIONS: A CRITICAL REVIEW

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1
Written by Doina DANES, Dan ENACHE, Dragos COBZARIU, Stelian BARAITAREANU

RLVs are retroviruses belonging to the genus Lentivirus (subfamily Orthoretrovirinae). The earliest report of a disease whose pathological pattern suggest the SRLV infection was in Nederland, in 1862. Since then, several reports of clinical cases and scientific research, proved the wide dissemination of SRLV infections (Maedi-Visna in sheep and Caprine Arthritis-Encephalitis in goats) throughout all countries with large number of sheep and goats. In 1998, it was published a phylogenetic analysis of SRLV and it was proved the cross-species transmission of CAEV and MVV strains; moreover, in 2010, phylogenetic reconstructions supported the existence of SRLV cross-species transmission between domestic and wild small ruminants. SRLVs is a genetic continuum of lentiviral species (MVV, CAEV) in sheep and goats with evidence based of cross species transmission. The high genetic variability of SRLV, generate the classification of the viral genotypes into five groups and several subtypes, based on the phylogenetic analysis of two long genomic segments: the gag-pol segment (1.8 kb) and the pol segment (1.2 kb). Pathogenesis of lentiviral infections is the result of several particular factors, as the virus strain, the genetics of the host and the microenvironment. All this are influencing the tropism of lentivirus to a particular host animal or cell, tissue or organ. Till present, despite the huge and increasing speed in bio technics, the pathogenesis of SRLV infections, either in goat or in sheep, is not completely understood and the interaction of the host with those viruses is not fully known.

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DANES D., ENACHE D., COBZARIU D., BARAITAREANU S. 2016, ETIO-PATHOGENESIS OF SMALL RUMINANT LENTIVIRUS INFECTIONS: A CRITICAL REVIEW. Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1, ISSN 2065-1295, 59-64.

EVALUATION OF DIFFERENT TYPES OF BEER QUALITY AND CONSUMERS' SAFETY

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIII, Issue 1
Written by Oana Mărgărita GHIMPEŢEANU, Florin FURNARIS

In the context of high consumption of different types of beer and given the consumer demand regarding the food safety, the purpose of this study was represented by the quality control of these products using physicochemical methods. The data revealed information regarding pH value, alcohol content, carbon dioxide content, value of the original, real and present extract, energetic value and foam quality determination. The results showed an uniformity of data from lots of the same sort, which proved a core in applying of the quality management system. In conclusion, it can be said that products obtained in the studied unit meet quality requirements imposed by applicable standards and consumption of these products presents no risk of physicochemical nature.

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GHIMPEŢEANU O.M., FURNARIS F. 2017, EVALUATION OF DIFFERENT TYPES OF BEER QUALITY AND CONSUMERS' SAFETY. Scientific Works. Series C. Veterinary Medicine, Vol. LXIII, Issue 1, ISSN 2065-1295, 143-148.

EVALUATION OF RENAL VASCULAR RESISTANCE AND BLOOD PRESSURE IN DOGS WITH DIFFERENT RENAL DISEASES

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Radu CONSTANTINESCU, Victor CRIVINEANU, Gheorghe GORAN, Raluca Teodora NAE, Mario Darius CODREANU

The kidney is a well-vascularised organ and suitable to be evaluated by Doppler ultrasound, which is a non-invasive technique that can be used to estimate the renal vascular resistance by calculation the resistive index (RI) and pulsatility index (PI). RI and PI can be calculated from renal arteries, interlobular arteries, and arcuate arteries. In human patients, renal vascular resistance has been reported to be associated with the early hypertensive renal damage and also to correlate with the systemic blood pressure. The study was conducted over a two year period (December 2012- December 2014) in the Department of Internal Medicine of the Faculty of Veterinary Medicine Bucharest, on twenty eight dogs with different renal diseases. Significant differences were found between renal vascular resistance and red blood cell count, creatinine and blood urea nitrogen. Increased intrarenal vascular resistance may be associated with hypertension as a result of renal disease. The aim of this study was to assess renal vascular resistance in dogs with renal disease and the relation between renal RI and PI with systolic blood pressure in dogs with different renal diseases.

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Constantinescu R., Crivineanu V., Goran G., Nae R. T., Codreanu M. D. 2015, EVALUATION OF RENAL VASCULAR RESISTANCE AND BLOOD PRESSURE IN DOGS WITH DIFFERENT RENAL DISEASES. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 178-183.

EVOLUTION OF VETERINARY SERVICES IN ROMANIA IN THE PERIOD BEFORE ACCESSION TO THE EU AND AN TILL TODAY

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Magdalena GONCIAROV

Romania, since 2007, was included among European Union countries. In these circumstances, the Romanian state legislation passed through a series of changes that have always sought to align EU rules. These changes were applied including veterinary structures in Romania. For this reason, I had known organization of veterinary services in the European Union Member States for adoption by the Romanian state has a functional structure agreed by the European Union. In terms of veterinary organization in countries that joined the European Union for a longer period of time, the veterinary services have a stable organization that has as main purpose prevention and control of animal diseases (including zoonoses) and strict control of food, while in countries that recently joined the European Union, veterinary services mainly dealing with the implementation of laws and systems desired by the European Union and used therein.

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Magdalena GONCIAROV 2013, EVOLUTION OF VETERINARY SERVICES IN ROMANIA IN THE PERIOD BEFORE ACCESSION TO THE EU AND AN TILL TODAY. Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4, PRINT ISSN 2065-1315, 103-106.

EXPERIMENTAL INFECTION IN HONEYBEES USING HONEY WITH SPORES OF NOSEMA SP. (APIS/CERANAE)

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Agripina SAPCALIU A., MATEESCU C., SAVU V., RADOI I., MILITARU I.

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Agripina SAPCALIU A., MATEESCU C., SAVU V., RADOI I., MILITARU I. 2013, EXPERIMENTAL INFECTION IN HONEYBEES USING HONEY WITH SPORES OF NOSEMA SP. (APIS/CERANAE). Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4, PRINT ISSN 2065-1320, 123-128.

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