Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Laura PARLAPAN PÎNDARU, Mihai CENARIU, Emoke PALL, Ioan Ștefan GROZA
Currently, there is a growing interest on cryopreservation of boar semen even if the sperm membrane of swine shows high sensibility to this process. Previous studies have shown that the cryoconservation has an oxidative degradation effect, associated with the production of reactive oxygen species (ROS). ROS cause lipid peroxidation in sperm membranes and a variety of physical and chemical changes of sperm cells that predispose to DNA damage and apoptosis. The only alternative to counteract the effects of ROS is the addition of various antioxidants in lipid storage extenders. Starting from this premise, this work investigates Coenzyme Q10 effects on the viability of preserved boar semen during liquid storage. Semen was randomly divided into 5 groups and treated with different concentration of Coenzyme Q10 and storage at 17ºC for 5 days. The viability of semen was evaluated every day, using flow cytometer FACSCanto II (BD Biociencias) systems. The samples for FACS were labeled with Hoechst 33342, floresceinisothiocyanate conjugated with peanut agglutinin and propidium iodide. These experiments indicate that supplementation of Coenzyme Q10 to the semen extender can increase the sperm characteristics and prolong the survival of liquid storage semen, which may have potential benefits in reproductive biotechnology field.
[Read full article] [Citation]
