EFFECTS OF THE PHOTOPERIODICITY ON THE REPRODUCTION IN SOW. II-EFFECTS ON THE ESTROUS CYCLE, PREGNANCY LENGTH AND TOTAL BORN PIGLETS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Costin BUDICA, Rosalie DOJANA, Laurent OGNEAN, Iuliana CODREANU, Nicolae DOJANA

In the frame of this paper it was researched the relationship between season and some reproductive parameters (oestrus length, pregnancy length and total born piglets) in primiparous and multiparous sows in terms of the photoperiodicity climate in Romania. The research was conducted on a crossbred Yorkshire sows (♀) × Landrace (♂) sow population and consisted of monitoring the oestrus duration, the gestation period and the number of total born piglets related to astronomical seasons: fall, winter, spring and summer. They were found seasonal influences characterized by a longer gestation length in autumn and spring seasons vs. summer and winter, with a difference of about one day between the seasons, and an annual average difference of about one day between primiparous and multiparous sows. Gestation length was inversely correlated with total born piglets. The oestrus length had an annual average of about 2.16 days in primiparous and 2.98 days in multiparous sows, with peak values in seasons of growing photoperiodicity and minimum values in seasons of decreasing photoperiodicity.

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Budica C., Dojana R., Ognean L., Codreanu I., Dojana N. 2015, EFFECTS OF THE PHOTOPERIODICITY ON THE REPRODUCTION IN SOW. II-EFFECTS ON THE ESTROUS CYCLE, PREGNANCY LENGTH AND TOTAL BORN PIGLETS. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 227-231.

EFFECTS OF THE PHOTOPERIODICITY ON THE REPRODUCTION IN SOW. II-EFFECTS ON THE REPRODUCTIVE HORMONAL SYSTEM

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Costin BUDICA , Rosalie DOJANA, Laurent OGNEAN, Gabriel COTOR, Nicolae DOJANA

The research was conducted on a breed of adult sow in different physiological states, originated from a northern EU area, during the period of adaptation in a temperate (Romanian) area. The animals were in different physiological status: gilts and sows, estrous, pregnancy or lactation. They were determined the blood plasma levels of main hormones involved in the reproductive function [17-estradiol, progesterone, follicle stimulating hormone (FSH), luteinizing hormone (LH) and melatonin] in the days of the solstices and equinoxes. Plasma levels of 17- estradiol for gilts and sows during the first day of the estrous showed minimal values in March, 20th, increased in June 21st, reaching a maximal values in September, 22nd, and then decreased again. The LH mean values (assayed in the first day of estrous period) were highest during the maximum photophase (June, 21st) and lowest in September, 22nd in gilts, while in sows, the higher levels of plasma LH were found during the day of spring equinox and summer solstice and the lowest levels in September, 22nd (as in gilts). Plasma progesterone of the 25-day-pregnant gilts and sows presented the highest values during the period of maximum scotophase (December, 21st), significantly higher by comparing to the other three analyzed photoperiods. Plasma progesterone of pregnant gilts was lower vs. pregnant sows for every analyzed photoperiod. The maximum amounts of melatonin blood values were found in December 21st, the lowest in June 21st and intermediate values in the two solstices. For all the assayed photoperiods, melatonin contents in midnight samplings were nearer to those taken at midday. Almost every time, the melatonin values in lactant sows were lower vs. in pregnant sows.

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Budica C., Dojana R., Ognean L., Cotor G., Dojana N. 2015, EFFECTS OF THE PHOTOPERIODICITY ON THE REPRODUCTION IN SOW. II-EFFECTS ON THE REPRODUCTIVE HORMONAL SYSTEM . Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 19-24.

ELECTROENCEPHALOGRAPHIC DIAGNOSIS IN DOGS SUFFERING FROM EPILEPSY OF UNKNOWN ORIGIN

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Gabriela – Dumitrita STANCIU, Mihai MUSTEATA, Mihaela ARMASU, Gheorghe SOLCAN

Epilepsy of unknown origin is incurable, but treatment in the form of constant medication can help control and prevent progression in the severity of the condition in many cases. If untreated, epilepsy of unknown origin can, in extreme cases, lead to cluster seizures and status epilepticus and death. The aims of this study were to describe a short time EEG recording (30 minutes) using Redding’s model to determine the diagnostic value of electroencephalographic recordings in dogs suffering from epilepsy of unknown origin. Electroencephalograms were performed on 24 dogs of mixed breeds suffering from epilepsy of unknown aetiology. Anaesthesia was induced with medetomidine hydrochloride and ketamine. EEGs were obtained via five subdermal needle electrodes. The EEG was recorded with sensitivity = 70

EMERGING DISEASES ASSOCIATED WITH“NEW COMPANION ANIMALS”: REVIEW IN ZOONOSES TRANSMITTED BY REPTILES

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Ioana LUPESCU, Stelian BARAITAREANU

Several zoonoses, including rare human diseases, can be transmitted by primates, exotic rodents, lagomorphs and carnivores, marsupials, bats, fish, amphibians and reptiles which are held in households as companion animals. Over the past few years, the interest in wild animals as pets has increased and this interest can also be observed in Romania. The risk of owning wild animals is significant because over 70% of zoonotic emerging infections originate in wildlife. Pathogens can be transmitted to humans through direct contact (e.g. Salmonella spp., Klebsiella spp., Enterobacter spp., Aspergillus spp., Candida spp., Mites), puncture wounds (e.g. Aeromonas spp., Mycobacterium spp., Zygomycosis, Phycomycosis, Mucormycosis), ingestion (e.g. Salmonella spp., Aeromonas spp., Campylobacter spp., Gnathostomiasis) or inhalation (Mycobacterium spp., Aspergillus spp., Candida spp.). In this paper, we reviewed zoonoses and zoonotic agents that can be transmitted by reptiles. To identify pathogens frequently involved in zoonoses transmitted by reptiles, we studied official reports of WHO and scientific papers published in the last ten years. The following diseases were analysed: salmonellosis, tuberculosis (Mycobacterium marinum), campylobacteriosis, Q-fever, Baker-Rosenbach's erysipeloid, Edwardsiella tarda infection and Aeromonas infection. The numbers of pathogens that can be transmitted by exotic pets and the severity of diseases that these pathogens cause to humans and other animals can be high. However, reptiles weren’t involved in severe zoonoses outbreaks, and the probability of introducing a severe zoonosis in endemic regions seems to be low. Unfortunately, pet owners don’t take into consideration the diseases that their animals can transmit, they do not ask for specialists' recommendations and they ignore the preventive measures that should be taken. As a conclusion, the reptile keepers should consider preventive measures, such as: (1) rigorous personal hygiene after contact with an exotic animal; (2) the use of protective equipment, especially when handled animals are showing clinical signs of disease; (3) isolation and treatment of ill animals; (4) periodic cleaning and disinfecting of the accommodation cages.

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Lupescu I., Baraitareanu S. 2015, EMERGING DISEASES ASSOCIATED WITH“NEW COMPANION ANIMALS”: REVIEW IN ZOONOSES TRANSMITTED BY REPTILES. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 135-138.

ENDOSCOPICAL MONITORING OF THE MARE'S REPRODUCTIVE TRACT FOR ESTRUS AND OVULATION DETECTION

Detecting the right moment of estrus in witch ovulation occurs it is extremely important for mare's biotechnology reproductive management and especially for mare breeding soundness evaluation. Endoscopic examination of mare's reproductive tractus makes possible the visualization of the vagina, the external cervical ostium, the lumen of the uterine body and the horns up to the uterotubal junctions, in order to obtain a large amount of information related with mares reproductive status. The endoscopic reproductive examination was performed at the Faculty of Veterinary Medicine Bucharest, for 15 mares from different races, examined for a research study concerning the best ways to monitor the ovulation. The examination was performed with a Olympus GIF K2, a flexible endoscope.

ENVIROMENTAL PROTECTION OF BIODIVERSITY WITH IMPLICATION IN COMPARATIV ECOPATOLOGY

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Iudith IPATE, Alexandru BOGDAN, Constanța STRASSER, Simona IVANA, Horatiu STRASSER, Janos SEREGI, Nicolae IPATE, Gabriela DAVID, Janos TOSSENBERGER

United Nations (UN) has designated the period 2011-2020 as the "United Nations Decade dedicated to biodiversity". The main implications on health status in relation to biodiversity include: health and nutrition security, infectious diseases, medical science and resource development of drugs at social and psychological health and spiritual wellbeing. Bio-security of natural resources is a major need food as a sustainable economy must respect "offer" ecosystems. Requests exceeded global sustainable productivity of natural systems with over 50% of humanity consumes more natural resources since 2007. Food poisoning is a re-emerging health problem, according to reports of drug prevention and control the national and European of communicable diseases which shows an increase in the number of foodborne illnesses caused by microorganisms such as Salmonella, Campylobacter, Escherichia coli, parasites. Such re-emerging disease is caused by a complex of factors acting as a result of the rapid changes taking place globally, including demographic changes, new practices intensive animal husbandry, extensive proliferation of systems for preparing and distributing food, changing eating habits and so on lead to less common pathogen infection and rapid spread and the geographic scope of pathogens already recognized representative.

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Ipate I., Bogdan A., Strasser C., Ivana S., Strasser H., Seregi J., Ipate N., David G., Tossenberger J. 2015, ENVIROMENTAL PROTECTION OF BIODIVERSITY WITH IMPLICATION IN COMPARATIV ECOPATOLOGY. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 232-237.

EPIDEMIOLOGICAL STUDY OF THE INCIDENCE AND RISK ANALYSIS IN MAJOR DISEASES OF ANIMALS IN ROMANIA AND IN THE WORLD IN THE PERIOD 2007-2014

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Magda GONCIAROV, Cristin COMAN

Epidemiological dynamics in major animal diseases, suffered extensive changes since the year 2007 and was dominated by vesicular disease, foot and mouth disease and Sheep and goat pox, to the detriment of diseases such as classical swine fever or avian flu, which dominate global epidemiological picture in the beginning of the years 2000. This paper aims to highlight the dynamics of major diseases in the last decade, the factors that led to their reappearance in areas that were eradicated for a long time, and the impact of national programs accelerated eradication of diseases like equine infectious anemia, classical swine fever, and others.

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Gonciarov M., Coman C. 2015, EPIDEMIOLOGICAL STUDY OF THE INCIDENCE AND RISK ANALYSIS IN MAJOR DISEASES OF ANIMALS IN ROMANIA AND IN THE WORLD IN THE PERIOD 2007-2014. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 69-74.

EPIDURAL DELIVERY OF LIDOCAINE AND TRAMADOL TO CONTROL PAIN DURING OVARIOHISTERECTOMY IN THE BITCH

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Manuela PASCAL, Ruxandra COSTEA, Dorin ȚOGOE, Alexandru VIȚĂLARU, Alexandru DIACONESCU, Alin Ion BÎRȚOIU

Epidural anesthesia is a simple, safe and effective way to administer anesthesic and analgesic drugs for caudal abdominal surgeries in canines. The aim of the study was to compare the analgesic effect of lidocaine or lidocainetramadol association administered epiduraly during ovariohisterectomy in the bitch. 14 bitches, ASA status 2 to 4, were premedicated with midazolam 0,2 mg/kg, butorphanol 0,2 mg/kg, induced with propofol 5 mg/kg iv and mantained with isoflurane. For 7 bitches lidocaine 2 mg/kg was administered epiduraly (L batch), while for the other 7 lidocaine was associated v:v with tramadol (LT batch). Analgesia was monitored intraoperatory (pulse frequency, non invasive blood presure, muscle relaxation) and postoperatory for the first 4 hours using the Glasgow pain scale. Blood pressure remained constant for both groups. There was a deduction of volatile agents in both groups, the bitches being extubated soon after stopping the delivery of anesthesic gas. Glasgow pain score was higher for the L batch (10) ompared to the LT batch (5), showing a higher analgesic capacity for the lidocaine-tramadol association. There were no complications, adverse effects or technique related difficulties for the epidural anesthesia in the bitches included in this study. In conclusion, the association between lidocaine and tramadol for epidural anesthesia represents an efective, cheap and simple alternative for analgesia during ovariohisterectomy in the bitch.

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Pascal M., Costea R., Țogoe D., Vițălaru A., Diaconescu A., Bîrțoiu A. I. 2015, EPIDURAL DELIVERY OF LIDOCAINE AND TRAMADOL TO CONTROL PAIN DURING OVARIOHISTERECTOMY IN THE BITCH. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 168-171.

ESSENTIAL TRANSCRIPTION FACTORS FOR MOUSE BLASTOCYST STAGE EMBRYOS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Duygu MUTLUAY

Preimplantation is a process in which embryo is prepared for implantation to the wall of the uterus. This stage defines some morphological changes that occur in the first three days after fertilization during mouse development. It culminates in the generation of the blastocysts, which has a fluid-filled inner cavity and two distinct cell lineages. These two distinct cell lineages consist of trophectoderm (TE) and inner cell mass (ICM). While TE cells contribute to the placenta and the extraembryonic membranes and allow the blastocysts to implant in the mother’s uterine wall, the pluripotent inner cell mass gives rise to the fetus. Some transcription factors such as Cdx2 and Oct4 (Pou5f1) have been identified in the mouse blastocyst to generate TE and ICM. Cdx2 is specifically expressed in TE and suppresses the expression of Oct4. Oct4, a key regulator of pluripotency, is strongly expressed in ICM and is essential for early lineage segregation. To present the localization of this transcription factors, we flushed 2 cell stage embryos from the oviducts and cultured to late blastocyst stage in medium. Samples were fixed and immunostained with mouse anti-Cdx2, goat anti-Pou5f1, then images were assessed using a fluorescence microscope to show the localization and presence of Cdx2 and Oct4 by immunocytochemistry. In this work, we demonstrated and reviewed the current knowledge on the Cdx2 and Oct4 in the formation of TE and ICM that is important for understanding the mechanisms of mouse embryo development.

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Mutluay D. 2015, ESSENTIAL TRANSCRIPTION FACTORS FOR MOUSE BLASTOCYST STAGE EMBRYOS. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 35-40.

ESTIMATION OF OUTCOME OF UMBILICAL DISEASES BASED ON CLINICAL EXAMINATION: A RETROSPECTIVE STUDY INVOLVING 322 CALVES

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1
Written by Latif Emrah YANMAZ, Elif DOGAN, Zafer OKUMUS, Mahir KAYA, Armagan HAYIRLI

Ultrasonography is the most reliable examination in the diagnosis of umbilical diseases in calves. However, a large number of veterinarians are not capable of performing ultrasonographic examination. Therefore, the aim of this study was to assist practitioner in consideration of easily obtained clinical findings and possible outcome of the umbilical diseases according to clinical score. Medical records of owned 322 calves with different umbilical diseases (Omphalitis, umbilical abscess, urachal infection, umbilical hernia, omphalophlebitis, umbilical abscess+umbilical hernia, omphalitis+umbilical hernia) were reviewed. Clinical findings of each calf were pointed according to clinical scoring system. Surgery was performed in all types of umbilical diseases except for calves in group of omphalitis (medical treatment). The clinical score of animals was significantly changed according to umbilical disease. The highest clinical score was obtained in calves group of umbilical abscess+umbilical hernia (16.21±0.35). However, the calves in group of umbilical hernia (2.29±0.38) had the lowest clinical score. The clinical score with the highest specificity and sensitivity was >15 (sensitivity = 100 %, specificity = 91.5 %). A clinical score > 15 was associated with mortality rate of 98 % (95 % CI = 96-100). Mortality rates of omphalophlebitis, umbilical abscess+umbilical hernia and umbilical abscess were 16.7% (4/24), 15.2% (5/33) and 9.6% (7/73), respectively. Overall, 95% (306/322) of our calves were survived onemonth following surgery. In conclusion, the clinical score has an important role for outcome of the umbilical disease in calves.

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YANMAZ L.E., DOGAN E., OKUMUS Z., KAYA M., HAYIRLI A. 2016, ESTIMATION OF OUTCOME OF UMBILICAL DISEASES BASED ON CLINICAL EXAMINATION: A RETROSPECTIVE STUDY INVOLVING 322 CALVES. Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1, ISSN 2065-1295, 77-84.


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