ANATOMOCLINICAL OBSERVATIONS IN REOVIROSIS OF BROILERS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Oana Cătălina PETREC, Iulia BUCUR, FLUERAȘU L., Ionica IANCU

Avian reovirus infection is prevalent in intensive poultry farming, especially in broilers, wich evolve with many anatomoclinical forms. The researches was carried out in a flock of 10500 broilers,from Cobb hybrid. This flock was monitored until the age of 41 days, by clinical and anatomopathological exams, performed biweekly. The results were processed and graphically presented. The tests performed were identified following anatomoclinical forms of reovirosis: arthritis- tenosynovitis, ascites, hidropericard, proventriculus inflammation, catarrhal enteritis and necrosis of the femoral head uni and bilateral. Arthritis- tenosynovitis appeared at the age of 19 days maintaining in a relatively constant frequency until the end of the experiment. Ascites occurred at the age of 26 days with a frequency of between 12.5% and 20%. The hidropericard appeared at the age of 26 days, with a frequency between 12.5% and 42.85%. Catarrhal enteritis was reported at the age of 12 days, with a maximum frequency of 57.14%. Femoral head necrosis was signaled from the age of six days in both forms(unilateral and bilateral). Unilateral necrosis had a frequency of 42.85% at the age of 41 days and bilateral had necrosis has the frequency of 71.42% at the age of 21 days. The results obtained showed reovirosis evolution in broilers, in several clinical forms, confirmation of the disease was demonstrated by the polymerase chain reactionreverse transcriptase.

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Petrec O. C., Bucur I., Fluerașu L., Iancu I. 2015, ANATOMOCLINICAL OBSERVATIONS IN REOVIROSIS OF BROILERS. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 176-177.

ANTIOXIDANT ACTIVITY OF POLYPHENOLS EXTRACTED FROM HAWTHORN AND DOG-ROSE FRUITS ON LINOLEIC ACID EMULSION MODEL SYSTEM COMPARED TO BHA SYNTHETIC ANTIOXIDANT

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1
Written by Nicoleta Corina PREDESCU, Valentin NICORESCU, Carmen PETCU, Georgeta STEFAN, Camelia PAPUC

The ethanolic extracts of hawthorn (Crataegus monogyna) and dog-rose (Rosa canina) were found to contain polyphenols with antioxidant activity. The aim of this study was to assess the antioxidant properties of hawthorn and dog-rose polyphenols in linoleic acid emulsion model system, comparatively with synthetic antioxidant BHA. Polyphenols and BHA were incorporated in a linoleic acid emulsion at the final concentration of 100 ppm and then incubated at 80°C for 7 days. For determination of the progress of oxidation processes, primary and secondary peroxidation products levels were evaluated at every 24 hours. Hawthorn and dog-rose polyphenols inhibited the formation of lipid hydroperoxides, conjugated dienes and thiobarbituric acid reactive substances. The protective effect of hawthorn and dog-rose polyphenols was superior to the one of BHA synthetic antioxidant.

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PREDESCU N.C., NICORESCU V., PETCU C., STEFAN G., PAPUC C. 2016, ANTIOXIDANT ACTIVITY OF POLYPHENOLS EXTRACTED FROM HAWTHORN AND DOG-ROSE FRUITS ON LINOLEIC ACID EMULSION MODEL SYSTEM COMPARED TO BHA SYNTHETIC ANTIOXIDANT. Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1, ISSN 2065-1295, 32-35.

ANTIOXIDANT ACTIVITY OF SUNFLOWER AND MEADOW HONEY

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Corina PREDESCU, Camelia PAPUC, Valentin NICORESCU

Honey is a natural food produced by bees from the nectar of flowers and is a mixture of carbohydrates, amino acids, enzymes, vitamins and many bioactive compounds. Due to its special composition, honey is a functional food with antibacterial, anti-inflammatory and antioxidant properties. Studies have shown that honey’s properties depend mainly on the vegetable source from which it is obtained, but also on the processing type and storage. This research aims to evaluate the antioxidant capacity of sunflower honey compared to meadow honey by assessing total phenolic content, total flavonoid content, total antioxidant activity, free radicals scavenging activity and reducing power. For the determination of total phenols and flavonoids, antioxidant capacity and reducing power, spectrophotometric methods were used. To assess free radicals scavenging activity, chemiluminometric methods were used. Meadow honey showed the highest concentration of polyphenols (143.29 ± 9.12 mg GAE / kg) and flavonoids (118.09±8.84 mg CE / kg). DPPH radical scavenging capacity was higher for sunflower honey (78.32 ± 5.11%) compared to meadow honey (45.12 ± 3.26%). The two honey types showed capacity to scavenge superoxide anion and singlet oxygen, with an inhibition rate of over 50%. In conclusion, sunflower honey and meadow honey presented important concentrations of polyphenols and flavonoids and the results suggest a relationship between honey type and total polyphenols and free radicals scavenging activity

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Predescu C., Papuc C., Nicorescu V. 2015, ANTIOXIDANT ACTIVITY OF SUNFLOWER AND MEADOW HONEY. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 45-50.

ARTIFICIALLY FORCED FERTILITY IN DAIRY CATTLE EMBRYO TRANSFER

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Marcel Theodor PARASCHIVESCU, George Florea TOBĂ, Marcel PARASCHIVESCU, Mariana Sandu

Forced increased fertility in cattle can be obtained by multiple ovulation embryo transfer (MOET). This paper presents results of a trial to shorten interval between two pollyovulation treatments to the term determined by the natural estrus shown by donors after the previous embryos’ collection. Pollyovulation was provoked using FSH. Experiment started with 4 Romanian Black and White heifers, 17 month aged, but only two of them answered to the treatment for estrus synchronization. Both these heifers have shown heat in the same day, at 11 days after uterine washing. A total number of 27 yellow bodies were counted (14 units in one heifer and 13 in the other), but only 12 embryos were collected (11 embryos from the first heifer and 1embryo from the second). There is no explanation of the fact. The resulted interval between pollyovulation treatments was 53 days, 7 days less than the 60 days interval recommended by literature. More interesting was the presence of a total 26 atretic follicles in the 4 days (diestrus state) of embryo collection. They must pertain to the first wave of follicles starting maturation after prostaglandin injection given at embryo collection to prevent pregnancy. The fact suggests the possibility to stimulate these waves of follicles to have shorter interval between pollyovulation treatments. It is counted that the interval could be shortened up to 25 days. If that is possible MOET could be applied in heifers to increase selection precision of mother of sire dams by progeny testing them. New idea is to use MOET in dams of known genetic merit to discover better compatibility of parental pairs to obtaining the wanted type of the breed. Experiments are needed also to establish if more frequent uterine washings cause or don’t cause alteration of uterine mucosa functions.

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Paraschivescu M. T., Tobă G. F., Paraschivescu M., Sandu M. 2015, ARTIFICIALLY FORCED FERTILITY IN DAIRY CATTLE EMBRYO TRANSFER. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 255-259.

ASPECTS CONCERNING TAXIDERMY OF THE HEAD IN DEER

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Călin Csaba POP, Melania Ioana CRIȘAN, Aurel DAMIAN

Taxidermy is the art of dissecting and preserving animals as natural as possible, in order to exhibit them in natural science museums and in individual collections as trophies of the collectors or as decorating objects. In naturalization, minor surgical procedures for changing the mimics of the face are necessary in order to increase the value of the exhibits. The aim of the current work was to improve the technique of taxidermy in order to create valuable exhibits. Based on the bibliographic research and analysing the already existing pieces from different natural science museums and private collections, we noticed that technical improvements, especially of the facial region, were required in naturalizing the trophies coming from large mammals. The study was conducted on two deer heads. The naturalization was obtained by dissecting and removing the skin, excepting the ears, eye lids and oral cavity, where special procedures were required. The used method was systematic skinning. Tanning was made with eulan which insures a high flexibility and durability of the skin. The two prepared exhibits had an increased storage rate because there was no source of food for insects that could damage the material. With the improvement of naturalization technique of the exhibit, its storage life was also increasing. Application of new techniques of conservation and the use of new materials available in taxidermy, offers a long-term economic value to the exhibits in terms of structure and preservation of tissues.

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Csaba Pop C., Crișan M. I., Damian A. 2015, ASPECTS CONCERNING TAXIDERMY OF THE HEAD IN DEER. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 13-15.

ASSESSMENT OF MESENCHYMAL STEM CELLS EFFECTS ON DENDRITIC CELLS MATURATION

Introduction. Mesenchymal stem cells (MSCs) are adult stem cells (Pittenger et al., 1999, Zhang et al., 2009) able of self-renewal, with low immunogenicity and immunoregulatory property (Jorgensen et al, 2003, English et al., 2008, Zhang et al., 2009). Dendritic cells (DCs) present in the bone marrow play a crucial role in the instruction of adaptive immunity (Nauta et al., 2006, Zhi-Gang et al., 2012) DC have the unique capacity to stimulate naive and memory T cells (Banchereau et al., 2000, Nauta et al., 2006, Wang et al., 2013). The aim of the present study was to assess the effect of MSCs on DC maturation. Materials and Methods. MSCs were collected from femurs of male Wistar rats. Cells suspension were cultured in DMEM/F12 supplimented with 10% fetal calf serum (FCS), 5% horse serum and 1% antibiotic– antimycotic (Gibco). DCs were prepared from rat bone marrow after red cells lysis and cultured in RPMI 1640 medium (Gibco) supplemented with 10% FCS, 1% antibiotic–antimycotic (Gibco), 10 ng/mL GM-CSF (Sigma), and 5 ng/mL IL-4 (Sigma). MSCs and DCs were cultured for 7d at 37°C. DCs (5x105) were grown in two different conditions: co-culturing with MSCs and 25 ng/ml TNF (I) or without MSCs and 25 ng/ml TNF (Sigma) (II) for 48d. Cell phenotype were characterized by flow cytometry (FACSCanto II) using CD11b, CD44, CD86 (Becton Dickinson) antibodies. Results and Conclusion. After co-culture with MSCs, DC showed a decrease in CD86 expression compared with culture supplemented only with TNF which showed an increase in expression of this marker. Acknowledgements-This work was supported by Forerunner Federation

BIODIVERSITY OF THE CORONARY ARTERIES IN CATTLE - MACROSCOPIC STUDY

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 2
Written by Iulian DUMITRESCU, Gabriel PREDOI, Cristian BELU, Bogdan GEORGESCU, Petronela ROȘU, Florina DUMITRESCU

Lately, experimental medicine used the ruminants as experimental animals. On the sheep were achieved even heart experimental surgery. However, the literature about the vascularization of the heart is not very numerous especially regarding large ruminants. This study was conducted to provide supplementary data for the literature. The study was carried out on a total of 12 specimens in which the hearts were dissected after insertion into the arteries of the contrast dye. It has been found that right coronary artery is smaller than the left. Subsinusal branch is given by left coronary artery, being an extension of the left circumflex branch in subsinusal groove. Right ventricular wall is crossed by 5-6 main collaterals of right coronary artery, some of their terminal branches showing anastomosis with corresponding branches of the paraconal branch of the left coronary artery. Right coronary artery is much better represented than the left. Its terminals have a size nearly equal.

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DUMITRESCU I., PREDOI G., BELU C., GEORGESCU B., ROȘU P., DUMITRESCU F. 2016, BIODIVERSITY OF THE CORONARY ARTERIES IN CATTLE - MACROSCOPIC STUDY. Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 2, ISSN 2065-1295, 27-33.

BIOLOGICAL OSTEOSYNTHESIS: MINIMINAL INVAZIVE PLATE OSTHEOSYNTHESIS VS. CASTING/SPLINTING

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Adelina PROTEASA, Roxana DASCĂLU, Larisa SCHUSZLER, Cornel IGNA

In Romania, in the orthopedic veterinary practice, splinting/casting was considered an acceptable treatment of some types of fractures, namely, stable, closed reductible fractures.The greatest advantage of this type of asset is the biological osteosynthesis, favored by indirect reduction, preservation of the blood supply of all fragments, including small ones, which easily can turn into bone sequesters when regional vasculature is impaired. External fixation provided by casts and splints has several significant advantages compared with internal fixation methods: no need for implants, low postoperative infection rate, minimal disruptions of the fracture hematoma and the low cost of the procedure. Complications that can occur, usually due to improper selection of cases, inaccurate application technique and / or poor postoperative management can be minimized by using the minimally invasive plate osteosynthesis, maintaining the pros of bone healing.

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Proteasa A., Dascălu R., Schuszler L., Igna C. 2015, BIOLOGICAL OSTEOSYNTHESIS: MINIMINAL INVAZIVE PLATE OSTHEOSYNTHESIS VS. CASTING/SPLINTING. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 83-87.

BIOSECURITY OF TRADITIONAL PRODUCTS OBTAINED FROM SHEEP AND COW GRAZING IN ALPINE CONDITIONS OF THE COM. DOFTANEI VALLEY, JUD. PRAHOVA

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Ioana COJOCARU, Andrei TANASE, Dorin TOGOE, Alexandru VITALARU

Alpine Grazing is a common practice used in sheep and cattle breeding. Milk from this number is processed and converted into local traditional products. Biosecurity products is ensured mainly by the short time between milking and processing , which prevents the multiplication of germs of any kind. Laboratory determinations ; TPC(total plate count) made for control and detection of pathogens (E. coli ) show that TPC(total plate count) is lower than for analyzes of milk from farms organized and collected , harvested at the factory for processing

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Cojocaru I., Tănase A., Togoe D., Vitalaru A. 2015, BIOSECURITY OF TRADITIONAL PRODUCTS OBTAINED FROM SHEEP AND COW GRAZING IN ALPINE CONDITIONS OF THE COM. DOFTANEI VALLEY, JUD. PRAHOVA. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 211-213.

BLOOD MINERAL STATUS INFLUENCE ON MINERAL NUTRITIONAL VALUE OF MILK OBTAINED FROM A DAIRY FARMING INTENSIVE SYSTEM

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 2
Written by Gheorghe V. GORAN, Elena ROTARU, Liliana TUDOREANU, Emanuela BADEA, Victor CRIVINEANU

Milk from cattle species is an important part of human alimentation due to its mineral content, among other nutritional substances. Researchers have conducted studies to improve milk nutritional value, even reaching genetic manipulation in order to enrich cow milk with lysozyme, lactoferrin, and lactalbumin, components usually found in human milk. Mineral concentration in milk is an important quality parameter for human nutrition. Mineral concentrations from raw milk and blood were measured by inductively coupled plasma optical emission spectrometry (ICP-OES) in samples taken from 15 dairy cows farmed in a dairy farming intensive system situated in the south of Romania. Blood concentrations of all analyzed minerals registered strong difference to their concentration in milk. However, strontium concentration in milk is linearly correlated to calcium concentration in milk. This finding suggests that calcium and strontium use similar transporters at cellular level and compete for the same transport system. There has not been found a positive correlation between the milk obtained from daily production and the blood level of some minerals, although it can be observed that at productions of 53.9 L/day all elements, with the exception of iron, have higher levels comparative with the lowest milk production (15 L milk), that could be explained by the differentiated feeding required by the productive capacity of animals.

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GORAN Gh.V., ROTARU E., TUDOREANU L., BADEA E., CRIVINEANU V. 2016, BLOOD MINERAL STATUS INFLUENCE ON MINERAL NUTRITIONAL VALUE OF MILK OBTAINED FROM A DAIRY FARMING INTENSIVE SYSTEM. Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 2, ISSN 2065-1295, 94-99.


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