BONE RECONSTRUCTION METHOD BY CERCLAGE IN COMMINUTED FRACTURES OF LONG BONES DOG AND CAT

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Andrei TĂNASE

Reconstruction of a long bone marrow is recommended in literature to remedy job by fixation with plates and screws or external fixation. In cases where small shirks are interested and a significant portion of bone length two metals are difficult to apply and sometimes resorting to amputation of the affected limb. Fix these cases with multiple cerclage if the most times to recovery of the same dimensions of the member member congener . Formerly an experiment performed on 4 cases of dogs and cats 5 cases of comminuted fracture which suffered femur and care were recovered version excess of 85%.

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Tănase A. 2015, BONE RECONSTRUCTION METHOD BY CERCLAGE IN COMMINUTED FRACTURES OF LONG BONES DOG AND CAT. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 81-83.

BOTULINUM NEUROTOXIN SEROTYPES INVOLVED IN FOOD-BORNE BOTULISM OUTBREAKS IN ROMANIA IN THE LAST FIVE YEARS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Laurenţiu Mihai CIUPESCU, Isabela Madălina NICORESCU, Rodica DUMITRACHE, Rodica TANASUICA, Veronica CIUPESCU

This paper summarizes five food-borne botulism outbreaks occurred in Romania from January 2010 till the beginning of 2015. In this period, from 54 food samples received from human botulism suspicion cases and 140 self-control samples, only five samples were positive to the botulinum neurotoxin detection by mouse bioassay. Traditional prepared food seems to be the most common way to get the causative bacteria from specific poisoning areas. The food matrices positive for BoNT were pork and fish meat, all of them made at home in a traditional way. The most frequent BoNT serotype incriminated was B, found in three outbreaks associated with homemade salted and smoked dried pork and one outbreak with a homemade salted and smoked-dried fish meat. Only in one case, two BoNT serotypes A and B were detected in the same sample (salted and smoked- dried pork meat). For certain regions, seems to be incriminated a certain type of BoNT. Amongst the five outbreaks, three were reported in the North-Western, one in the Western and one in the Southern area of the country, thereby these places could be assigned like botulism poisoning zones, but studies need to be continued.

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Ciupescu L. M., Nicorescu I. M., Dumitrache R., Tanasuica R., Ciupescu V. 2015, BOTULINUM NEUROTOXIN SEROTYPES INVOLVED IN FOOD-BORNE BOTULISM OUTBREAKS IN ROMANIA IN THE LAST FIVE YEARS. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 238-243.

BRACHYCEPHALIC AIRWAY SYNDROME IN DOGS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Andreea- Bianca BOFAN, Iuliana IONAȘCU, Alexandru ȘONEA

Brachycephalic syndrome in dogs, also called congenital obstructive disease of the upper airway is described as a continuous process of anatomical and functional disorders of the respiratory and digestive systems. The syndrome is characterized by stenotic nares, elongated soft palate, everted laryngeal saccules and in advanced stages by laryngeal collapse. Clinically, dogs show signs of respiratory distress, such as: severe dyspnea, wheezing, coughing, snoring, exercise intolerance, increased respiratory effort, hyperthermia and collapse, and digestive signs, as: vomiting, regurgitation, ptyalism, pyloric stenosis and inflammation of the gastrointestinal segments. Medical management includes weight control and reduced physical effort. Patients with acute respiratory syndrome should be treated as an emergency. Surgical treatment requires wedge resection of stenotic nares or rhinoplasty, shortening of the soft palate or palatoplasty, and removal of laryngeal saccules. Early recognition and correction of brachycephalic airway syndrome has favorable long-term outcomes for the patient.

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Bofan A. B., Ionașcu I., Șonea A. 2015, BRACHYCEPHALIC AIRWAY SYNDROME IN DOGS. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 103-112.

CAMPYLOBACTER’S PREVALENCE IN ROMANIA – A COMPARISON WITH THE PREVALENCE IN EUROPE

Microbial food safety is an increasing public health concern worldwide. Campylobacter is a bacterium that can cause an illness called campylobacteriosis in humans. With over 200000 human cases annually, this disease is the most frequently reported food-borne illness in the European Union (EU). Campylobacter bacteria are a major cause of foodborne diarrheal illness in humans and are the most common bacteria that cause gastroenteritis worldwide. Campylobacteriosis are largely perceived to be food-borne, with poultry meat as a major source. The interest in determinations of Campylobacter in Romania started very recently, the first monitoring recording in 2007. Since then, every year there are tested about 450 samples represented either carcass skin from slaughterhouse or retail raw chicken meat. All samples have been performed accordingly ISO 10272/2006. The highest incidence of Campylobacter (63%) was observed in 2008, whereas in next years it was remarked a decrease. This decrease appeared especially consequently of implementation of hazard analysis of critical control point (HACCP). In the same period the Campylobacter’s prevalence in Europe was different, depending on country. The most frequently strains isolated were Campylobacter jejuni and Campylobacter coli, these being the main strains involved in human campylobacteriosis pathology.

CEREBRAL EDEMA/OEDEMA

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Cristina FERNOAGA, Mario CODREANU, Mihai CORNILA

Intracranial pathology in dogs is often associated with brain edema. Following acute injury, brain edema reaches it’s maximal peak between 24 to 48 hours and may persist for a week or more. Brain edema has been categorized as vasogenic, cytotoxic, or interstitial based on cause and anatomic areas of involvement. Any or all of these types of edema may be present in an animal with brain disease. By definition cerebral edema is the excess accumulation of fluid in the intra-and/or extracellular spaces of the brain. Cytotoxic edema occurs as a consequence of / pump disfunction, resulting in neuronal distress. The volume of intracellular fluid increases and cells begin to swell. This edema oftenly occurs due to disease processes such as toxicity, ischemia, or hypoxia. Interstitial edema is defined as increased fluid content in the periventricular white matter as a result of CSF movement across the ventricular walls in instances of hydrocephalus. Periventricular white matter is reduced as a consequence of the myelin lipids dissapearance secondary to an increase in hydrostatic pressure or decrease in periventricular blood flow of the white matter . Vasogenic edema is the most common form of edema associated with CNS neoplasia. These abnormalities allow fluid to move from the vascular to the perivascular spaces. Deep white matter of the involved cerebral hemisphere is preferentially affected. The “ideal” drug in treating cerebral edema, focused on mobilizing and/or preventing fluid accumulation in the brain, with a rapid onset, prolonged action and minimal side effects, is yet to be discovered.

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Cristina FERNOAGA, Mario CODREANU, Mihai CORNILA 2013, CEREBRAL EDEMA/OEDEMA. Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4, PRINT ISSN 2065-1304, 55-58.

CHARACTERIZATION OF THE MAIN VISCERAL LESIONS IDENTIFIED IN PSITTACINES DEAD FROM DIFFERENT CAUSES

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Iulia-Alexandra PARASCHIV, Raluca Ioana RIZAC, Andrei Constantin STOIAN, Emilia CIOBOTARU, Laurentiu TUDOR, Manuella MILITARU

Exotic bird pathology is an emerging field, needed for an accurate understanding of the biology and disease response of these species. The present paper is aimed to emphasize different lesions in some organs of psittacine cases submitted to necropsy due to sudden death, tumoral disease or metabolic disease. The study was conducted over a two year period (2013-2014) at the Department of Pathological Anatomy from the Faculty of Veterinary Medicine,.Bucharest. Seven psittacine cases, from three different species were submitted to diagnosis. The following organs were submitted to gross and histopathologic examination for each case: lung, heart, liver, kidney, gastro-intestinal tract, spleen and brain. Lesional changes in the organs were classified as: inflammatory, circulatory, necrotic, distrophic and tumoral. The lung presented circulatory lesions in all seven cases and for one case tumoral lesion as well, while the kidney presented both circulatory and necrotic changes in five of the seven cases. The heart was affected in two cases of necrosis, one case of distrophy and one case of tumoral lesion. The liver was affected in two cases by circulatory lesions and one case of inflammatory lesion. The brain was affected in two cases by inflammatory lesions and one case of circulatory injury. The gastro-intestinal tract was affected in one case of necrosis and the spleen, in one case of circulatory lesion. In addition, normal aspects were observed in nine organs, mostly in heart, liver and, spleen. Post-mortem transformation was noticed in 21 organs, mostly kidneys, gastro-intestinal tract, spleen and brain. In conclusion, circulatory and necrotic lesions were frequently encountered in the studied cases. Regarding nonlesional changes in the organs, these appeared with increased frequency, proving the importance.of a rapid diagnosis.

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Paraschiv I. A., Rizac R. I., Stoian A. C., Ciobotaru E., Tudor L., Militaru M. 2015, CHARACTERIZATION OF THE MAIN VISCERAL LESIONS IDENTIFIED IN PSITTACINES DEAD FROM DIFFERENT CAUSES. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 147-152.

CLINICAL AND HISTOPATHOLOGICAL MONITORING OF DEXTRAN SULFATE SODIUM COLITIS IN CD1 MICE

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Sonya CIULEAN, Orhan RASID, Leontina BANICA, Adina Daniela IANCU, Manuella MILITARU, Crina STAVAR

Mouse models of inflammatory bowel disease (IBD) are important tools in the study of this yet misunderstood pathology. The dextran sodium sulfate (DSS) colitis model has several advantages, however, like any animal model, thorough experimental set-up is required. We aimed to set-up a DSS colitis model and to establish means of monitoring disease progression, with or without the influence of oral vitamin D3 supplementation. Colitis was induced by administration of DSS in drinking water to adult Balb/c and CD1 mice. A vitamin D3 supplement was given to CD1 mice by oral gavage as therapeutic attempt. Clinical signs and body weight were monitored daily. Histopathological analysis of colon sections was performed using hematoxylin-eosin staining. Compared to inbred Balb/c mice, which succumbed to 2,5% DSS treatment without specific signs of colitis, outbred CD1 mice developed the full spectrum of clinical colitis at a regimen of 5% DSS. Histopathology of colon sections showed different degree of leukocyte infiltration and mucosal alteration. Characteristic manifestations of disease were reversible by both long and short oral vitamin D3 treatment. Our preliminary results show that the CD1 mouse strain is more suitable than the Balb/c strain for studying colitis in a DSS induced model. The established model may be a useful tool to elucidate the mechanisms involved in IBD pathogenesis and for therapeutic efficacy.

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Sonya CIULEAN, Orhan RASID, Leontina BANICA, Adina Daniela IANCU, Manuella MILITARU, Crina STAVAR 2013, CLINICAL AND HISTOPATHOLOGICAL MONITORING OF DEXTRAN SULFATE SODIUM COLITIS IN CD1 MICE. Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4, PRINT ISSN 2065-1295, 9-14.

CLINICAL DIAGNOSIS IN CANINE DEMODICOSIS. A NEW APPROACH

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Andreea GARTNER, Gheorghe DĂRĂBUȘ, Corina BADEA, Florin HORA, Elena TILIBASA, Narcisa MEDERLE

Canine demodicosis is caused by Demodex canis mite found in hair follicles. Demodicosis is a nonpruritic dermatosis which frequently becomes pustular by bacterial complications. The evolution of demodicosis as clinical disease takes different aspects, from dry to festering, from a manifestation of generalized or localized to one particular. According to current research, symptoms of demodicosis are constantly changing influenced by various favourable factors, an aspect that creates confusion in clinical approach and thus prevent correct diagnosis. In this context, the aim of the study was to bring current information on clinical diagnosis in canine demodicosis. The study was performed from September 2011 to December 2014, on a total of 187 dogs diagnosed with demodicosis microscopically. Clinical signs followed in this study were: erythema (”demodectic spots”), hair loss (”demodectic glasses”), follicular keratosis, hyperpigmentation, hyperseborrhea, pruritus. We also followed the evolution of the disease forms: dry demodecosis with nummular forms (circinate) and diffuse alopecia, piodemodicosis, pododemodicosis and otodemodicosis. The results revealed the absence of typical lesions: ”demodectic glasses”, ”demodectic spots”, occurrance of hyperpigmentation and itching in dogs with dry demodicosis (untypical for this form of clinical evolution and appearance of itching, generalized erythema and alopecia as a single clinical signs evolving. Specific localizations (pododemodicosis and otodemodicosis) were diagnosed without combination with other pathogens and clinical manifestations common to several pathogenic entities (itching, ihor smell, collections ear like ”coffee grounds”, blistering interdigital). The results contribute to the complex diagnosis of one of the most common and important diseases of parasitic nature of the dog.

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Gartner A., Dărăbuș G., Badea C., Hora F., Tilibasa E., Mederle N. 2015, CLINICAL DIAGNOSIS IN CANINE DEMODICOSIS. A NEW APPROACH. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 76-80.

CLINICAL FOLLOW-UP OF DOGS WITH NEUROLOGICAL DISORDERS AND POSITIVE FOR ANTIBODIES AGAINST TOXOPLASMA GONDII

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Cristina FERNOAGĂ, Mario D. CODREANU, Mihai CORNILĂ, Raluca Teodora NAE, Mariana IONITA, Ioan Liviu MITREA

Toxoplasmosis is an important parasitic zoonosis caused by the protozoan Toxoplasma gondii, which is widespread in humans and animals worldwide, including dogs. Infection with T. gondii in dogs is usually asymptomatic but cases of severe clinical toxoplasmosis have been reported worldwide. Toxoplasmosis is recognized as an opportunistic disease in dogs and is characterized by neuromuscular, respiratory, and gastrointestinal signs or by generalized infection. The most common neurological signs are characterized by ataxia, circling, behavioural changes, seizures, paralysis, paraplegia, twitching and tremors. In this study we present a clinical follow-up of twenty-seven dogs admitted to the Veterinary Clinic of Faculty of Veterinary Medicine of Bucharest in 2014, presenting with neurological disorders, mainly epilepsy-like seizures. All of them were serological positive for antibodies against T. gondii, as follows: 63.00% (17/27) were positive for both IgG and IgM, 25.9% (7/27) were positive only for IgG, while 11.1% (3/27) were positive only for IgM. The clinical presentation and the outcome of different therapeutic regimes are discussed. The study demonstrates the importance of toxoplasmosis in dogs and represents a basis for further in-depth studies to investigate the risks for clinical canine toxoplasmosis and to confirm, including by PCR, the implication of T. gondii in the neurological pathology in dogs in Romania. In addition to its veterinary importance, toxoplasmosis is also of major zoonotic concern and dogs can serve as epidemiological indicators for local infections

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Fernoagă C., Codreanu M. D., Cornilă M., Nae R.T, Ionita M. , Mitrea I.L. 2015, CLINICAL FOLLOW-UP OF DOGS WITH NEUROLOGICAL DISORDERS AND POSITIVE FOR ANTIBODIES AGAINST TOXOPLASMA GONDII. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 131-134.

CLINICAL PREVALENCE OF METHICILLIN RESISTANCE STAPHYLOCOCCI IN A PIG FARM FROM ARAD COUNTY – PRELIMINARY STUDY

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by János DÉGI, Iulia BUCUR, Voichiţoiu Robert VILI, Viorel HERMAN

Colonization with Staphylococcus aureus resistant to methicillin (MRSA) has recently been identified in pigs and that the people working in these sectors and increasing concern about the role of pigs as reservoirs of MRSA infections in humans were reported more frequently in the literature. The research had as purpose to determine the frequency of staphylococcal strains isolated from pig herds, phenotypic characterization of isolates and identification of methicillinresistant strains and types of resistance. Samples were taken from clinically healthy pigs from a swine farm in Arad, from October to December 2013. Samples required bacteriological examinations were collected from a total of 87 clinically healthy pigs. After growth, staphylococcal isolates were identied according to their characteristics as outlined in Bergey’s Manual of Determinative Bacteriology and the Manual of Clinical Microbiology. From pig farm were isolated 28 strains of staphylococci, including 20 coagulase positive strains (CoP, represented by S. hyicus and S. aureus) and 8 coagulase negative strains (CON, represented by S. haemolyticus, S. epidermidis respectively S. sciuri), isolated from clinically healthy pigs in different anatomical areas. All strains of staphylococci isolated from pigs showed sensitivity of 100% for novobiocine, rifampicine, pristinamycin, ciprofloxacin, vancomycin, ceftriaxone, cefoxitin, cefaclor and ampicillin/sulbactan, considered the drug of choice for these bacteria. As against used β-lactams (methicillin, ceftriaxone, cefoxitin, cefaclor, ampicillin with sulbactan) antibiotic sensitivity was highest, except methicillins, which were isolated 4 resistant strains, two S. hycus methicillin-resistant strains and two S. aureus strains. After testing staphylococci strains isolated from pigs, against 17 antibiotics were identified methicillin-resistant strains and several types of resistance to β-lactams, tetracyclines, macrolides and polymyxin B.

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Dégi J., Bucur I., Vili V. R., Herman V. 2015, CLINICAL PREVALENCE OF METHICILLIN RESISTANCE STAPHYLOCOCCI IN A PIG FARM FROM ARAD COUNTY – PRELIMINARY STUDY. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 157-162.


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