PRINT ISSN 1222-5304, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Laura PARLAPAN, Simona CIUPE, EmÖke PALL, Mihai CENARIU, Iulia Maria BALACI, Sandu Nicolae PÎNDARU, Ioan Stefan GROZA

During the last decades a technique based on membrane permeability to fluorescent dyes has been developed in order to determine these parameters. The aim of this study was to determine the fresh boar sperm viability by flow cytometry using a triple stain protocol. Fresh boar semen samples were analyzed with FACSCanto II (BD Biosciences) system on the basis of Hoechst 33348, peanut agglutinin-fluorescein isothiocyanate (FITC-PNA) and propidium iodide (PI) staining. Flow cytometric provide an accurate and precise technique which allowed detection of four categories of sperm: live sperm with intact acrosomal and plasma membranes, live sperm with acrosome lesions and intact plasma membrane, dying sperm with damaged plasma membrane and intact acrosome, as well dead cells with damaged plasma membrane and acrosome lesions.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Cornel IGNA, Roxana DASCALU, Larisa SCHUSZLER, Daniel BUMB, Adelina PROTEASA

This study used radiographic techniques to evaluate the Insall Salvati index (ratio of the length of the patellar ligament to the length of the patella - L:P) to compare the verticality patellar position at small-medium and large breed dogs that were grouped in healthy dogs, dogs with patellar luxation and with cranial cruciate ligament ruptures. Except for the based on L:P ratio measurement at small-medium breed dogs and in large-breed there was no difference among the groups. In large large-breed dogs the LPL is associated with patella baja. No statistically significant differences of L:P ratio between dogs affected or unaffected with cranial cruciate ligament rupture are reported Further studies using other imaging techniques are required in small and large-breed dogs with patella luxation (LPL and MPL).

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