PRINT ISSN 1222-5304, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

DEMONSTRATION OF CLUMPING FACTOR USING A SCREENING TEST IN STAPHYLOCOCCI ISOLATED FROM ANIMALS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Iulia BUCUR, Oana PETREC, D. MITRICĂ MĂRĂCINE, J. DEGI, L. FLUERAȘU

The clumping factor is a chemical compound found in the bacterial cell wall, which reacts directly with the fibrinogen, without using a plasma factor, causing the aggregation of bacterial cells in clusters with fibrin masses. The demonstration of clumping factor can be done with rapid kits containing latex particles sensitized with fibrinogen and IgG. Strains that have clumping factor or protein A, brought into contact with the kits ingredients, on a glass slide, produce an agglutination of the mixture. Research was made in order to detect the clumping factor in the staphylococci strains isolated from animals and, based on biochemical properties, were included in the following species: S. aureus ssp. aureus (82 strains), S. intermedius (114 strains), S. hycus (11 strains) and S. xylosus (12 strains). For the staphylococci strains, the clumping factor’s presence was tested with a commercial kit and the obtained results are the following: - S. aureus ssp. aureus (64 strains possessed clumping factor); - S. intermedius (79 strains possessed clumping factor); - S. hycus (the tested strains didn’t possess clumping factor); - S. xylosus (the tested strains didn’t possess clumping factor). The results show that the production of clumping factor is correlated with the species and also with the pathogenicity of the staphylococci strains.

[Read full article] [Citation]

The publisher is not responsible for the opinions published in the Volume. They represent the authors’ point of view.

© 2019 Scientific Papers. C Series. Veterinary Medicine. All Rights Reserved. To be cited: Scientific Papers. C Series. Veterinary Medicine.

Powered by INTELIDEV