PRINT ISSN 1222-5304, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Poliana TUDOR, Romanita MATEESCU

With this case report, we describe Dermacentor reticulatus ticks infestation in a Common Starling (Sturnus vulgaris) which was brought to our clinic by a citizen who involuntarily hit by car, in June 2012. The bird had massive infestation with ticks in the head and neck. Twenty-nine ticks were collected, and on the basis of the morphological criteria have been identified as Dermacentor reticulatus.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIII, Issue 2
Written by Constantin CHIURCIU, Mădălina TABLICĂ, Lucica SIMA, Teodora SUPEANU, Mariana OPORANU

The present research focuses on the determination of the immunoglobulin Y (IgY) levels derived from egg yolks obtained from hens previously inoculated with a combination of bacterial and fungal antigens (multiple antigens). The purpose of the work consists of establishing the frequency of the inoculations, in order to obtain a high level of antibodies throughout the duration of the experiment. The antigens were obtained from inactivated bacterial and fungal humans pathogenic strains. Egg-laying hen lots were formed out of individuals at the beginning of the egg-laying period. The hens were inoculated with multiple antigens, three times, at the first, 14th and the 28th day of the experiment. The control for the immune response was performed by sampling eggs on the 14th day from the third inoculation process. The IgY was extracted from the egg yolk in order to obtain the aqueous phase. The characterization of the IgY titers was performed every 30 days, for a period of 9 months, by using qualitative and quantitative ELISA assays. Following the end of the 9 months period since the 3rd inoculation process, the specific IgY titers maintained at high levels, another (4th) inoculation shouldn’t be necessary.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIII, Issue 2
Written by Bianca MATOSZ, Cristian DEZDROBITU, Cristian MARTONOS, Vlad LUCA, Aurel DAMIAN

Salivary glands are important in research articles, because of their different functions (Asari et al., 2000). They develop in different locations, having a very various architecture, secreting different types of saliva (Jaskoll et al., 2002). The major salivary glands are parotid, mandibular and sublingual glands. These glands have an important role in aliments’ digestion through their secretions (saliva), which is serous, mucous or sero-mucous, containing different enzymes, water, mucopolysaccharides and lubrefiant glycoproteins (Al-Saffar and Simawy, 2014; Boșca et al., 2014). In this study, we used five healthy male rabbits from a private breeder in Cluj, sacrificed by the owner for own consumption. Subsequently, the anatomical regions of major salivary glands were shaved and cleaned with alcohol. Macroscopical exam revealed that the rabbits’ parotid gland is the most voluminous gland, having the aspect of a scythe blade. The mandibular gland has a lobate appearance. We noticed that there is an asymmetry between the two mandibular glands, the right one being more developed than the left one. Sublingual gland is the smallest gland, being covered by surrounding connective tissue. Inferior buccal glands gathered and formed a major salivary gland, the labial gland, situated at the lips commissure.

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The purpose of this article is to review the most modern techniques used in obtaining transgenic pigs as important models in the study and understand of mechanisms involved in the pathogenesis of various human diseases. Genetically engineered animals have a significant role in biomedical research (Wheeler 2007, Robl et al., 2007, Vajta et al., 2012). Genetically modified swine are recognized as outstanding models for the study of various human diseases. These animals have high reproductive capacity and adequate precocity, short farrowing interval, parturition of multiple offspring (an average of 10–12 piglets per litter) (Wolf et al., 2000, Walters et al., 2012). Besides all these aspects the completed sequencing of the swine genome (Schook et al., 2005), made possible precise representations of human diseases based on propinquity of genes between the two species (Whyte, Prather 2011). Pigs are recognized as excellent models in a variety of areas, including nutrition, toxicology, dermatology, diabetes, cancer, atherosclerosis, cardiovascular disease, cutaneous pharmacology, ophthalmology, degenerative disease etc. (Lunney et al., 1999, Ishii et al., 2006, Herkenne et al., 2006, Dyson et al., 2006, Du et al., 2007, Matsunari, Nagashima 2009, Aigner et al., 2010, Noel et al., 2012, Prather et al., 2013). Due to the emergence of modern genetic engineering techniques, the genome of these animals can be modified to serve as a potential xenograft donor (Sachs, Galli 2009).

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIII, Issue 2
Written by Cornel IGNA, Larisa SCHUSZLER

Cranial cruciate ligament (CrCL) breaks in dogs can be treated by surgical and non-surgical methods. Choice of the treatment method of cranial cruciate ligament rupture in dog continues to constitute a real problem for veterinarian clinicians. This topic has been the subject of many studies. The investigation of the speciality literature data concerning the surgical treatment options in the management of cranial cruciate ligament breaks in dog, remains in the conditions of an informational avalanche a present concern. The purpose of this study was to analyze additional evidence which have appeared in the literature in the period of 2006 - January 2017 and which advocate with concrete evidences in the favour or disfavour of a particular method of dog’s cranial cruciate ligament breaks treatment. Analysis of online searches using PubMed engine in 403 articles suggest that the data analyzed do not allow accurate comparisons between different treatment procedures of cranial cruciate ligament deficiency in dogs and did not show significant differences and major changes compared to previous reports (from 1963 to 2005). New long-term clinical studies must designed and further biomechanical and kinematic analyses are required to determine the optimal technique, and whether these procedures are superior to other stabilization methods.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Bogdan GEORGESCU, Gabriel PREDOI, Manuella MILITARU, Aneta POP, Letitia PURDOIU

After nearly a decade of lack of the component organized in practice centers in preparing students from the Faculty of Veterinary Medicine Bucharest, since 2009, this activity was revived by visits in the practice centers of groups accompanied by teachers with norm of practice. The study was conducted with three groups of 25 students and was held in the stages of the practice in 2010, 2011 and 2013. This practice activity offered the students the opportunity of applying knowledge acquired during the academic year/years. The groups of students who took the practice stage at Tulcea Sanitary Veterinary Department had the opportunity to perform various activities (baby boar castration, artificial insemination in sow etc.) on farms in the county. Students also actively participated in the maneuvers and activities of various departments of County Sanitary Veterinary Laboratory subordinated to SVD (necropsy, trichinoscopy, etc.). After completion of the program most students expressed their desire to visit different sights of Dobrogea. Thus, they had the opportunity to visit museums subordinated to Tulcea Eco-Museum Research Institute (Ecotourist Museum Center "Danube Delta" - Aquarium, Museum of History and Archaeology, Ethnography and Folk Art Museum), all of which are in the municipality area. On weekends, students were in boat rides on the Danube Delta canals, made equestrian tourism in Macin Mountains National Park, visited various archaeological sites and ancient and/ or medieval citadels (Aegyssus/ Tulcea, Noviodunum/ Isaccea, Enisala/ Heracleea, Histria, Argamum). The beautiful touristic journey included visits at Dobrogea Gorges and Gura Portitei. This approach to practical activity including the cultural side, respectively the touring side, had a very positive feedback from students who have completed the practice stage in an efficient and pleasant manner by an optimal combination of these sides with education.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Cristina FERNOAGĂ, Mario D. CODREANU, Mihai CORNILĂ, Raluca Teodora NAE, Radu CONSTANTINESCU

Diabetes mellitus in cats and dogs is a complicated illnes and its monitorisation is a chalLenge for the clinician. Thus, fructosamine indicates high level of blood glucose. The increased value of serum fructosamine is found in patients diagnosed with diabetes mellitus and it reflects the degree of glycaemic control, being useful for an objective and proper monitorisation. This parameter is much more accurate than the value of serum glucose level, especially when dealing with cats, due to the fact that in this type of patients the level of blood glucose can be affected by induced acute stress. This study includes 36 diabetic patients,19 dogs and 17 cats, from the Department of Internal Medicine of the Faculty of Veterinary Medicine Bucharest, in the past year, for which frutosamine has been determined. This has been conducted in order to asses quick changes in therapy and to improve glycaemic control.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1
Written by Oana - Margarita GHIMPETEANU, Magda GONCIAROV

European Union states encourage preparing national guides to good hygiene practice and application of HACCP principles. It is encouraged the dissemination and use of national and Community guidelines. However, food business operators may use these guides on voluntary basis. Guides to good health practice must include adequate information on the risks involved in primary production and related activities and measures to combat such risks, including the relevant set of national and Community law or national and community programs. Using of the guides to good practice may help food establishments to control hazards and demonstrate compliance. The best practice guidelines are usually a combination of good hygiene practices (GHP) and HACCP- based elements and include: practice guidelines for the implementation of mandatory requirements; requirements for a raw materials; hazard analysis; pre-establishment of critical control points in the preparation and processing of food; preventive hygiene measures for handling sensitive and perishable products and prepared food for groups of consumers with increased susceptibility to illness, the need for documentation and records, protocols for data validation of shelf life of the product. Guides of good sanitary practice represent a simple, but effective mean to overcome difficulties that may arise in certain food establishments to implement HACCP procedures. National and community guides must provide guidance on good practice to combat health risks in primary production and related activities. General guide may suggest common hazards and controls of certain food activities and help the manager or the HACCP team in making food safety procedures or methods and appropriate record keeping.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIV, Issue 1
Written by Abdullah ÖZBİLGİN, Behiç COŞKUN

In this review, general characteristics of sainfoin and researches on the usability in animal feeding. Sainfoin is a perennial leguminous plant. It is a perennial feed crop with a total area of 1,914,391 hectares in Turkey. Sainfoin grows in pH 7-8 on calcareous soils on the northern slopes of valleys, plains and mountains up to 6 thousand meters in height. The content of nitrogen in the branches and leaves of the sainfoin plant is higher than in the beginning of flowering. The sainfoin contains condensed tannin. The condensed tannin removes protein degradation. Grazing ruminants may encounter many metabolic diseases related to feeding. The minimum plant condensed tannin concentration for this effect should be greater than 5 g / kg (CT) in dry matter. In the sheep study, parasitic infestation of the abomasum and small intestine causes large protein losses in sheep. İn a study reported that the presence of tannin in sheep fed had no effect on milk yield but increased the yield of tannin to 5.9%.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIV, Issue 1
Written by Vlad VUTA, Florica BARBUCEANU, Gabriel PREDOI, Constantin VLAGIOIU

Rabies is a fatal viral zoonosis of the central nervous system of mammals. Until recently, rabies was predominantly in domestic dogs, although outbreaks were reports in wildlife. The implementation of dog mass vaccination resulted in the disappearance of dog-mediated rabies in Europe and North America, but the disease unexpectedly re-emerged in wildlife. Oral rabies vaccination (ORV) programs in wildlife are highly effective in control and eradication the disease. After years of successful vaccination campaigns, many previously infected countries in Western, Central and Northern European have become free of rabies. All rabies vaccines used for wildlife immunization are derivatives of the original SAD strain. Currently, five vaccine strains are authorized in Europe and all are derivatives of the original SAD strain: Sad Bern, SAD B19, SAG2, GASGAS and V-RG vaccine (Vaccinia Recombinant Glycoprotein). In our paper, we described and analyzed the main characteristics of available vaccines from Europe market for oral vaccination of wild animals, taking into account the performance and the quality features. In Western Europe, rabies has been eliminated using oral vaccination with all available vaccines. Nevertheless, worries still exist related to the residual pathogenicity of attenuated live vaccines that could induce rabies in certain conditions.

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