Scientific Works Series C. Veterinary Medicine

PRINT ISSN 1222-5304, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 1
Written by Antonello BUFALARI, Alberto CROVACE, Antonio DI MEO, Vasilica-Flory PETRESCU Alexandra PETEOACA, Luisa PASCUCCI, Gabriele SCATTINI, Beatrice DEL SAL, Giulia MORETTI

Mesenchymal stromal cells are a population of adult stem cells with a vast potential for tissue engineering as well as regenerative medicine. Ununited Anconeal Process (UAP) and Legg-Calvé-Perthes (LCP) disease are two common growth pathologies in young dogs. Even if these diseases present different manifestations and etiopathogenesis, the attention of modern research has focused on the “restoration” of the same cells that were not developed correctly in these bone defects. The administration of Bone Marrow Mesenchymal Stromal Cells and Bone Marrow Mononuclear Cells containing MSCs in these two types of pathologies could be considered as an innovative but also conservative therapy, allowing the formation of new bone tissue in a minimally invasive way. In this preliminary study we evaluated the improvement of the clinical and also the radiographic condition in 2 dogs with UAP and in 3 dogs with LCP, treated with a single administration of autologous Bone Marrow Mesenchymal Stromal Cells and Bone Marrow Mononuclear Cells.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 1
Written by Elena CÂRCIUMARU, Emilia CIOBOTARU-PÎRVU

The study aimed to present the clinical and morphological findings in neglected dogs during the period of 2015-2018 recorded in a private veterinary practice, in Bucharest, Romania. The purpose of this study was to identify and classify forms of passive abuse along with their effects on canine individuals. During the aforementioned period, 41 dogs were included in the study. Routine clinical and paraclinical methods of examinations were used, as well as after treatment follow-up. Several types of passive abuse have been recognised, such as lack of water and food, shelter, veterinary care and absence of proper surveillance. Lack of veterinary attendance was identified in 65% of studied animals (n=27), 26% were deprived of shelter (n=11), and 20 % were deprived of food and water (n=8). The dogs presented specific clinical signs and morphological features of malnutrition (n=8, 20 %), extremely large tumoral lesions (n=17, 41%), or severe parasitic dermatitis (n=10, 24%). Eleven dogs (26%) were deprived of shelter and surveillance. Multiple types of neglect were identified in the same dog (n=20, 49%). Conclusions: 44% of animals were older than 8 years (n=18), twelve animals died (30%) and 29 individuals (70%) were fully or partially recovered cases.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVI, Issue 2
Written by Ivan PAVLOVIC, Snezana IVANOVIC, Milan P. PETROVIC, Violeta CARO-PETROVIC, Dragana RUŽIĆ-MUSLIĆ, Narcisa MEDERLE

In pasture breed condition helminth infection are common especially during late spring and autumn months. Research of goats and sheep parasites was made systematically last 10 years in Serbia. Most of the research related to gastrontestinal and something less about lung helminth infection. The research was carried out on several locations in Serbia in the period and included goat and sheep herds in the area of carried out in north, northeast, eastern, southern and south-eastern part of Serbia and at Belgrade area. We examined fecal samples using the Berman method. Slaughtered or dead animals we examined by necropsy and adult parasites separated from the lung section. Determination of adult and larval stage of parasites was based on the morphological characteristics. During our examination most abundant species was Dictyocaulus filaria, followed by Protostrongylus rufescens, Cystocaulus nigrescens and Muellerius capillaris.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 1
Written by Andreea ISTRATE, Alexandra PETEOACA, Radu CONSTANTINESCU, Giovanni ANGELI, Andrei TANASE

Fragmented medial coronoid process is part of the triad of developmental lesions causing elbow dysplasia, amongst ununited anconeal process and osteochondrosis of the distomedial aspect of the humeral trochlea, being the most common clinical entity that generates elbow pain and osteoarthrosis in dogs. In this study we compared computed tomography (CT) and radiological findings in 25 dogs presented with forelimb non-traumatic lameness, that were screened for elbow dysplasia and had a CT diagnosis of fragmented medial coronoid process. The radiographs were evaluated according to International Elbow Working Group guidelines and compared with CT images. A fragmented medial coronoid process was diagnosed in 6 dogs using radiographs and was visible in all dogs in the CT examinations. Because fragments are often poorly visualized on radiographic images, due to the fact that the medial coronoid process may remain cartilaginous, the fragment may not be completely detached or may superimpose on the radius, radiographic diagnosis is made mostly on secondary osteoarthritic changes. Thus, computed tomography examinations of the elbow joint have a much higher sensitivity in diagnosing this developmental lesion.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 1
Written by Alexandru Gabriel NEAGU, Mihai SĂVESCU, Giovanni ANGELI, Niculae TUDOR, Constantin VLĂGIOIU

The objective of this study was to identify disorders at the level of the thoracic wall as a determining factor in modified respiratory function in dogs and cats. A number of 130 cases (79 dogs and 51 cats) with respiratory diseases and showed changes in the thoracic wall were examined. The cases were examined by clinical and paraclinical methods in the Faculty of Veterinary Medicine from Bucharest and Perugia clinics. At the thoracic wall bone tissue were recorded: 15 cases (13 dogs and 2 cats) with changes in the spine, 5 cases (3 dogs and 2 cats) with changes in the sternum and 12 cases (7 dogs and 5 cats) with changes at the ribs level. The soft tissues of the thoracic wall were affected by neoplastic processes in 26 cases (21 dogs and 5 cats) and post-traumatic injuries in 46 cases (24 dogs and 22 cats). Additionally, 26 cases (11 dogs and 15 cats) were identified with diaphragmatic disorders. The assessment found that post-traumatic injuries had the highest prevalence and the imaging examination allowed the localization of lesions.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVI, Issue 1
Written by Andreea ISTRATE, Alexandra PETEOACA, Radu CONSTANTINESCU, Andrei TANASE

Scottish Fold cats have an unique appearance due to their forward-folded ears, being considered a defining feature of the breed. It is considered that the folded ears are a result of an underlying cartilage disorder, a genetic abnormality that predisposes the affected cats for another genetical condition of the musculo-skeletal system, termed osteochondrodysplasia. Osteochondrodysplasia is a painful and progressive syndrome characteristic only to the Scottish Fold breed due to a mutation in the TRPV4 gene. This condition is characterised by skeletal deformities, such as shortened feet and splayed phalanges, affecting primarily the hindlimbs, short, thick and inflexible tails, shortened caudal vertebras and ankylosing polyarthropathy of the affected joints. This paper has the objective of characterising the main bone and joint problems observed in a 1 year-old female Scottish Fold cat which presented for lameness and postural abnormalities of the hindlimbs.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVII, Issue 1
Written by Roxana-Mariana IGNĂTESCU (ȚÎMPĂU), Ana-Maria GOANŢĂ, Andreea-Bianca BOFAN, Alexandra BRAICA, Natalia RĂDULEA, Lucian IONIŢĂ

This paper underlines the role of kidney biopsy in the diagnosis of various kidney diseases in dogs. Even though it is an invasive method, kidney biopsy is often required to establish a definitive diagnosis and an accurate prognosis. Its use should always be considered after weighing the indications and potential complications. Several techniques are used to obtain kidney samples, such as percutaneous renal biopsy (blind or palpation technique or using ultrasound guidance, keyhole technique, laparoscopic biopsy) and surgical biopsy. Once the method has been chosen, the renal sample should only be obtained from the renal cortex. This procedure requires a patient that is stable to undergo general anesthesia or deep sedation. Kidney biopsy samples may be evaluated using light microscopy and special stains, transmission electron microscopy and immunofluorescence to obtain a diagnosis of certainty and to guide treatment options.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVII, Issue 1
Written by Cristian BELU, Iulian DUMITRESCU, Bogdan GEORGESCU, Petronela Mihaela ROȘU, Anca ȘEICARU, Ștefania RAITA, Sorina Andreea MIHAI, Mădălina DOBRILĂ, Oresti MIHELIS, Gabriel PREDOI

The teeth are made of hard materials, they represent the most resistant organs to destruction, often being collected from archaeological sites. They play a major role in establishing the species origin of fossils or corpses. A single tooth sometimes provides clues as to where it came from, and provides information about the animal's age, diet, and behaviour. The study was performed on the skulls of pigs, canines and cats, then described the anatomical characteristics of each type of tooth, insisting on teeth that have similar anatomical aspects and can cause confusion in identification. The dentition of pigs is adapted to the omnivorous regime, the molars being of the bunodont type, compared to carnivores where all teeth are of the brachiodont type. The stricter carnivorous diet of cats, compared to that of canids, translates into the specialization of dentition to a more pronounced second type, the morphological effect being the reduction of tuberculous molars. This motivated us to carry out the work, hoping that the results could be useful for the professional activities of veterinarians, but also forensic doctors.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 1
Written by Gavrilă ZAGRAI, Cristian BELU, Cerasela VASILESCU, Diana LICSANDRU, Aurel DAMIAN, Ana Maria ZAGRAI (MAIEREAN), Livia PĂUN

In this paper, the assessment is to investigate the effect of some synthetic glucocorticoid hormones (used frequently in therapy) on leukocytes (WBC and leukocyte formula), in rats. It is mentioned that the hormones used in this study (Prednisone, Dexamethasone and Betamethasone) have a large utilization in therapy due to the anti-inflammatory effect, overlooking in many cases the immunosuppressive effect. The determinations regarding the number of leukocytes and the leukocyte formula have been made using the IDEEX Analyzer, at the beginning and at the end of the experiment. As a result of the analysis it was revealed that the WBC%, at the end of the experiment, was higher, for all the experimental groups, comparing with the control group. After the interpretation of the obtained results regarding to the values of the leukocyte formula in the case of the experimental groups, it was shown increases of the percentages of neutrophils and basophils, decreases of percentages of eosinophils, lymphocytes and monocytes, comparing to the values obtained for the control group.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVI, Issue 2
Written by Florina RAICU, Constantin VLAGIOIU, Niculae TUDOR

The purpose of this article is to contribute to the subsequent establishment by the European Commission of maximum admitted limits for heavy metals in game meat. Heavy metals residues (Lead, Cadmium) are present in game meat because of the environmental excessive pollution and because of the hunting methods. Game meat samples (deer and wild boar meat) from Harghita county were analysed by: atomic absorption spectrophotometry. The results were compared with other categories of meat for which the maximum admitted limits are specified in the Regulation EC no. 1881/2006. The results were below the maximum admitted limits for other types of meat for which there is a specific European legislation, as following: cadmium concentration was highly below the highest admitted limit for meat categories. However for some samples, lead concentrations were comparable to the maximum admitted limit. Consequently, repeated analyses were performed to validate the results. The high quantities of lead residues could be explained by the hunting methods, but also by the samples collection close to the wounds produced by firearms. The hunting methods and the place for sampling too close to the wound caused by firearms (including the bullets) are responsible for finding high concentrations of residual lead in the analysed samples.

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