Scientific Works Series C. Veterinary Medicine

PRINT ISSN 2065-1295, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 1
Written by Ruxandra TUDOR, Andra DEGAN, Alexandru Gabriel NEAGU, Luigi CARSTINOIU, Gabriel PREDOI

This case report presents the perianaesthetic management of a geriatric dog that underwent a hemilaminectomy surgery for T11-T12 medullar compression. A 10-year-old crossbreed male was presented at the Faculty of Veterinary Medicine in Bucharest for neurological investigation of acute onset of right posterior unilateral paresis. The magnetic resonance investigation revealed T11-T12 medullar compression, with surgery indication (hemilaminectomy). The patient was premedicated with Fentanyl 3 mcg/kg and Midazolam 0.2 mg/kg administered intravenously (IV). Induction was made with Propofol 4 mg/kg intravenously. Afterwards, the patient was intubated and maintained with Isoflurane along with the administration of a constant rate infusion (CRI) with Fentanyl (3 mcg/kg/h) and Ketamine (1 mg/kg/h) and an epidural anaesthesia with Lidocaine (2 mg/kg). Postoperatively, the patient received a multimodal analgesia management to help her not to exhibit any pain levels.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 2
Written by Ștefania Mariana RAITA, Mara GEORGESCU

As Prussian carp (Carassius gibelio) is one of the most consumed local fish in Romania, the present study is focused on challenge testing of Nigella sativa fortified Carassius gibelio whole fish muscle sample morphological quality. The challenge testing involved exposure to atypical temperature conditions, other than those anticipated in the food industry, including stress temperature trial (exposure to storage temperature variations) for fresh Carassius gibelio whole fish, supplemented with 0.6% v/w Nigella sativa seed oil (NSSO). Histological evaluation of muscle structure was performed at day 3 of storage, for all samples. Histological assessment of dorsal skeletal fish muscle revealed no significant differences between the NSSO enriched challenged sample groups and the control group, after three days of storage. This study shows promising results for the possible use of Nigella sativa seed oil, as a natural solution for extending shelf life and enhancing the quality of cold-stored fresh Prussian carp.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVI, Issue 1
Written by Viorel ORDEANU, Marius NECȘULESCU, Diana M. POPESCU, Lucia E. IONESCU

The new conception of the sciences of life, One health, logically combines, in a unitary whole, the knowledge and practices of human medicine, veterinary medicine, agronomy, environment that can influence the public health, in one way or another. The first selection and integration of these seemingly disparate areas was made by military medicine, based on the experience of war medical protection and counterterrorism. Epidemics and pandemics can spread quickly, through travel and international trade, uncontrolled migration, military or terrorist attacks. Most biological warfare agents kill or incapacitate humans but also animals or plants and then contaminate the environment for a variable time forming a reservoir of pathogens.Disease of the live - stock and cultivated plants can lead to food crisis and can be used to defeat a country or unfair economic competition, and their effect, regardless of whether they are real or false attacks, can be very important. As a result, is required not only a close civilian-military (CIMIC) collaboration but also between the different departmental components of the state: Ministry of Health, Ministry of Agriculture, Ministry of Defense, Ministry of Finance etc..

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVII, Issue 1
Written by Diana-Larisa ANCUȚA, Maria CRIVINEANU, Teodoru SOARE, Cristin COMAN

The biological interface between the host tissue and the medical device used may influence clinical outcomes. Expected effects include osteoinduction, osseointegration, increased vascularity, and mechanical stability. The aim of the study was to evaluate the process of bone regeneration and potential local effects as results of insert implants treated with innovative biomaterials in the intercondylar rat femur. Two groups of rats, Wistar, were created to which titanium implants were applied to the femoral bone. In one of the groups, the titanium implant coated with Poly (3- hydroxybutyric acid-3-hydroxyvaleric acid) polymer microspheres enriched with fibroblast growth factor 2, vascular endothelial growth factor and bone morphogenetic protein 4 (VGF2) was inserted and in the other group, the implant was untreated. After 6 weeks of clinical monitoring, rats were euthanized, and the implanted femur was harvested for histological analysis. In this study the data obtained showed that VGF2-treated implants contributed to faster bone regeneration, with favorable local effects, compared to untreated implants in which signs of persistent inflammation were.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVI, Issue 1
Written by Iuliana IONASCU, Adina ARGASEALA, Seralp UZUN, Gina GIRDAN

Feline herpesvirus is associated with complex ocular disorders: buphthalmia, descemetocele, deep corneal ulcers or penetrating corneal wound, in many cases the enucleation of the eyeball being the unique surgical option. During a period of 2 years (2016-2018), in 11 kittens with the age between 4 weeks and 6 months, intrascleral prosthesis was performed as an alternative to eyeball enucleation. Seven cases needed corneal reconstruction: we performed cornealconjunctival flap in 4 patients and applied Vetshield® collagen contact lens in the other 3 patients, followed by a third eyelid flap in all patients. Periodic ophthalmologic examinations revealed a postoperative evolution without major complications. The silicone prosthesis is well tolerated by the tissue. Patients are receiving longterm local treatment with hyaluronic acid gel. Intrascleral prosthesis is an aesthetic option for keeping the eyeball into the orbit.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVI, Issue 2
Written by Catalin MICSA, Dorin ȚOGOE, Gina GÎRDAN, Maria RoxanaTURCU, Cristina PREDA, Andrei TANASE

A 9-year-old spayed female mixed Pit Bull Terrier of 33.2 kg was referred with a complaint of stranguria, pollakiuria, hematuria, and abdominal pain of 5 weeks duration, not responding to treatment. The results of physical examination were unremarkable. A abdominal ultrasound revealed a large mass on the wall of the urinary bladder. The regional lymph nodes and the other abdominal organs present normal sonographical features. Thoracic radiography showed no evidence of metastatic disease. Blood samples have been taken for biochemistry work, CBC and urine samples. The dog underwent a cystoscopy, histopathological examination of the retrieved specimen reveal T2N0M0 TCC (muscle-invasive transitional cell carcinoma). Due to the mass location on the wall of the bladder, surgery was not an option. Considering the result of the investigation, chemotherapy treatment has been applied: intravesical every 2-week cycle consisted of alternating epirubicin and 5-fluorouracil and intravenous holoxan. The treatment was well tolerated with no occurrence of any side effects. The abdominal ultrasound was repeated every 1 month and showed no progression of the disease.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 1
Written by Rita GOLBAN

The scientific investigations revealed in this research present the study in dynamics of blood immunological indices characteristic to non-specific resistance at the neonatal calves in different age periods. In the study are presented the aspects of these immunological indices, regarding their correlation in determining the cellular and humoral immunity. Important data reveal the correlation of the concentration of lymphocytic, leukocyte indices, the phagocytic, lysozyme and bactericidal activity, which justifies the importance of nonspecific humoral resistance of the newborn organism, which denotes the assimilation of antibodies by colostrum during the neonatal period as a result of the installation of colostrum immunity. The initiation of these studies has demonstrated the importance of nonspecific or inborn immunity, constituting the first line of defense against pathogenic microbial agents at newborn animals.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVI, Issue 2
Written by Maria Rodica GURĂU, Hasan Majid HAMEED, Dragoș COBZARIU, Doina DANEȘ

Avian colibacillosis produced by Escherichia coli, a common infection facing poultry flocks, affects poultry industry by the broad spectrum of induced entities: systemic acute infection - colisepticemia, localised infection as omphalitis, cellulitis, enteritis, salpingitis/peritonitis, cronic disease - choligranulomatosis, the chronic respiratory disease and many more. The E. coli infection is causing large losses for the poultry industry, impairing the performances: decreased egg production, with drown of the carcasses at slaughterhouse and the increased mortality rate, are recorded in the affected flocks. E. coli strains inducing poultry diseases are a specific group of strains, called Avian Pathogen E. coli (APEC) strains: these strains randomly share more virulence-associated genes. 13 E. coli isolates from different Romanian poultry outbreaks/flocks have been investigated for the presence of Iuc D and Pap C genes. The Pap C gene is responsible for adhesin assembly and its frequency is associated with the cystitis strain like Santo E. proves in his article from 2006. Gene Iuc D of the plasmid encodes a membrane-bound enzyme synthesizing N6- hydroxylysine, the first product of the aerobactin biosynthesis pathway. The DNA extraction was made using QIAamp cador Pathogen Mini Kit (Qiagen). The amplification protocol was: a cycle of denaturation at 94°C for 30 s followed by 25 cycles of 94°C for 30 s, 58°C for 30 s and 68°C for 3 min, and a cycle of 72°C for 10 min. The Iuc D gene was present in 100% (13/13) isolates. Pap C gene was present in 23.07% (3/13) isolates. Considering these preliminary results, it can say that Iuc D and Pap C genes are independently expressing their virulence and further research should be performed to establish the pathogenicity of each of the two genes together with other pathogenic genes.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVI, Issue 2
Written by Cristina CĂȘARU, Anca BULGARU, Doina DANEȘ

Medicinal plants have been used since the earliest times to treat different disorders of humans and animals. The aim of this study was to identify the plant, mineral and animal remedies used in the treatment of cattle diseases traditionally raised, in the submontane area of the Curvature Carpathians. Relevant information has been collected from 237 interviewed subjects on the treatments used to control diarrhoea, mastitis, external mammary gland injuries, mammary papillomatosis, indigestion, acute meteorism and respiratory disorders, foot injuries/ infections, external and internal parasite infections. There have been identified 56 plants, 8 mineral substances and 6 substances of animal origin used in the treatment of the aforementioned disorders. According to the answers of the interviewees, the most frequently used plants belong to the Asteraceae family (32.35%), followed by Rosaceae (17.64%), Aliaceae, Betulacea and Fabaceae (8.82%), and the most frequently used species were Robinia pseudoacacia, Artemisia absinthium and Sempervivum tectorum.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 2
Written by Ștefania Mariana RAITA, Mara GEORGESCU

On the background of the growing need for natural alternatives to fresh fish preservatives, the present study is focused on the assessment of Nigella sativa seed oil effect over the freshness and morphological structure of Carassius gibelio (Prussian carp) muscle tissue, during typical cold storage. The monitoring involved storage of fish samples at regular temperature conditions, recommended for the food industry (0 ± 1°C, on ice). The Carassius gibelio fish samples were divided into three groups: control group (C), without NSSO; test group 1, fortified with 0.1% v/w NSSO (T1) and test group 2, fortified with 0.6% v/w NSSO (T2). Histological and freshness monitoring reveal that treatment groups T1-T2 keep normal morphological structure of fish muscle throughout the monitoring period, while showing slightly improved freshness indicators (pH and TVB-N), compared to control samples. This study strengthens the proposal for NSSO as a natural enhancer of fresh fish quality and shelf life.

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