Scientific Works Series C. Veterinary Medicine

PRINT ISSN 2065-1295, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 1
Written by Gabriel GAJAILA, Iuliana GAJAILA

Graphene is an allotrope of carbon consisting of a single tightly packed layer of sp2 carbon atoms arranged in a hexagonal lattice. Graphene has unique properties that could revolutionize different areas, including biology, biomedical science, environmental agriculture and biotechnology. Many studies are aimed mainly at antibacterial effect of graphene. Antibacterial properties of graphene are manifested by directly interacting with the cell membrane. In this study we aim to investigate the aspects of the role of the biochemical composition of the bacterial cell wall in the manifestation of the antibacterial activity of the monolayer graphene on metallic and non-metallic substrates. Antibacterial activity was tested on Gram-positive and Gram-negative bacteria via cell-viability test. The differences in structure and composition of the Gram-positive and Gram-negative cell wall induce different interactions with monolayer graphene. At the same time, the different substrate for the graphene film significantly influences its antibacterial properties. Our observations from this study provide new insights for future studies.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVI, Issue 2
Written by Maria Roxana TURCU, Ruxandra PAVEL, Andra DEGAN, Gina GÎRDAN, Catalin MICSA, Ovidiu ROȘU, Lucian IONIȚĂ

This study was performed in order to evaluate two anesthetic protocols used for ovariectomy in turtles belonging to Trachemys scripta elegans species, between June and July 2019 at the Faculty of Veterinary Medicine of Bucharest. Patients aged one year old were divided in two study groups depending on the anesthetic protocol used. Group A was premedicated with Midazolam (2 mg/kg), Dexmedetomidine (0.1 mg/kg) and Ketamine (5 mg/kg) administered intramuscularly (IM) while group B had Midazolam (2 mg/kg), Buprenorphine (0.2 mg/kg) and Ketamine (2 mg/kg) IM. Induction was made with Isoflurane 5% in the induction chamber followed by Ketamine (10 mg/kg) given intravenously, in the supravertebral sinus (IV). All patients were intubated with an 18G IV catheter and maintained with Isoflurane 3% and 100% Oxygen. Patients in group B required two boluses of Ketamine (10 mg/kg/bolus) compared with patients in group A that didn’t have any additional doses. Therefore the recovery was smoother and faster in patients from group A.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 1
Written by Alexandra PETEOACA, Andreea ISTRATE, Ana GOANTA, Gina GIRDAN, Alina STEFANESCU, Andrei TANASE

The aim of this case report is to present an accessible therapeutic approach that facilitates the healing of extensive degloving wounds. A 3 months old dog was presented to the emergency room for traumatic injuries with extensive tissue necrosis and infection of the front limbs. The animal was recumbent, tachypneic and exhibited pain. The temperature, blood pressure, heart rate were in the normal range. Further investigation revealed no osseous implication and a moderate to severe anaemia. The emergency treatment was focused on pain management, fluid therapy and primary care of the wounds. The wound management followed this succession: wet-to-dry dressings for the first two days, surgical debridement, honey dressings, hydrocolloid dressings alongside supportive therapy. This approach promoted the healing process, resulting in a full reepithelialization of the affected area, eliminating the need for skin grafts or reconstruction techniques. The honey and hydrocolloid dressings have to be properly used in the correct phase to offer a good healing environment for reepithelialization. We concluded that wound management should be a global approach that combines systemic and nutritional support with topical healing promoters.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVI, Issue 2
Written by Camelia ION, Ioan Liviu MITREA, Mariana IONITA

Canine babesiosis is a potential life-threatening tick-borne disease affecting dogs worldwide. Early diagnostic and specific therapy in due time are emergency issues, as the infection may progress rapidly to severe, acute, and complicated potentially fatal disease. Here we describe a clinical case of babesiosis in a 6-year-old male Bichon Frise dog which was referred to the Clinic of Faculty of Veterinary Medicine of Bucharest, in April 2019, with clinical signs (fever, 39.9°C, jaundice, haemoglobinurie, weakness) compatible for babesiosis. Blood samples were collected and subjected for hematological and biochemical investigations, and for parasitological testing. The blood smear analysis showed intraerytrocytic piroplasms compatible for large Babesia form. Subsequently of the clinical and paraclinical investigations the dog was diagnosed with severe babesiosis, characterized by severe anemia (PCV=15.8%; Hgb=5.8 g/dL), neutropenia, trombocitopenia, and acute renal injury; a guarded to poor prognosis was considered. Accordingly, the therapeutic protocol aimed firstly to stabilize the animal by blood-transfusion, administering compatible tested blood, at a dose rate of 22 ml/kg body weight, over the course of 4 hours, followed by the babesiicid therapy, using imidocarb dipropionate, at lower dose of 3 mg/kg b.w. (two intramuscular injections, 24 h apart). Additionally, supportive therapy was administered under permanent monitoring of the animal within the intensive care unit. Following the treatment, the dog’s status improved rapidly and clinically recovered within 10 days. This case-report describes a successful complex therapeutic protocol based on multiple approaches (blood transfusion, specific treatment, intravenous fluids and oral supportive treatment) for critical, life-threatening cases of canine babesiosis.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 1
Written by Cristian ZAHA, Raluca DASCALU, Larisa SCHUSZLER, Bogdan SICOE, Daniel BUMB, Cornel IGNA

Wounds that occur on the horse distal limb may evolve with the appearance of an exuberant granulation tissue that causes discomfort both to the animal and to the owner. Granulation tissue usually appears in the healing phase, but it evolves in excess due to the area prone to infection, with low blood supply, difficult to suture and in continuous movement. This case report describes the treatment of an exuberant granulation tissue in a mixed Romanian horse breed by histiotripsy using an elastic ligature. The success of the treatment consisted in gradual ablation of exuberant tissue given by the suppression of blood circulation, limited the local infection and subsequent stimulation of scarring. During the treatment, episodes of mild lameness and localized edema may occur, and in case of unexpected elimination of the exuberant mass a massive haemorrhage will occur.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVII, Issue 1
Written by Ana Maria GOANTA, Roxana IGNATESCU, Carmen IONITA, Natalia RADULEA, Lucian IONITA

This study demonstrates the utility of performing a relatively simple, inexpensive test during the diagnostic workup of several canine patients presented in the Internal Medicine Clinic of the Faculty of Veterinary Medicine of Bucharest. Although universally recommended, urinalysis is infrequently used in our clinic when not diagnosing and monitoring lower urinary tract disease. The cases presented will reinforce the necessity of performing a simple urinalysis and various pathologies that induce alterations of the urine, either in its chemical or cellular constituents or by interfering with the ability to concentrate urine. However, urinalysis is not diagnostic in every patient - it should be interpreted in light of the clinical presentation and of the other laboratory tests. The use of this test in the diagnosis of most nonurinary diseases requires serial examinations to emphasize persistent alterations and demonstrate the need for a diagnostic workup.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVII, Issue 1
Written by Raluca Mihaela TURBATU, Cristina FERNOAGĂ, Niculae TUDOR, Constantin VLĂGIOIU

The vestibular system is a sensory system comprised of several structures and tracts that is responsible for providing the brain information about spatial orientation and balance. Clinical signs like head tilt, nystagmus, positional strabismus or leaning are cardinal symptoms of a vestibular syndrome in small animal neurology. To establish an appropriate therapeutic plan and to provide owners accurate information regarding the prognosis, a differential diagnosis between a central or a peripheral vestibular disease is essential. In 2019, more than 310 cases of dogs and cats with neurological symptomatology were diagnosed in the Clinic of the Faculty of Veterinary Medicine in Bucharest, in agreement with the protocol already implemented in our practice. Full history, physical examination followed by a complete neurological examination were mandatory steps required to localise the lesion within the four main regions of the brain: forebrain, brain stem, cerebellum or vestibular apparatus. Among the total number of cases, approximately 15% showed clinical signs consistent with a vestibular syndrome. This article aims to present the predisposition factors, clinical features and neurological findings of the 32 dogs and 16 cats diagnosed with vestibular pathology.

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