Scientific Works Series C. Veterinary Medicine

PRINT ISSN 2065-1295, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVII, Issue 1
Written by Cristian IONICĂ, Maria Rodica GURĂU, Dragoș COBZARIU, Georgeta ȘTEFAN, Dana Mihaela CREȚU, Doina DANEȘ

Canine parvovirus infection is one of the most common diseases of puppies. Dogs are affected by two viruses of the Parvoviridae family: CPV-1 (Bocavirus genus), thought to have minimal pathogenic potential, but having being associated with different disorder in all age category dogs, and CPV-2 (Parvovirus genus), known as Canine Parvovirus, the true parvovirus. Despite the extensive use of vaccination, the prevalence of infection registers an oscillating dynamic, the virus is evolving and the requirement for the confirmation diagnosis is being continuous. The aim of the research was to identify the possibly correlations of the clinical manifestations with the results of CPV-2 detection, using the real time PCR. Fourteen dogs with clinical manifestations associated with the suspicion of canine parvovirus, has been tested on feces samples by real time PCR: 13 were positive (92.85 %) to the real-time PCR test. The registered positiveness has been associated with different Ct values ranging from 6.82 to 35. One sample (7.14%) was negative. In this study, despite the variation range of Ct's, the clinical pattern registered did not directly relate with the virus amount, rather with the age.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 2
Written by Ruxandra COSTEA, Ruxandra TUDOR, Andra DEGAN, Roxana TURCU, Cătălin IVAȘCU

Critically ill and emergency patients often need anesthesia and analgesia for examination, clinical procedures or surgery. Anesthesia must be performed based on advanced planning. Anesthesia for this group of patients is unpredictable and with rapid consequences. Critically ill patients are prone to have a low ability to maintain homeostasis and tissue oxygenation. Good outcomes relay on rapid identification, diagnosis and stabilisation. This study covers a general approach for anesthesia in emergency and critically ill patients and the major complications that can occur in the recovery period. Various aspects will be taken into consideration related with the anesthetized critical ill patient, anesthesia protocols and the most common complications.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVII, Issue 1
Written by Cristina FERNOAGĂ, Raluca Mihaela TURBATU, Alexandru Gabriel NEAGU, Tudor NICULAE, Constantin VLĂGIOIU

Myasthenia gravis is an immune-mediated disease that affects the neuromuscular junction due to the increased production of antibodies directed against the acetylcholine receptors of skeletal muscles. The consequence is the impairment of action potential transmission from nerve to muscles. Although in human medicine, Myasthenia gravis is a well-known condition with diagnostic protocols and several therapeutic strategies, in veterinary medicine diagnosis and therapy for this condition are still challenging. This study presents the case of a 5-year-old male American Staffordshire Terrier referral to the Clinic of Faculty of Veterinary Medicine in Bucharest for a neurological consultation due to a history of fatigue, limb tremor and reluctance to exercise. After the complete physical and neurological examination, the findings were consistent with Myasthenia gravis, so neostigmine methylsulfate was administered intravenously to confirm the diagnosis. Considering the positive clinical reply that was obtained after 15 minutes, a long-term therapeutic scheme was established and the patient was reassessed periodically.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVI, Issue 1
Written by Diana Mihaela ALEXANDRU, Ionuț Răzvan DOBRE, Maria CRIVINEANU

Canine osteosarcoma is an agressive tumour that has both a locally invasive and highly metastatic biologic behavior. Osteosarcoma is the most important primary bone tumour in dogs, being the most common. Usually, it's occurrence is in the appendicular skeleton and the most frequently involved are large and giant breeds, being the cancer of elderly, large dogs. The aim of this case report is to describe the diagnostic methods, the therapeutic targets and the evolution of a canine osteosarcoma. This case highlights that the diagnosis is made by corroborating the results of the blood tests, the imaging methods (radiographs, CT scans) and the cytological evaluation. Multimodal therapy should be considerd to provide patients with maximum survival time and to improve the quality of life. The therapeutic management for a canine osteosarcoma case should include surgery, adjuvant chemotherapy and palliative care. Despite these therapies, the prognosis is, for the most part, negative with a great metastatic occurrence, mostly to the lungs. This report is an example of how diagnostics and therapeutics can be used in the management of a canine appendicular osteosarcoma.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVI, Issue 2
Written by Laurenţiu Ionuţ ISPAS, Ionuț-Cristian GÂRJOABĂ, Alexandru-Gabriel NEAGU, Niculae TUDOR, Constantin VLAGIOIU

Vertebral column fracture surgery in dogs is used to correct severe cases of spinal deformity and spinal cord compression. In the last years more and more cases of vertebral column fractures or luxation in dogs by car accidents as our case are met with varying degrees of injury to the spinal cord. In our study we present a dog hit by a car, which presented clinical sign of vertebral column fracture with little ventro-lateral displacement and suspected of spinal cord compression at the level of T12-T13 with paralysis; this was confirmed by x-rays and Magnetic Resonance Imaging (MRI). The anesthesia used was an inhalation type. The surgery was made to stabilize and reposition the vertebral column through repositioning the two splitted parts of the vertebral column in the normal anatomic position using a dorso-lateral approach and a metallic plate with 3 screws adapted and modified especially for the patient and for the fracture difficulty’s needs. We concluded that the recovering was fast and with complete motion on the back part of the body due to rapid diagnosis and intervention even if the surgery came was performed five days later after the car accident and also with the help of the adjuvant therapy (vitamin B therapy, pain-killers, antibiotics, Myodine).

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 1
Written by Cornel IGNA, Roxana DASCALU, Bogdan SICOE, Cristian ZAHA, Larisa SCHUSZLER

Scapular luxation is an uncommon cause of forelimb lameness in cats. Dorsal dislocation of the scapula occurs by trauma, having a result a tearing of the muscular support for the scapula, the serratus ventralis, trapezius, and rhomboideus muscles. In this case report is presented the surgical management of dorsal scapula luxation in one adult cat. Dorso-caudal scapula approach (dissection of the infraspinatus muscle) was performed and a small portion of teres major muscle was elevated. Caudal scapular border was attached of the 5th rib with a cerclage wire and the torn edges of the trapezius and rhomboideus muscles were sutured. Postoperative pneumothorax was diagnosed and treated with thoracentesis and aspiration. Clinical outcome was considered good with resolution of lameness and normal scapular motion. Open reduction and internal fixation (scapulo-costal cerclage) represent one treatment option in dorsal scapular luxation in cats.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVII, Issue 1
Written by Teodor Ștefan IONESCU, Maria Rodica GURĂU, Dragoș COBZARIU, Stelian BĂRĂITĂREANU, Doina DANEȘ

The objective of the current study was to determine the immunological changes in canine blood donors in Romania. The data of the study is being collected since 2016 and is still in process of research. The samples are represented by serum antibodies IgG - Canine Parvovirus and IgG - Canine Distemper virus, which were analysed at each blood donation of dogs being part of the donation program. They fulfil the eligibility criteria of being clinically healthy with a completed vaccination schedule. The study is based on 17 canine blood donors (5 males/12 females), over one-year-old (2-4 years), with owners and living in similar environments. The ELISA VetLine Canine Distemper Virus (CDV) and VetLine Canine Parvovirus (CPV) kits from NovaTec (Immundiagnostica GMBH, Germany) were used following the kits manufacturer's recommendations. The analysis of the results obtained from the serum samples collected showed that no individual presented negative results (0/17-0%) below the protection standard on the two viral strains, all the serum samples having positive results (17/17-100%). The results indicate that repeated blood donation cannot influence the loss of post-vaccine antibodies.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVI, Issue 1
Written by Carmen Daniela PETCU, Emilia CIOBOTARU-PÎRVU, Oana Diana OPREA, Oana Mărgărita GHIMPEȚEANU

With the development of the food industry, the products have undergone different phases of diversification, so today we meet products that did not exist yesterday or simply were not known, referring, for example, to "BIO", "ECOLOGICAL" or "ORGANIC" products. These can be defined as products of animal or vegetable origin, obtained without the use of chemicals or genetically modified components, which have not been exposed to irradiation, and as a result of their production, the environment has not suffered. In this study, we analyzed samples of dairy products represented by drinking milk, sour-batter milk, kefir, fermented cream, yogurt and fruit yogurt. A comparison was made between conventional products and ecological products of the same type, in terms of ingredients, and their nutritional value. To identify the different features of conventional or ecological dairy products, physico-chemical parameters such as fat content, carbohydrates, proteins, salt or other added substances were analyzed. Regarding the verification of the ecological products labeling, in all the analyzed samples it was found that the ingredients used come from the ecological agriculture, therefore respecting the labeling requirements. At the same time, these products were analyzed organoleptically, observing the appearance, colour, consistency, smell and taste. Analyzing the obtained results, it was found that the ecological products show some changes in the chemical composition and nutritional values. The salt level of the dairy products analyzed, although it is described on the label as part of the natural salt of raw milk, in the case of ecological products, the value obtained is lower, compared to that of conventional products. Ecological fruit yoghurts have a higher content of piece of fruit compared to conventional yogurts. In conclusion, some ecological products have a higher nutritional value compared to conventional dairy products, without registering statistically significant changes.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 2
Written by François Djitie KOUATCHO, Djanabou MOUSSA, Koné MAMADOU, Ndraouni FRIKI L, Bobga AOUDOU, Pidotcho GOLOMTA, Alexis TEGUIA

The present study was carried out from April to May 2018 in order to evaluate the effect of feed supplementation by M. oleifera leaves meal (MOLM) on quail (Coturnix sp.) production performances in the sudano-guinean zone of Cameroon. For this purpose, 192 quails of 21 days old with similar live weight were divided into 12 batches of 16 birds (8 males and 8 females). To a feed containing 20.18% Crude Protein and 3013.78 kcal of M E, 0, 1, 2, or 3% of MOLM was added corresponding to treatments T0, T1, T2 and T3, respectively. Each of these experimental diets was randomly assigned to 3 batches in a completely randomized design with 4 treatments repeated 3 times each. Data were collected throughout the 5 weeks of trial on growth performances, carcass and some reproductive characteristics. At the 7 weeks of the trial, 2 males, 2 females per batch were sacrificed to evaluate carcass and some reproductive characteristics. The main results showed that regardless of sex, the significantly higher (224.13 ± 30.69 g) live weight was obtained with 2% of MOLM compared to the control (203.83 ± 16.67 g). The significantly higher (4.74 ± 0.93) feed conversion ratio was obtained with the T3 treatment compared to T2 (4.24 ± 0.80 g). Carcass yield of males ranged from 70 (T2) to 73% (T3) and from 67 (T2) to 69% (T0) in females. Only whole carcass yield, relative weights of the wishbones, neck and gizzard were significantly (P<0.05) affected by MOLM. First songs in males as well as the first egg were recorded at 6 weeks age with 3% MOLM, which also induced a higher (73.01%) egg laying rate than control batch (57.14%). Treatments supplemented with 2 and 3% MOLM increased testicular weight (4.63 ± 0.34g and 5.48 ± 0.46 g respectively) compared to the control (3.54 ± 0.73 g). It has been concluded that MOLM can be used up to 3% to improve growth performance of quail in the finishing phase. Also, a 3% supplementation could be used to improve reproductive performance.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVII, Issue 1
Written by Lucica SIMA, Teodora SUPEANU, Mihai Cristian MĂRGĂRIT, Mădălina CIOARIC, Rosalie Adina BĂLĂCEANU, Nicolae DOJANĂ

In this study, the effect of ejaculate sampling frequency on the morphological properties of sperm in Plymouth Rock roosters was determined. Four experimental groups were tested: of five, four, three, and two weekly ejaculates. The age of the roosters at the beginning of the experiment was 28 weeks and the duration of the experiment was 30 weeks. Increasing the frequency of semen sampling from one weekly sampling to five samplings per week had the following effects: 1. increase in ejaculate volume, but decrease in sperm density; 2. no significant changes in sperm motility; 3. increase of spermatozoa viability and 3. significant influences on the percentage of anomalies but no significant influences on the types of anomalies (the most common being the sperm head abnormalities).

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