Scientific Works Series C. Veterinary Medicine

PRINT ISSN 1222-5304, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 1
Written by Raluca Mihaela TURBATU, Cristina FERNOAGĂ, Niculae TUDOR, Constantin VLĂGIOIU

Encephalitis represents inflammation of the brain tissue and is a common disease that affects especially the small-breed dogs. For a proper diagnostic, the patient history accompanied by a general and neurological examination are important elements. If neurological signs specific for forebrain localization are present, then a suspicion of an inflammatory lesion should be taken into consideration for the differential diagnostic list. For encephalitis, the list of differential diagnostic includes meningoencephalites of unknown origin (necrositing meningoencephalitis, granulomatous meningoencephalitis, eosinophilic encephalitis) and infectious meningoencephalites (bacterial, fungal, rickettsial and viral). In 2018, more than 200 cases presenting neurological disorders were diagnosed in the Faculty of Veterinary Medicine of Bucharest. Considering the fact that encephalitis was a common diagnostic among these cases, this paper includes a presentation of predisposition factors and also clinical aspects that we used for a proper diagnostic approach.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 2
Written by Dragos-Constantin COMAN, Emilia BALINT, Nicolae MANOLESCU

It is know that cellular immunity system has an overwhelming implication in pathology, therefore, if it has persistent stimulate, for a long period of time, the cellularity of it can undergo a marked changes, from the blast cells proliferation until the appearance of atypia and cellular monstrosity, thus foreshadowing the installation of the worst plague of our century, cancer. Thus, chronic lymph node hyperplasies represent an anatomo-clinical manifestations of the borderline between benign and malignant, both histologic and clinic, involving, in the same time, the lymphocytic and reticulo-histiocytic cell lines. This chronic hyperplasia status may have a short-term evolution, ending with healing, but, in the same time, it may have a multi-relapse, wich, if is not treated, it will became a true malignant lymphoma. Starting from these lesions, in this paper, we realised an epidemiological study, to the slaughtered animals from swine species, regarding the incidence of chronic lymph node hyperplasia, the precursor of malignant lymphoma, reported to the pathology and location of adenopathies.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 1
Written by Cristin COMAN, Teodoru SOARE, Diana ISTRATE, Andrei STOIAN, Diana ANCUȚA Jasmina MANOLESCU

Guinea pigs are rodents still used for scientific purposes in experimental models for tuberculosis, diphtheria, vitamin C deficiency, etc. One of the tests to which the guinea pig is widely used is the in vivo test of antigenicity. In this test, the substances are inoculated intravenously onto a previously sensitized guinea pig and analyzed whether the products tested cause anaphylactic shock. Intravenous inoculation is done, according to procedures, 2 times at 7 days in the intravenous penile vein, therefore only males are used. The purpose of the study was to evaluate intravenous inoculation methodology and welfare of guinea pigs by assessing the reaction produced after 3-way intravenous injection, penile vein, auricular vein and safenous vein, in the antigenicity test. The article describes the intravenous inoculation methodology for each route, the clinical health status assessment and the histological evaluation of the inoculated veins. The results obtained show that the safenous vein represents a safe way of intravenous inoculation, both as a technique and as side effects and histopathological lesions, and can be an alternative to the current method.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVII, Issue 1
Written by Ruxandra COSTEA, Ovidiu ROȘU, Ioana ENE

This study was performed in order to evaluate an anaesthetic protocol used for remote chemical immobilization of ten free ranging feral horses (Equus ferus caballus) for the following purposes: translocation, health assessment and/or contraceptive immunization. Horses were immobilized using a Ketamine/Medetomidine combination delivered remotely with a 6 mL dart syringe from a dart gun. A mean (X̅) induction time of 7 minutes (SD = 2.82) with a X̅ = 83.3 minutes of recumbency was recorded using X̅ = 2.37 mg/kg Ketamine (1.89-2.81 mg/kg) and X̅ = 0.09 mg/kg Medetomidine (0.08-0.1 mg/kg). Heart rate (X̅ = 38.43), respiratory rate (X̅ = 26.14), rectal temperature (X̅ = 35.12), relative arterial oxygen hemoglobin saturation (X̅ = 83.11) and capillary refill time (1-1.5 s) were monitored during anaesthesia, every 10 minutes after induction until recovery. This protocol was suitable for the chemical immobilization of the feral horses from the Danube Delta - Romania providing a good anaesthesia depth and muscular relaxation. Nevertheless, supplementation of Oxygen is recommended for the resulting hypoxia.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVII, Issue 1
Written by Fabiola IONIȚĂ, Diana ANCUȚA, Cristin COMAN, Mario Darius CODREANU

The worldwide prevalence of obesity has risen dramatically in the last decades. Obesity is associated with multiple medical conditions that appear in metabolic syndrome such as: type II diabetes, increased blood pressure, high triglyceride and cholesterol levels. The aim of this study was represented by the evaluation of diet-induced obesity, with the purpose of creating an experimental mouse model for testing food supplements and medication used in this syndrome. One group of C57BL/6 mice received an in-house purified hypercaloric diet, the second group received a standardized obesity diet and the third group received a control diet for a period of 60 days. The following aspects were assessed during the experiment: food intake, body weight, hematologic and biochemical parameters. On the final day, organ samples were collected (liver, kidneys and visceral adipose tissue) for necropsy and histopathologic examination. The obtained results showed that the administration of the in-house purified hypercaloric diet for a period of 60 days was optimal for installing obesity syndrome in mice. The use of unidirectional enriched diets presents an increased interest in current research for futher development of new therapeutic strategies in metabolic syndrome.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVI, Issue 1
Written by Emoke PALL, Mihai CENARIU, Simona CIUPE, Ovidiu GRAD, Ioan Stefan GROZA

Platelet rich plasma (PRP) in combination with different type of biomaterials can promote tissue regeneration. Our study aimed to evaluate the adhesion, proliferation and chondrogenic differentiation potential of canine mesenchymal stem cells on functionalised collagen-based biomaterial. Characterized gingival mesenchymal stem cells (1x105) were seeded on PRP functionalized synthetic biomaterial and were cultured in DMEM/F12 culture medium supplemented with 10% fetal calf serum and 1% antibiotics-antimycotics. After 24, 72 h and 5 days cellular adhesion, proliferation was assessed using CCK8 assay. The absorbance was recorded using a a BioTek Synergy 2 microplate reader set at 450 nm. The bioactive potential of PRP functionalized biomaterial was evaluated after 21 days. Proteoglycans and sulfated glycosaminoglycan content were assessed. Statistical analysis was performed using GraphPad Prism 5.0 software and statistical significance was considered when p < 0.05. Our results indicated the potential of PRP on cells attachment, proliferation and due to the release of growth factors promote the cells differentiation.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVI, Issue 1
Written by Ruxandra COSTEA, Andrei IONESCU

Testing of a defibrillation protocol is necessary in order to add additional security to the monitoring and support protocols during the experimental procedures developed on pigs. Pigs are placed under general anaesthesia and continuous monitoring (capnography, pulse-oximetry, blood pressure measurement). The experimental testing consists in creating the conditions needed for the heart to enter the states of ventricular tachycardia (VT) and ventricular fibrillation (VF) followed by the pre-chosen defibrillation protocol. By every defibrillation shock added, the normal sinus rhythm was sustained for a longer period, in relation with the number of shocks applied. Survivability could not be sustained only with defibrillation, the use of CPR and cardio tonic medication being recommended.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVI, Issue 1
Written by Mihai CENARIU, Emoke PALL, Mihai BORZAN, Remus CHIOREAN, Ioan Stefan GROZA

Pregnancy rates obtained after artificial insemination following Ovsynch in lactating dairy cattle are currently around 40-45%. Therefore, various fertility programs have been established in an attempt to increase these percentages. The aim of the present research was to use a simple and cost-effective presynchronization protocol, that would allow maximization of ovulatory response after the first GnRH of Ovsynch, and therefore improve fertility of treated animals, in comparison to those in which Ovsynch was used alone. A total of 240 non-pregnant and lactating dairy cows were divided in two groups (n = 120). Group 1 was synchronized with Ovsynch alone, while in group 2, PGF2α and GnRH were administered almost simultaneously, in different injection sites, 7 days before the initiation of Ovsynch. Ovarian structures were observed by ultrasonography on the day when the first GnRH injection of Ovsynch was made and 4 days later. Results showed a 42.5% pregnancy rate in group 1 and a 57.5% pregnancy rate in group 2. Therefore, presynchronization treatments allowed an improved pregnancy rate of 15%, which totally covers the supplemental costs implied by such measures.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 2
Written by Florina RAICU, Constantin VLAGIOIU, Niculae TUDOR

If in a laboratory elemental analysis (heavy metals) on solutions is being performed, there are a couple of very efficient spectrometric techniques that can be used. Flame and Furnace Atomic Absorption (AA) is one of them. This article aims to highlight the applicability of both techniques (Flame Atomic Absorption Spectroscopy “FAAS” and the other one that is Graphite Furnace Atomic Absorption Spectroscopy “GFAAS”) that are being used in the analysis of animal and non-animal food samples. For obtaining a relevant pieces of information in order to help the analyst, it is useful to make a parallel between the two AA techniques. This parallel is obtained by objectively answering to a series of questions to make sure that the chosen technique is the best from different point of views (type and number of samples, the type of the analyzed element, the time allocated for each analysis etc.) The defining elements include understanding the operating principles of each mentioned technique (FAAS and GFAAS), their strengths and their weaknesses (spectral interferences, background effects, matrix effects).

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 1
Written by Elena Camelia GAVRILĂ, Neculai PATRICHE, Marian BOGOESCU, Dorin SORA, Mădălina DOLTU, Maria CRIVINEANU

The aquaponic sistem is giving by concomitant aplication of hydroponia (plant culture without soil) and aquaculture (culture of fish). To set up a aquaponic system more stages are necessary. The environment for aquaponic sistem should be attently chosen to avoid pollution factors. Taking in consideration cold regime in our country aquaponic system was settled down inside of micro greenhouse belogs to Institute of Research and Development for Processing and Marketing of Horticultural Products Bucharest. Fishponds were building using 0.8 mm OSB panel covered with polyethylene wrap. Water physico- chemical analyzes was performed as following: temperature, oxygen dissolved (DO), nitrate, nitrite, ammonia, calcium, magnesium and phosphorus. The studies performed showed the maintenance of aquaponic system.

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