THERAPEUTIC EFFICACY AND SAFETY EVALUATION OF ERYTHROCYTE CONCENTRATE USED IN DOGS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 2
Written by Ildikó BARABÁSI, Viorica CHIURCIU, Constantin CHIURCIU, Laurenţiu OGNEAN

We studied the clinical and hematological changes of 18 dogs from admission day (T0) until 5 days post-transfusion (5 days after the last administered transfusion), as well as hematocrit changes 6 hours post-transfusion therapy with erythrocyte concentrate. This research took place in a period of 6 months in 2014 in a small animal clinic from Germany. Most of the patients have been diagnosed with immune-mediated hemolytic anemia (n=11), 2 with rodenticide poisoning, 1 with babesiosis, 1 with hemangiosarcoma, 1 with septic peritonitis, 1 with idiopathic hepathopathy and 1 with hypothyroidism. The 18 patients received a total of 30 transfusions with erythrocyte concentrate in a mean dose of 11.46 ml/kg. Of the 30 transfusions, 6 reached the calculated hematocrit rise 6 hours post-transfusion, 2 had a higher than expected value and 22 did not reach the expected value. The hematocrit value 6 hours post-transfusion was statistically extremely significant (p<0.0001). We have observed positive changes in all hematological parameters 5 days after the transfusion therapy of which 3 have been statistically significant. The red blood cell count underwent a statistically very significant (p=0.0052) change, as has the hemoglobin level (p=0.0085). The hematocrit level had a statistically extremely significant change (p=0.0002) from the admission day until day 5 post-transfusion.

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BARABÁSI I., CHIURCIU V., CHIURCIU C., OGNEAN L. 2016, THERAPEUTIC EFFICACY AND SAFETY EVALUATION OF ERYTHROCYTE CONCENTRATE USED IN DOGS. Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 2, ISSN 2065-1295, 11-16.

TICK INFESTATION IN A MIGRATORY BIRD: A CASE REPORT

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Poliana TUDOR, Romanita MATEESCU

With this case report, we describe Dermacentor reticulatus ticks infestation in a Common Starling (Sturnus vulgaris) which was brought to our clinic by a citizen who involuntarily hit by car, in June 2012. The bird had massive infestation with ticks in the head and neck. Twenty-nine ticks were collected, and on the basis of the morphological criteria have been identified as Dermacentor reticulatus.

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Poliana TUDOR, Romanita MATEESCU 2013, TICK INFESTATION IN A MIGRATORY BIRD: A CASE REPORT. Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4, PRINT ISSN 2065-1313, 94-98.

TRANSGENIC PIGS FOR BIOMEDICAL APPLICATIONS

The purpose of this article is to review the most modern techniques used in obtaining transgenic pigs as important models in the study and understand of mechanisms involved in the pathogenesis of various human diseases. Genetically engineered animals have a significant role in biomedical research (Wheeler 2007, Robl et al., 2007, Vajta et al., 2012). Genetically modified swine are recognized as outstanding models for the study of various human diseases. These animals have high reproductive capacity and adequate precocity, short farrowing interval, parturition of multiple offspring (an average of 10–12 piglets per litter) (Wolf et al., 2000, Walters et al., 2012). Besides all these aspects the completed sequencing of the swine genome (Schook et al., 2005), made possible precise representations of human diseases based on propinquity of genes between the two species (Whyte, Prather 2011). Pigs are recognized as excellent models in a variety of areas, including nutrition, toxicology, dermatology, diabetes, cancer, atherosclerosis, cardiovascular disease, cutaneous pharmacology, ophthalmology, degenerative disease etc. (Lunney et al., 1999, Ishii et al., 2006, Herkenne et al., 2006, Dyson et al., 2006, Du et al., 2007, Matsunari, Nagashima 2009, Aigner et al., 2010, Noel et al., 2012, Prather et al., 2013). Due to the emergence of modern genetic engineering techniques, the genome of these animals can be modified to serve as a potential xenograft donor (Sachs, Galli 2009).

TULCEA PRACTICE CENTER – EDUCATION MEETING THE CULTURAL AND TOURISTIC SIDES

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Bogdan GEORGESCU, Gabriel PREDOI, Manuella MILITARU, Aneta POP, Letitia PURDOIU

After nearly a decade of lack of the component organized in practice centers in preparing students from the Faculty of Veterinary Medicine Bucharest, since 2009, this activity was revived by visits in the practice centers of groups accompanied by teachers with norm of practice. The study was conducted with three groups of 25 students and was held in the stages of the practice in 2010, 2011 and 2013. This practice activity offered the students the opportunity of applying knowledge acquired during the academic year/years. The groups of students who took the practice stage at Tulcea Sanitary Veterinary Department had the opportunity to perform various activities (baby boar castration, artificial insemination in sow etc.) on farms in the county. Students also actively participated in the maneuvers and activities of various departments of County Sanitary Veterinary Laboratory subordinated to SVD (necropsy, trichinoscopy, etc.). After completion of the program most students expressed their desire to visit different sights of Dobrogea. Thus, they had the opportunity to visit museums subordinated to Tulcea Eco-Museum Research Institute (Ecotourist Museum Center "Danube Delta" - Aquarium, Museum of History and Archaeology, Ethnography and Folk Art Museum), all of which are in the municipality area. On weekends, students were in boat rides on the Danube Delta canals, made equestrian tourism in Macin Mountains National Park, visited various archaeological sites and ancient and/ or medieval citadels (Aegyssus/ Tulcea, Noviodunum/ Isaccea, Enisala/ Heracleea, Histria, Argamum). The beautiful touristic journey included visits at Dobrogea Gorges and Gura Portitei. This approach to practical activity including the cultural side, respectively the touring side, had a very positive feedback from students who have completed the practice stage in an efficient and pleasant manner by an optimal combination of these sides with education.

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Bogdan GEORGESCU, Gabriel PREDOI, Manuella MILITARU, Aneta POP, Letitia PURDOIU 2013, TULCEA PRACTICE CENTER – EDUCATION MEETING THE CULTURAL AND TOURISTIC SIDES. Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4, PRINT ISSN 2065-1323, 145.

USE OF FRUCTOSAMINE IN SMALL ANIMALS WITH DIABETES

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Cristina FERNOAGĂ, Mario D. CODREANU, Mihai CORNILĂ, Raluca Teodora NAE, Radu CONSTANTINESCU

Diabetes mellitus in cats and dogs is a complicated illnes and its monitorisation is a chalLenge for the clinician. Thus, fructosamine indicates high level of blood glucose. The increased value of serum fructosamine is found in patients diagnosed with diabetes mellitus and it reflects the degree of glycaemic control, being useful for an objective and proper monitorisation. This parameter is much more accurate than the value of serum glucose level, especially when dealing with cats, due to the fact that in this type of patients the level of blood glucose can be affected by induced acute stress. This study includes 36 diabetic patients,19 dogs and 17 cats, from the Department of Internal Medicine of the Faculty of Veterinary Medicine Bucharest, in the past year, for which frutosamine has been determined. This has been conducted in order to asses quick changes in therapy and to improve glycaemic control.

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Fernoagă C., Codreanu M. D., Cornilă M., Nae R. T., Constantinescu R. 2015, USE OF FRUCTOSAMINE IN SMALL ANIMALS WITH DIABETES. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 99-102.

USE OF GUIDES FOR GOOD HYGIENE PRACTICE – A CERTAINTY FOR ACHIEVING SAFE AND HEALTHY FOOD: A REVIEW

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1
Written by Oana - Margarita GHIMPETEANU, Magda GONCIAROV

European Union states encourage preparing national guides to good hygiene practice and application of HACCP principles. It is encouraged the dissemination and use of national and Community guidelines. However, food business operators may use these guides on voluntary basis. Guides to good health practice must include adequate information on the risks involved in primary production and related activities and measures to combat such risks, including the relevant set of national and Community law or national and community programs. Using of the guides to good practice may help food establishments to control hazards and demonstrate compliance. The best practice guidelines are usually a combination of good hygiene practices (GHP) and HACCP- based elements and include: practice guidelines for the implementation of mandatory requirements; requirements for a raw materials; hazard analysis; pre-establishment of critical control points in the preparation and processing of food; preventive hygiene measures for handling sensitive and perishable products and prepared food for groups of consumers with increased susceptibility to illness, the need for documentation and records, protocols for data validation of shelf life of the product. Guides of good sanitary practice represent a simple, but effective mean to overcome difficulties that may arise in certain food establishments to implement HACCP procedures. National and community guides must provide guidance on good practice to combat health risks in primary production and related activities. General guide may suggest common hazards and controls of certain food activities and help the manager or the HACCP team in making food safety procedures or methods and appropriate record keeping.

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GHIMPETEANU O.M., GONCIAROV M. 2016, USE OF GUIDES FOR GOOD HYGIENE PRACTICE – A CERTAINTY FOR ACHIEVING SAFE AND HEALTHY FOOD: A REVIEW. Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1, ISSN 2065-1295, 88-90.

VIABILITY ASSESSMENT OF BOAR SPERMATOZOA USING FLOW CYTOMETRY

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Laura PARLAPAN, Simona CIUPE, EmÖke PALL, Mihai CENARIU, Iulia Maria BALACI, Sandu Nicolae PÎNDARU, Ioan Stefan GROZA

During the last decades a technique based on membrane permeability to fluorescent dyes has been developed in order to determine these parameters. The aim of this study was to determine the fresh boar sperm viability by flow cytometry using a triple stain protocol. Fresh boar semen samples were analyzed with FACSCanto II (BD Biosciences) system on the basis of Hoechst 33348, peanut agglutinin-fluorescein isothiocyanate (FITC-PNA) and propidium iodide (PI) staining. Flow cytometric provide an accurate and precise technique which allowed detection of four categories of sperm: live sperm with intact acrosomal and plasma membranes, live sperm with acrosome lesions and intact plasma membrane, dying sperm with damaged plasma membrane and intact acrosome, as well dead cells with damaged plasma membrane and acrosome lesions.

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Laura PARLAPAN, Simona CIUPE, EmÖke PALL, Mihai CENARIU, Iulia Maria BALACI, Sandu Nicolae PÎNDARU, Ioan Stefan GROZA 2013, VIABILITY ASSESSMENT OF BOAR SPERMATOZOA USING FLOW CYTOMETRY. Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4, PRINT ISSN 2065-1308, 73-75.

X-RAY EVALUATION OF PROXIMODISTAL ALIGNMENT OF CANINE PATELLA

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Cornel IGNA, Roxana DASCALU, Larisa SCHUSZLER, Daniel BUMB, Adelina PROTEASA

This study used radiographic techniques to evaluate the Insall Salvati index (ratio of the length of the patellar ligament to the length of the patella - L:P) to compare the verticality patellar position at small-medium and large breed dogs that were grouped in healthy dogs, dogs with patellar luxation and with cranial cruciate ligament ruptures. Except for the based on L:P ratio measurement at small-medium breed dogs and in large-breed there was no difference among the groups. In large large-breed dogs the LPL is associated with patella baja. No statistically significant differences of L:P ratio between dogs affected or unaffected with cranial cruciate ligament rupture are reported Further studies using other imaging techniques are required in small and large-breed dogs with patella luxation (LPL and MPL).

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Igna C., Dascalu R., Schuszler L., Bumb D., Proteasa A. 2015, X-RAY EVALUATION OF PROXIMODISTAL ALIGNMENT OF CANINE PATELLA. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 128-130.


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