SERUM BIOCHEMICAL AND HISTOPATHOLOGICAL EXAMINATIONS OF SOME TISSUES OF LAMBS WITH MUSCULAR DYSTROPHY IN VAN

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 2
Written by Serkan YILDIRIM, Kivanc IRAK, Handan MERT, Inci DOGAN, Nihat MERT

White muscle disease (WMD), is an important disease also known as 'muscular dystrophy’ in the lamb and calf. White muscle disease is the result of degeneration of skeletal and cardiac muscles in lambs. Lambs mostly affected with the congenital form either born death or die a few day after birth. The disease is a manifestation of lack of selenium, vitamin E or both. Van and surrounding villiages were visited and lambs with WMD examined. The lambs with 3-10 days of age were used as research materials. Necropsy and gross examinations was performed to all lambs. The blood samples were analyzed for Vitamin E amount, Creatine kinase (CK), Aspartate aminotransferase (AST) and Lactate dehydrogenase (LDH) activities. The level of Vitamin E was decreased, but the other parameters significantly increased. In heart, chest and gluteal muscle lesions in lambs were found. Tissue samples were histopathologically examined. Muscular dystrophic calcification in necrotic areas as well as hyaline degeneration and Zenker necrosis were determined. In the calcified region mononuclear cell infiltration mainly macrophages, were observed. In conclusion in lamb with white muscle disease; the activities of enzymes related to muscle health were raised drastically. In gluteal, chest and especially in heart muscle the hyalin and Zenker degeneration were noted.

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YILDIRIM S., IRAK K., MERT H., DOGAN I,, MERT N. 2016, SERUM BIOCHEMICAL AND HISTOPATHOLOGICAL EXAMINATIONS OF SOME TISSUES OF LAMBS WITH MUSCULAR DYSTROPHY IN VAN. Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 2, ISSN 2065-1295, 52-58.

SKIN GRAFTS USED IN THE RECONSTRUCTIVE SURGERY OF SKIN WOUND WITH SEVERE TEGUMENTARY DAMAGE IN DOG

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Jacqueline MOCANU, Andreea Bianca BOFAN, Cătălina Anca CUCOȘ

Wound healing is a complex and dynamic process of replacing devitalized and missing cellular structures and tissue layers after injury. Wound healing is achieved through four precisely and highly programmed phases: hemostasis, inflammation, proliferation, and remodeling. For a wound to heal successfully, all four phases must occur in the proper sequence and time frame. After an severe trauma to the extremities of the limbs the skin necrose detaches as flaps remaining in place a large denuded area exposed to infection.The reduced mobility of the skin in these areas cause difficulty in healing process to occur complicating traumatic sequelae and functional impotence. The vicious scars are unsightly and often painful due to connective tissue contractility The purpose in wounds reconstruction using skin grafts is to complement dermal with denuded skin tissue surface and initiate an uniform healing a result of which the affected limb to resume the functionality. In this paper we present modalities ok skin grafts by heterotopic grafting and autografting with cutaneous flap pedicled

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Mocanu J., Bofan A. B., Cucoș C. A. 2015, SKIN GRAFTS USED IN THE RECONSTRUCTIVE SURGERY OF SKIN WOUND WITH SEVERE TEGUMENTARY DAMAGE IN DOG. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 153-155.

SPERMOGRAMS ANALYSIS AS A MANDATORY PART OF THE CANINE SEMEN CRYOPRESERVATION PROTOCOLS

Quality of fresh semen is a crucial element for the succes of assisted reproduction techniques in all species. 57 ejaculates were collected manually from 25 dogs. Motility and concentration (CASA SpermVision®, Minitübe, Germany, morphology and acrosome status (Spermac® stain) were determined. Semen analysis showed a strong relationship between male’s weight and size (testes dimensions) and the volume of the sperm rich fraction, but did not identify a correlation between concentration and male’s size. Motility parameters, morphology and acrosome status for the ejaculates included in our study were within the accepted standard values. Age did not significantly influence the volume or the morphology of canine semen.

STAPHYLOCOCCI RESISTANT PHENOTYPES OF THE INTERMEDIUS GROUP ISOLATED FROM DOGS

Dogs have frequently infections with coagulase-positive and coagulase-negative staphylococci with different locations. A total of 49 samples consisting of pathological skin, auricular and genital secretions were bacteriologically examined using standard methodology. The isolates were tested by disk diffusion Kirby-Bauer method, on which were used biodiscs with 19 antibiotics from different groups. The staphylococci strains isolated from dogs included in Intermedius Group were susceptible to the antibiotics rarely or not used in the therapy of diseases in this species. The isolates were methicillin-resistant strains, thus emphasizing the movement of these strains in the canine population, confirming the zoonotic risk of these strains. After this study several resistant phenotypes of staphylococci strains included in Intermedius Group were identified, whose frequency was variable.

STRONGYLE MONITORING IN A FLOCK OF THE NATIVE ZERASCA SHEEP BREED

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1
Written by Lorella GIULIOTTI, Maria Novella BENVENUTI, Francesca PISSERI, Simona NARDONI, Andrea MARTINI, Francesca MANCIANTI

Gastrointestinal parasites compromise the welfare and health of ruminants on pasture, causing serious productive losses. The constant and preventive strategy of anthelmintic treatments results in several problems, such as parasite resistance and food and environment contamination. Sustainable approaches to tackle such problems primarily involve knowledge of the dynamics and impact of parasites in the flock. The aim of this study was to monitor the gastrointestinal parasite burden together with the body condition score (BCS) in a flock of a local Italian sheep breed. The two-year study involved an unchanged sample of 20 pluriparous ewes randomly selected in a farm located in the homonymous area. Chemical anthelmintic treatment had been administered three months before the beginning of the study, following a mean infestation level of 298±276.5 eggs per gram (EPG). Faecal samples were collected every two months to evaluate the faecal egg count (FEC) with a modified McMaster technique. Egg dynamics were statistically analysed and data were logarithmically transformed to normalize the variance. FEC results were grouped into four classes of infection for a statistical description. The BCS was measured on a five-point scale. Results showed a significant fluctuation in FEC (from 52 to 320 EPG), however no clear relationship with the season was found. Mean values were always under the threshold of health risk and only in one case did values exceed 300 EPG. The overall BCS was nearly 3, thus revealing no nutritional problems. The study highlighted that by monitoring gastrointestinal strongyles in a farm with good farming practices, chemical treatments can be limited to only those cases that are strictly necessary.

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GIULIOTTI L., BENVENUTI M.N., PISSERI F., NARDONI S., MARTINI A., MANCIANTI F. 2016, STRONGYLE MONITORING IN A FLOCK OF THE NATIVE ZERASCA SHEEP BREED. Scientific Works. Series C. Veterinary Medicine, Vol. LXII, Issue 1, ISSN 2065-1295, 65-68.

STUDIES ON THE SUPPLY AND PRICE MANAGEMENT IN VETERINARY PHARMACEUTICAL UNITS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Valentin NICORESCU, Alexandra NEAGU, Maria CRIVINEANU, Camelia PAPUC, Corina PREDESCU

Nowadays, veterinarian’s activity takes place in a highly dynamic and changing environment; thus, the managers of economic units having as main activity the sale of veterinary products should have leadership and organizational qualities that allow them to develop a successful business in a competitive environment. The purpose of this study was to highlight the main aspects related to veterinary pharmaceutical units supply, and the manner in which price management is done. The study was conducted in 25 veterinary pharmaceutical units (pharmacies and pharmaceutical points) in Bucharest and consisted of a market survey in order to identify the factors that influence supply, the criteria by which suppliers are selected, rhythm and mode of supply, as well as aspects related to stocks’ management and control, pricing criteria, markup and its influencing factors. The results showed that the supply of veterinary pharmaceutical units is typically performed daily or weekly and is influenced by season, prices and profitable offers from suppliers. In an overwhelming percentage, veterinary pharmaceutical units’ stocks are electronically managed by using software that facilitates staff’s work, and most of the surveyed veterinary pharmaceutical units keep a safety stock. The most important factors that influence price levels are mainly represented by the competition, discounts, offers and benefits from suppliers, and the most common markup for veterinary pharmaceutical units is between 20-50%. Following the studies, it was concluded that modern management and marketing principles related to the supply, stocks and prices are properly applied in the surveyed veterinary pharmaceutical units

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Nicorescu V., Neagu A., Crivineanu M., Papuc C., Predescu C. 2015, STUDIES ON THE SUPPLY AND PRICE MANAGEMENT IN VETERINARY PHARMACEUTICAL UNITS. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 75-82.

STUDY ON ANTICANCER ACTIVITY OF DEUTERIUM-DEPLETED WATER (DDW) IN EXPERIMENTAL ONCOLOGY

The authors demonstrated the effect of deuterium depleted water (DDW) with a concentration of 60 ppm to be a very good inhibitor of the neoplastic cell proliferation in outbred Wistar rats inoculated with two strains of the highest tumor aggressiveness (Walker 256 and T8 Guérin). For this experiment were organized groups, in equal parts by sex of outbread Wistar rats, with an average weight of 100g, which were established both control groups and experimental groups. Through the experiment, animals were monitored in anatomoclinical terms. Laboratory tests were executed periodically, especially cytomorphological. Dead animals were subjected to a careful pathological examination. It is well known that high malignant Walker 256 and T8 Guérin tumour strains develop a solid, ulcerated subcutaneous cancer, after an incubation of 5-6 days. The reproductibility of this type of cancer is of 95%. Therefore the death of the rats occurs within a short period of 40-60 days. The cumulative effect of DDW 60 ppm on the rats grafted with Walker 256 and T8 Guérin strains was about 28-30 %. This percentage comprises both the animals in which the effect of primary reject of the tumour graft was noticed and the healing effect after an important development of the tumour.

STUDY ON THE MICROSCOPE METHOD AND THE VIEW OF THE ANALYTICAL TECHNIQUES FOR IDENTIFICATION AND ESTIMATION OF THE PROCESSED ANIMAL PROTEINS IN ANIMAL FEED

Hypothesis universally accepted as the most likely route of infection with ESB appeared due to the consume of the animal by-products which are not intended for human consumption that contained prion protein derivative - infected led to ban the feeding of farmed animals with processed animal protein (PAP) that focused primarily feed for ruminants and later expanded to all feed for all farm animals. Entry into force of the ban on the use of the processed animal proteins (PAP) in feed for farmed animals and especially in ruminants is considered an important measure of prophylaxis to prevent BSE so the identification and the microscopic estimation of the constituents of animal origin became the official method and mandatory in all Member States. The microscopic analysis depends upon the identification of the hsitological characters macro-and microscopic structural of the processed animal tissue added in feed mixtures.To identify the microscopic animal constituents, some technical conditions are essential: optical microscope, stereo microscope, high-density solvent (chloroform or tetrachloroethane) clarifying agents (phenol-glycerol, paraffin), microscope with digital visual images support as decision support . The method allows the identification of bone fragments, muscle tissue, hair, feathers, shell fragments and plant and mineral components. Nowadays four different approaches are applied to control the compliance on the prohibition of feeding with PAP: microscopic analysis, immunological analysis, infrared spectroscopy and microscopy (NIR), polymerization chain reaction (PCR). In this stage, the microscopic method is the only method validated and able to identify the nature of the animal in feed components with detection limit of <0.1%, but it cannot accurately detect the species of origin.

STUDY REGARDING ENDOPARASITES INFESTATION IN DOGS FROM HOUSEHOLD IN MORENI – DAMBOVITA AREA

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Al. NEAGU, Poliana TUDOR, C. VLAGIOIU

The objective of this study was to assess the prevalence of endoparasitic infestation in dogs. The study was performed on 155 samples of dog feces collected from dogs in households in Moreni – Dambovita area during the period July 2012 to June 2013. After microscopic examination, a prevalence of 72,25 % (112/155) was established, with identification of the following species: Ancylostoma caninum 60,71 % (68/112), Trichocephalus vulpis 45,53 % (51/112), Uncinaria stenocephala 23,21 % (26 /112), Toxocara canis 8,92 % (10/155), Toxocara leonine 7,14 % (8/155). In 61.60 % (69/112) of positive cases one parasitic species was indentified and in 38,39 % (43 /112) two parasitic species were indentified.The investigation had shown a high prevalence of endoparasitic infestation in dogs, studied area indicating a potential zoonotic risk.

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Al. NEAGU, Poliana TUDOR, C. VLAGIOIU 2013, STUDY REGARDING ENDOPARASITES INFESTATION IN DOGS FROM HOUSEHOLD IN MORENI – DAMBOVITA AREA. Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4, PRINT ISSN 2065-1305, 59-62.

STUDY REGARDING THE USE OF TETRACYCLINES IN THE TREATMENT OF CHICKENS AND THE IMPLICATIONS OF ACTIVE SUBSTANCE IN FOOD SAFETY

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXI
Written by Lucian-Ionel ILIE, Ovidiu SAVU, Manuella MILITARU

The correlation between food and the population health, they have led to an increasing demand for the chicken and chicken products, as a possible successfully alternative to the dissatisfactions offered by other food categories. In this study, the samples were collected from chicken, which were administered various veterinary products based on antibiotics, as a treatment of different diseases. The chickens they were slaughtered after the waiting period mentioned by the manufacturer in the leaflet. The samples were represented by muscles, liver, kidney and gastrointestinal mass and they were analyzed for levels of oxytetracycline, chlortetracycline and tetracycline. After the results integration, the samples analysis has not revealed the presence of these compounds. The study confirmed the need to use the antibiotics only for therapeutic purposes and to having the obligation to complying the time waiting to remove the active substance from the chicken body.

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Ilie L. I., Savu O., Militaru M. 2015, STUDY REGARDING THE USE OF TETRACYCLINES IN THE TREATMENT OF CHICKENS AND THE IMPLICATIONS OF ACTIVE SUBSTANCE IN FOOD SAFETY. Scientific Works. Series C. Veterinary Medicine, Vol. LXI, ISSN 2065-1295, 209-210.


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