Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 1
Written by Alexandra PETEOACA, Niels A.J. CREMERS, Iuliana IONASCU, Andrei TANASE

Lower extremity injuries in cats can present significant challenges by a lack of tissue, bone exposure, and bacterial contamination, requesting effective treatment methods. This case report describes the use of a combination of negative pressure wound therapy (NPWT) and medical-grade honey (MGH) dressing in the treatment of a complex lower extremity injury in a cat. An old injury in the metatarsal area of a 3-year-old male cat resulted in an open fracture of all the metatarsal bones. The wound was left open after osteosynthesis via intramedullary pin insertion. A combination of NPWT and MGH dressing was used to promote healing and prevent bacterial contamination. The combination of NPWT and MGH dressing was effective in promoting the healing and full closure of the soft tissue injury in this case. NPWT supported granulation tissue formation, maintained a moist wound environment, and prevented infection. The MGH dressing helped prevent further bacterial contamination and promoted wound healing. This case highlights the benefits of using a bimodal staged approach in the management of challenging lower extremity injuries in cats.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 1
Written by Ruxandra COSTEA, Ruxandra PAVEL, Gina GIRDAN, Ioana ENE, Florin POSASTIUC, Catalin MICSA, Tiberiu CONSTANTIN, Dorin TOGOE, Alexandru DIACONESCU

A 6-year-old Tsurcana male sheep was anesthetized in hospital conditions for a soft tissue surgery- bilateral orchidectomy. After intramuscular premedication with midazolam (0.2 mg/kg), butorphanol (0.1 mg/kg), and ketamine (5 mg/kg), anesthesia was induced intravenously with alfaxalone (1.6 mg/kg IV). The subject was intubated with an 8.5 mm endotracheal tube and anesthesia was maintained with isoflurane (1.5-2.5 vol % in O₂) and 3 boluses of alfaxalone (1.0 mg/kg) delivered intravenously every 10 minutes. The total surgical time was 40 minutes and the whole inhalation anesthesia time was 78 minutes, including the preparation of the subject at the beginning and after the surgical procedure. The mean arterial blood pressure was maintained at 80 mmHg throughout anesthesia, the average end-tidal CO₂ at 51 mm Hg, the mean oxygen saturation of haemoglobin was 99.5%, the mean heart rate was 130 beats per minute, respectively 31 respirations per minute. In conclusion, this anesthetic protocol may be clinically applicable for a male sheep undergoing invasive soft surgery, especially if there is a particular case involving a risk patient.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 1
Written by Anamaria Ioana PAȘTIU, Maria-Carmen TURCU, Dana Liana PUSTA

More than a half of bird species around the world are monomorphic, therefore they do not show distinct sexual dimorphic traits. The paper aims to present three molecular methods for sex identification of monomorphic companion birds. Samples of feathers, oral swabs and blood were collected from Psittaciformes (Ara ararauna, Psittacus erithacus and Psittacula krameri) and Columbiformes (Columba livia domestica). All samples were tested by three different PCR protocols in order to identify the chromo-helicase-DNA-binding (CHD1)-W gene and CHD1-Z genes in females and the CHD1-Z gene in males. Two protocols were conventional PCR, using P2 and P8, respectively P2 and NP primers, while the third protocol was a multiplex PCR using P0, P2 and P8 primers. As a conclusion, all three PCR protocols can be used for molecular sexing of monomorphic Psittaciformes and Columbiformes companion birds. Feather, oral swab and blood samples provided adequate DNA templates for the sex identification of birds.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 1
Written by Emanuel MITREA, Mariana IONITA, Kurt PFISTER, Ioan Liviu MITREA

Anaplasmosis, a tick-borne disease affecting cattle worldwide caused by Anaplasma spp. (Rickettsiales: Anaplasmataceae), is associated with considerable economic losses to dairy and beef industries. Data about exposure to Anaplasma spp. infection of cattle in Romania is limited. Therefore, a cross-sectional serological study was conducted to preliminary investigate the serological status on Anaplasma spp. infection in Romanian catlle. For this, 182 catlle originated from three counties in Northeastern and Southeastern Romania were included in the study. Cattle serum samples were tested with a competitive enzyme linked immunosorbent assay (cELISA) detecting an epitope of major surface protein 5 (MSP5) of Anaplasma spp. The results were expressed as percentage (%) of inhibition (I) calculated according to the indications of the manufactures; samples with I ≥30% were considered positive. Subsequently, the overall seropositivity was 42.3% (95% CI: 35.03 - 49.84); significant difference of seroprevalence values (p< 0.05) were registered according to the cattle originating county, ranging from 22.2% (95% CI: 13.26-33.57) to 72.0% (95% CI: 57.50-83.77). These findings emphasize on the need for further studies to identify the species and associated risk factors for Anaplasma inffection of cattle in Romania, as base for developing control strategies.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 1
Written by Cristin COMAN, Diana-Larisa ANCUȚA, Fabiola IONIȚĂ, Andrei Alexandru MUNTEAN, Costin Ștefan CARACOTI, Ioana Viorela CARACOTI, Cerasela DRAGOMIRESCU, Mircea Ioan POPA

Neisseria meningitidis (Nm) is the pathogen carried asymptomatically in the nasopharynx but which under certain conditions can produce meningitis or even multi-organ failure. Experimental induction of meningitis in an animal model is necessary for testing new nanopharmaceutical products. Thus, the aim of the study was to establish the concentration of Nm capable of inducing the disease. We carried out 2 studies where we tested different concentrations of Nm obtained by the nephelometric and spectrophotometric methods. In the first study we included 20 BALB/c mice, and in the second 24. Local and general clinical signs, complete with body temperature values, body weight, hematological, microbiological and histopathological examination were important indicators for the assessment of the establishment of meningitis. The clinical results have pronounced depression in the first 2 days after inoculation, then the general condition will appear. The microbiological and histopathological examination indicated the presence of bacteria at the brain level and specific meningeal lesions only in the case of the second attempt, but the early mortality, requires additional testing of the bacterial concentration that induces meningites in a stable and persistent way so that treatment against the condition can be tested later.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 1
Written by Marian GHIȚĂ, Ana ROTARU, Carmen Daniela PETCU, Simona NICOLAE, Oana Diana MIHAI, Gabriel COTOR

The aim of the present research paper was to evaluate the main electrocardiogram parameters in goat. The electrocardiograms were performed on a batch of 20 Carpathian goats, using the Dubois lead system. By analysing the results obtained, we concluded that, using the Dubois lead system, the best method of performing the electrocardiogram in goats is using lead II, the amplitude values being 0.117 mV for the P wave, 0.310 mV for the ventricular complex and 0.386 mV for the T wave, while lead I is not suitable for the EKG exam in this species, due to the extremely low waves’ amplitude, resulting in an almost obscure recording. Regarding the duration of the electrocardiographic waves, the values obtained were 0.048±0.016 seconds for the P wave, 0.046±0.014 seconds for the QRS complex and 0.088±0.016 seconds for the T wave. As for the segments and intervals, the durations obtained were 0.062±0.020 seconds for the P-R segment, 0.246±0.014 seconds for the Q-T interval, 0.384±0.037 seconds for the P-T interval and 0.096±0.020 seconds for the T-P segment. The heart rate, electrographically calculated, was 128.5 bpm ± 12.215.

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SECONDARY CARDIAC INVOLVEMENT OF MEDIASTINAL T-CELL LYMPHOMA IN A YOUNG SCOTTISH FOLD CAT - CASE REPORT

Written by Laura DARIE, Elvira GAGNIUC, Alice RADULESCU, Ana Simina MIHAI, Andreea Diana GONTOIU, Emilia CIOBOTARU-PIRVU
Pages 13 to 18

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RESEARCH ON THE VALUES OF SOME ELECTROCARDIOGRAPHIC PARAMETERS IN GOAT RECORDED USING DUBOIS LEADS

Written by Marian GHIȚĂ, Ana ROTARU, Carmen Daniela PETCU, Simona NICOLAE, Oana Diana MIHAI, Gabriel COTOR
Pages 19 to 24

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STUDY REGARDING THE DISTRIBUTION OF THE CELIAC ARTERY IN NEWBORN CALVES

Written by Sorina-Andreea MIHAI, Cristian BELU, Iulian DUMITRESCU, Bogdan GEORGESCU, Petronela- Mihaela ROȘU, Anca ȘEICARU, Gabriel PREDOI
Pages 25 to 30

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THE AGE IMPACT ON THE URINARY BEHAVIOUR IN CATS - COMPARATIVE CASE STUDY

Written by Simona NICOLAE, Iuliana CODREANU
Pages 31 to 36

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PCR PROTOCOLS FOR MOLECULAR SEXING IN MONOMORPHIC BIRDS

Written by Anamaria Ioana PAȘTIU, Maria-Carmen TURCU, Dana Liana PUSTA
Pages 37 to 40

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MORPHOLOGICAL PARTICULARITIES OF THE ZONOSKELETON, STYLOPODIUM, AND ZEUGOPODIUM OF THE THORACIC LIMB IN THE EURASIAN BROWN BEAR (Ursus arctos arctos) - CASE STUDY

Written by Petronela Mihaela ROȘU, Bogdan GEORGESCU, Cristian Romeo BELU, Iulian DUMITRESCU, Paul George STOICULEASĂ, Adela Ioana MUSTĂȚEA, Sorina Andreea MIHAI
Pages 41 to 48

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EFFECT OF LONG-THERM VITAMIN A AND E DIET SUPPLEMENT ON SOME SEMEN TRAITS IN ROOSTER

Written by Diana ȘIPOTEANU, Mădălina CIOARIC, Ivona ZĂBAVĂ, Maria Rodica GURĂU, Nicolae DOJANĂ
Pages 49 to 56

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PARTIAL INTRAVENOUS ANESTHESIA WITH ISOFLURANE AND ALFAXALONE FOR AN ADULT SHEEP UNDERGOING SOFT TISSUE SURGERY

Written by Ruxandra COSTEA, Ruxandra PAVEL, Gina GIRDAN, Ioana ENE, Florin POSASTIUC, Catalin MICSA, Tiberiu CONSTANTIN, Dorin TOGOE, Alexandru DIACONESCU
Pages 57 to 60

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STUDY ON TICK INFESTATIONS OF SMALL RUMINANTS, IN SOUTHERN ROMANIA

Written by Elisa Florentina DASCĂLU, Mariana IONITA, Ioan Liviu MITREA
Pages 61 to 67

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EFFICIENCY OF SURFACE DISINFECTION BY NEBULIZATION USING CUBE ATOMIZERS IN A VETERINARY UNIT – PRELIMINARY STUDY

Written by Catalina Valeria GARBACEA, Emoke PALL, Mihai CENARIU, Ioan Ștefan GROZA
Pages 68 to 73

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USE OF DIODE LASER IN OPHTHALMOLOGY SURGERIES IN DOGS AND CATS: 161 CASES (2019-2022)

Written by Iuliana IONAȘCU
Pages 74 to 87

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CLINICAL MANIFESTATIONS OF ACUTE PANCREATITIS IN DOGS - DIAGNOSTIC AND PROGNOSTIC VALUE

Written by Lazarin LAZAROV
Pages 88 to 92

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STUDY OF AN EPISODE OF SUBCLINICAL KETOSIS IN A SHEEP FARM IN SOUTHERN ROMANIA

Written by Adrian MIHAI, Roxana Mariana IGNĂTESCU (ȚÎMPĂU), Nicoleta Andreea MINCĂ, Carmen IONIȚĂ, Raluca Mihaela TURBATU, Lucian IONIȚĂ
Pages 93 to 97

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THE LONG TERM USE OF ENFLICOXIB IN DOGS WITH OSTEOARTHRITIS: CLINICAL SAFETY AND EFFICACY

Written by Eleonora MONTI, Giulia MORETTI, Rolando ARCELLI, Alexandra PETEOACA, Lisa GAROFANINI, David FORTI, Antonello BUFALARI
Pages 98 to 109

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HUMAN LACTOFERRIN CAN ENHANCE THE OSTEOGENIC DIFFERENTIATION OF EQUINE MESENCHYMAL STEM CELLS?

Written by Emoke PALL, Mihai CENARIU, Simona CIUPE, Cristian CRECAN, Ioan Stefan GROZA
Pages 110 to 115

[Abstract][Full article][Citation]

PRELIMINARY DATA ON DETECTION OF ANTIBODIES AGAINST Anaplasma spp. IN CATTLE, ROMANIA

Written by Emanuel MITREA, Mariana IONITA, Kurt PFISTER, Ioan Liviu MITREA
Pages 116 to 120

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NEGATIVE PRESSURE WOUND THERAPY AND MEDICAL-GRADE HONEY COMBINATION SWIFTLY HEALS A LOWER EXTREMITY INJURY WITH BONE EXPOSURE IN A CAT

Written by Alexandra PETEOACA, Niels A.J. CREMERS, Iuliana IONASCU, Andrei TANASE
Pages 121 to 129

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CANINE RHABDOMYOSARCOMA - LITERATURE REVIEW

Written by Adina-Mihaela PÎRVU, George-Laurențiu NICOLAE, Manuella MILITARU
Pages 130 to 136

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SPECIFIC THERAPEUTIC MANAGEMENT IMPLICATIONS IN NEONATAL LAMBS MORTALITY

Written by Stere SPONTE, Alexandra Mihaela CRISTIAN, Mario CODREANU, Iuliana CODREANU
Pages 137 to 140

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DIAGNOSTIC APPROACH TO BRAINSTEM DYSFUNCTION IN DOGS AND CATS - A CASE SERIES REPORT

Written by Raluca Mihaela TURBATU, Cristina FERNOAGĂ, Alexandru Gabriel NEAGU, Roxana-Mariana IGNĂTESCU (ȚÎMPĂU), Constantin VLĂGIOIU
Pages 141 to 145

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THORACIC WALL RECONSTRUCTION WITH POLYPROLENE MESH IN A DOG WITH SEVERE FLAIL CHEST

Written by Seralp UZUN, Iuliana IONASCU, Dragos-Marian DUMITRESCU, Tiberiu Sebastian IANCU, Catalina GEORGESCU, Dumitru MAGALEAS, Radu JERCAU, Georgeta FILIP, Ada IONESCU
Pages 146 to 151

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THORACIC TRAUMA UPDATES IN FELINE HIGH-RISE SYNDROME. WHAT CHANGED IN 30 YEARS? 50 CASES IN ONE YEAR

Written by Seralp UZUN, Iuliana IONASCU, Florin DUMITRESCU, Dragos-Marian DUMITRASCU, Tiberiu Sebastian IANCU, Radu JERCAU, Catalina GEORGESCU, Dumitru MAGALEAS, Georgeta FILIP, Ada IONESCU
Pages 152 to 162

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DETERMINATION OF CHEMICAL-ANALYTICAL QUALITY CRITERIA OF KING SCALLOP (Pecten maxima), ATLANTIC SEA SCALLOP (Placopecten magellanicus) AND QUEEN SCALLOP (Aequipecten opercularis) SAMPLES FROM NORTH ATLANTIC OCEAN, ENGLISH CHANNEL AND FROM THE TRADE MARKET

Written by Cătălina Nicoleta BOIŢEANU, Nicoleta CIOCÎRLIE, Florin NEACSU, Laurențiu TUDOR
Pages 163 to 174

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IDENTIFICATION OF ANTIBIOTIC RESISTANCE PATTERNS IN Escherichia coli BACTERIA FROM CLOACAL SWAB SAMPLES OF BROILER CHICKENS FROM FARM THAT USE PROBIOTIC Lactobacillus sp.

Written by Daniswara Danindra DARMESTI, Roostita L. BALIA, Gemilang Lara UTAMA
Pages 175 to 181

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THE USE OF HISTOLOGICAL METHODS IN MEAT AND MEAT PRODUCTS FOR FRESHNESS DETERMINATION - A POSSIBLE FUTURE TREND?

Written by Raluca-Aniela GHEORGHE-IRIMIA, Manuella MILITARU
Pages 182 to 188

[Abstract][Full article][Citation]

THE WORLD OF MYCOTOXINS - A SYSTEMATIC REVIEW

Written by Ioana POROȘNICU, Luminița-Iuliana AILINCĂI, Mihai MAREȘ
Pages 189 to 198

[Abstract][Full article][Citation]

PRELIMINARY RESULTS IN INDUCING MENINGITIS IN BALB/C MICE USING A HUMAN STRAIN OF Neisseria meningitidis

Written by Cristin COMAN, Diana-Larisa ANCUȚA, Fabiola IONIȚĂ, Andrei Alexandru MUNTEAN, Costin Ștefan CARACOTI, Ioana Viorela CARACOTI, Cerasela DRAGOMIRESCU, Mircea Ioan POPA
Pages 199 to 205

[Abstract][Full article][Citation]

EFFECT OF ISOFLURANE ANESTHESIA ON THE VITAL SIGNS MONITORING IN LABORATORY MICE

Written by Fabiola IONIȚĂ, Cristin COMAN, Mario CODREANU
Pages 206 to 212

[Abstract][Full article][Citation]

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 1
Written by Laura DARIE, Elvira GAGNIUC, Alice RADULESCU, Ana Simina MIHAI, Andreea Diana GONTOIU, Emilia CIOBOTARU-PIRVU

Mediastinal lymphoma in cats affects the lymph nodes in the cranial mediastinum and/or the thymus. The tumor comprises 10-20% of feline lymphomas. Males appear to be overrepresented. A 2-year-old neutered male Scottish Fold cat with hypertrophic cardiomyopathy treated according to the results of the medical investigation was presented for a second opinion consult. X-ray examination showed a gigantic neoplastic mediastinal mass, and the ultrasound examination revealed a right-sided deviation of the heart axis. Due to the poor prognosis, the cat was euthanized, and the necropsy was performed. Mediastinal T-cell lymphoma with neoplastic invasion into the pericardium and discreetly into the epicardium was diagnosed based on gross pathology, histopathology, and immunohistochemistry. The left atrium presented endocardial fibroelastosis, while the ventricular myocardium showed intercellular edema, myocardocyte laceration, vacuolar and hyaline degeneration, and necrosis in small, isolated areas. Additionally, the lung displayed diffuse collapse, edema, and congestion.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 1
Written by Stere SPONTE, Alexandra Mihaela CRISTIAN, Mario CODREANU, Iuliana CODREANU

Curative interventions, expensive and long, are often followed by clinical recovery, but with compromise and reduction of weight dynamics and productive growth. In this context, the precocity of the therapeutic approach, together with the consistency and dynamic modulation of the treatment of peri- and neonatal diarrhea syndromes in lambs conditions the recovery of such affected patients (with minimal productive consequences), contributes to ensuring the well-being of the animals and reducing the risk of inducing antibiotic resistance. The research took place between 2022-2023 on 196 lambs of different breed and age, within the county of Tulcea, the locality of Baia. The animals included in the study were divided into 2 groups, namely study group 1 consisting of 98 lambs in which the diagnosis was established clinically and study group 2 consisting of 98 lambs that benefited from a definite etiological diagnosis (rapid Rainbow test Bio K) along with an etiologically oriented and specific therapeutic protocol. The percentage analysis of the mortality rate in the diarrheal syndrome in lambs shows an increased value in lambs treated with non-specific medication compared to lambs in which the therapeutic protocol approached was of an etiological nature where the percentage value is reduced. The calculated productive difference in body weight between etiologically diagnosed and related treated group 4 versus group 1 without etiotropic approach resulted in a 15% greater weight gain at day 60 in the group 4 member compared to the group 1 member.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 1
Written by Adrian MIHAI, Roxana Mariana IGNĂTESCU (ȚÎMPĂU), Nicoleta Andreea MINCĂ, Carmen IONIȚĂ, Raluca Mihaela TURBATU, Lucian IONIȚĂ

Subclinical ketosis in sheep is considered a disorder of carbohydrate metabolism, which causes the mobilization of lipids, disturbance of fatty acid metabolism and the formation of ketone bodies. The main objective of this work is to identify local and general factors that contribute to the occurrence of subclinical ketosis in a sheep farm in Dolj County (Southern Romania) and to highlight by laboratory analysis the disturbances of energy metabolism, as well as other conditions closely related to them. A good nutritional management of the ewes before and after lambing is essential knowing that their nutritional requirements increase in this period and the peak will be reached in the first two-three weeks of lactation. Careful monitoring of the ewes in this period: trained personal to identify early signs of disease, diagnostic tests and quick intervention is also needed to prevent these episodes and other complications, such as pregnancy toxemia, which can lead to severe nervous manifestations (secondary to hypoglycemia) and significant economic losses.

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