Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 1
Written by Anamaria Ioana PAȘTIU, Maria-Carmen TURCU, Dana Liana PUSTA
More than a half of bird species around the world are monomorphic, therefore they do not show distinct sexual dimorphic traits. The paper aims to present three molecular methods for sex identification of monomorphic companion birds. Samples of feathers, oral swabs and blood were collected from Psittaciformes (Ara ararauna, Psittacus erithacus and Psittacula krameri) and Columbiformes (Columba livia domestica). All samples were tested by three different PCR protocols in order to identify the chromo-helicase-DNA-binding (CHD1)-W gene and CHD1-Z genes in females and the CHD1-Z gene in males. Two protocols were conventional PCR, using P2 and P8, respectively P2 and NP primers, while the third protocol was a multiplex PCR using P0, P2 and P8 primers. As a conclusion, all three PCR protocols can be used for molecular sexing of monomorphic Psittaciformes and Columbiformes companion birds. Feather, oral swab and blood samples provided adequate DNA templates for the sex identification of birds.
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