Scientific Works Series C. Veterinary Medicine

PRINT ISSN 2065-1295, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Belu C., Predoi G., Georgescu B., Dumitrescu I., Anca Șeicaru , Petronela Roșu , Carmen Bițoiu

In according to species, forearm bones have a different topography and development in conjunction with the use of the hand. When mobile they can rotate one around the other to perform pronation and supination movements. Otherwise, they are welded, radius being more developed than ulna. Study was to describe motfologiei articular surfaces in mammals, including humans, linking data obtained with pronation and supination possibilities of autopodium.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Mihaiu Liora, Mihaiu Marian, Alexandra Lăpuşan, Dan Sorin, Romolica Mihaiu, Carmen Jecan, Ionuţ Cordiş

The antimicrobial resistance of the most probable pathogen germs isolated from raw meat has not been tested thoroughly yet in our country. The transmissibility of this resistance from food to human has been previously described in the foreign literature that is why such a complex study is mandatory given the current situation. For the isolation and antimicrobial resistance assessment, the classical method was used, the confirmation being performed through molecular methods (simplex PCR). In the antimicrobial evaluation the automatic system TREK was used. The majority of the Escherichia coli strains isolated from the meat samples were confirmed as non-pathogenic ones but revealing a high number among them as being resistant to ampicillin and enrofloxacin. The Salmonella spp. bacteria isolated was found in a lower amount and with a high prevalence of resistance to cefazolin, cefuroxime and tetracycline. Although a lot of food poisoning episodes are treated in the infectious diseases hospitals with ampicillin, enrofloxacin and tetracycline it has been shown in this study that these antibiotics might not have the wanted effect. We recommend the antibiogram in every case given the fact that these bacteria have become more and more resistant due to improper use of antibiotics in animal feed, animal illnesses and human diseases.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Lăpuşan Alexandra, Mihaiu Liora, Mihaiu Marian, Dan Sorin, Romolica Mihaiu, Ionuţ Cordiş, Dorina Dragomir

Although the tonsils removal at pigs is mandatory in conformity with the European Union Regulation 854/2004, there are slaughtering units that do not respect this rule. The importance of their complete removal is shown in this study through a thorough assessment of the bacteria load found in tonsils and afterwards in the carcasses where the tonsils have not been removed. For the isolation of these bacteria the classical method was used and the confirmation being performed through biochemical microtest systems (API) and molecular methods (simplex PCR). Also, there were two other automatic ways of bacteria identification: the microscan WALKAWEY system and the Trek system. The results revealed a polymorphic microflora, with a predominance of Gram negative bacteria in the majority of the tonsil samples examined. The bacteria prevalence in the pharyngeal tonsils was represented by: Gram negative bacilli (32.1%), diplococcic (19.75%), streptococci (16.05%), Gram positive bacilli (13.58%), polymorphic non spore forming Gram positive bacilli (8.64%), spore forming Gram positive bacilli (1.24%) and Candida (8.64%). Following the bacteriological exam, a large variety of bacteria species were revealed at the carcasses also, worth mentioning are Staphylococcus, Proteus, Streptococcus, Listeria and Salmonella and the prevalence of these species was significantly higher (p<0.05) during the warm season than the cold one in both units studied. The pharyngeal tonsils at pig represent a deposit area for some pathogen bacteria (Yersinia enterocolitica, Staphylococcus aureus, Escherichia coli, Salmonella spp.), that can contaminate the carcasses during slaughtering and can jeopardize the consumers’ health.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Ş. Tiţă, C. Savu, Anca (Popescu) Dobrea, O. Savu

During the period 2009-2011, 3971 meat samples and meat preparations collected both from agri-processing units and from alimentary cold stores and food marketing network were analysed within the Sanitary-Veterinary Laboratory of Dolj County, thus seeking to isolate and identify the serotypes of Salmonella spp. incriminated in triggering food poisoning. Of the total number of samples examined, six samples were positive representing 0.15 percent. The positive samples were composed of pork minced meat (3), a mixture of prepared beef and pork meat (cattle-swine) (1), refrigerated minced pork meat rolls (1) and turkey wings (1). All strains of Salmonella spp. isolated in the Sanitary-Veterinary Laboratory of Dolj County were submitted for serotyping of relevant isolates of Salmonella spp in the reference laboratories within the Institute for Diagnosis and Animal Health (IDSA) and the Institute of Hygiene and Public Veterinary Health (IISPV). Four strains belong to the Salmonella Typhimurium serotype, one strain belongs to Salmonella Goldcoast and one strain belongs to Salmonella Hadar. The dominant serovariant identified among tested isolates is Salmonella Typhimurium (66.33 %). Of the four serovariants of isolated Salmonella Typhimurium, three serovariants presented the classical antigenic structure and one serovariant exhibited a different antigenic structure, lacking the 1,2 factors – initially denominated as Salmonella Typhimurium-like; it has been recently denominated as Monophasic Salmonella Typhimurium.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Chiurciu Viorica, Tudoran C, Diaconu Lucia, Stoica C, Iacob I, Raduta Maria Mioara

This paper presents data obtained from investigations made in pig farms with intensive growing system and from households, the presence and distribution of immunosuppressive pathogens circulating in swine populations in Romania (PRRS, M. hyopneumoniae, Circovirus, etc.) and their immunosuppressive influence on the effectiveness of immunoprophylaxis programs, applied in pig farming. The benefit of obtaining such data is to obtain and apply some effective immunoprophylaxis methods in order to limit economic losses and increase the food quality and safety. The influence of the mentioned germs on the anti-erysipelas immune response has taken into account because this vaccination is a currently made action applied in the current technology of growing pigs. Preliminary results showed that there is some influence of studied immunosuppressive microbial agents including the association germs on the effectiveness of immunoprophylaxis programs applied in breeding pigs.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Razvan-Marius Vlagioiu, Gabriela Chioveanu, Nicolae Cornila, Florica Barbuceanu

In order to identify structural modifications produced on the intestinal epithelium of the bee, consecutive with sporozoa Nosema spp parasitism, examination studies have been performed for fixing and histological coloration steps of anatomical pieces gathered from medium and posterior intestine of these species.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Anca-M. Galiş, Laura M. Dale, Christelle Boudry, André Théwis

In a context of high productivity, eggs’ quality assessment is necessary for enhanced safety and quality assurance towards the consumers and feedback for producers. The quality assessment of eggs and egg products is performed using destructive and time-consuming methods, therefore the use of rapid tools becomes mandatory, especially in the case of a high production rate. Near Infrared (NIR) spectroscopy is considered a very reliable and rapid technique with large use in food industry. At the farm level, NIR spectroscopy technique would be an interesting tool to determine the chemical and physical properties of eggs, eggshell and internal quality and, moreover, this information may help the layer farm manager when a problem occurs in the flock. Its application in the egg industry is aimed at the quality changes in eggs during storage and quality assessment of the egg products, through the compositional analysis. It is possible and in some cases successful the prediction and/or determination of different parameters such as: protein, total lipid and total solids content (for liquid egg products), polyunsaturated fatty acids (for freeze-dried egg yolk), moisture, fat and protein content (for spray-dried whole eggs). In addition, for the white colored shell eggs, the detection of blood and meat spots is also successful. Further studies with NIR and near infrared hyper spectral imaging system (NIR-HSI) are needed in this direction, as the results obtained until now are very promising for the development of a rapid tool for quality assessment of eggs and egg products.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Anca Alexandra Dobrea (Popescu), Constantin Savu, Mimi Dobrea, Ileana Păunescu, Gabriel Murariu

In this work the sensitivity of two Lactobacillus salivarius strains isolated from dental root canal (G1 and G2) and two Lactobacillus probiotic strains by intestinal origin: Lactobacillus salivarius probiotic and Lactobacillus rhamnosus GG, against different antibiotics has been examined. All of them were found to be very sensitive (Ø ≥25mm) to rifampicin and penicillin, whereas, all were resistant to acid nalidixic and streptomycin. These data show that cultures should be tested for their antibiotic sensitivity before being used as probiotics or in food industry.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Coman Dragos-Constantin, Manolescu Nicolae, Balint Emilia

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Molnar Maria Eugenia, Falcă Constantin, Petruse Cristina

The experiment was conducted on 144 Isa Brown hens, for the duration of a production cycle. The biological material used in the experiment was divided into 4 experimental groups, each group being constituted of 36 hybrid hens. In the experiment we used a structure of mixed supplemented fodder in three experimental variants with selenium, zinc and magnesium. The blood biochemical parameters were determined in the three experimental stages. In the ascending phase of the egg laying curve, proteinemia ranged below the lower limit reference with the hens in the control group and those in the group supplemented with magnesium. In the steady phase proteinemia was in reference values at all experimental variants. In the downward phase although proteinemia was within reference limits, significant differences were confirmed between the control group and the groups supplemented with minerals. In the upward and downward phase of the egg laying curve, enzymatic activity of SGOT was within reference limits in all groups of hens, and in the steady phase, its activity was greatly reduced in the groups of hens supplemented with minerals. In the upward phase SGPT had lower values for all the hens in the experiment. In the downward phase its activity was higher in hens in the control group compared to the groups supplemented with minerals. In the upward and downward phase the enzyme activity of GGT was within normal limits, only in the steady phase it was more intense. ALP showed higher values for all hens in the experiment.

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