Scientific Works Series C. Veterinary Medicine

PRINT ISSN 2065-1295, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Constantin Lupescu, Adrian Vasile, Rares Popa

Official controls should be carried out through appropriate techniques therefor, including checkups and a more intensive controls such as inspections, verifications, audits, sampling and sampling controls. Community rules should not apply to primary production for private domestic use or preparation, handling and storage of food for private domestic consumption. Relevant risk assessment takes into account the probability of their occurrence, severity and reproduction at every stage technology. Food business operators shall develop, implement and maintain a permanent procedure or procedures based on HACCP principles.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Laurenţiu Tudor, Anca-M. Galiş, Elena Mitrănescu, Aneta L. Tudor

Listeria monocytogenes remains one of the many foodborne pathogens that require continuous monitoring. The detection and tracing methods are quickly developed for a better improvement and a quicker identification of this foodborne pathogen that may be present in food samples. A total number of samples consisting of different types of food products were collected from local markets in Bucharest, subjected to enrichment steps (according to EN ISO 11290-1 and ISO 11560) and further on to a PCR analysis. This resulted in eight samples with positive results, among them goat cheese, smoked fish, sausages and raw chicken meat. The identification was possible and quick, no matter the bacterial load of the samples, due to the existence of a virulence gene pertaining to the internalin gene family: InlB, which is specific to L. monocytogenes. This method ensures a high sensitivity and specificity in a very short period of time, reducing the work time for this analysis with five days concerning the negative results and seven days for the positive ones.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by I. R. Dobre, Silvia Oana Dobre

This paper highlights the importance of diversifying teaching methods and learning techniques in practical training in veterinary medical field, aiming a more efficient instructive and educational process. This study was performed using the methods: investigation and psycho-pedagogical experiment on groups of internships students in livestock units, veterinary laboratories and food processing units. Within the scientific approach were investigated several teaching and learning methods and techniques: brainstorming, brainwriting, case study , simulation , reciprocal teaching and learning and stellar explosion. After applying these methods and techniques, it was observed a significant progress in understanding and assimilation of content and better transposition of the theory in practice. Interactive teaching strategies promote active learning, the students organized in small groups are working together and colaborating, to achieve and realize their objectives. All teaching-learning investigated methods can be used successfully in the practical training of veterinary medical students.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by N. Dojană*, Iuliana Codreanu, Claudia Preda

Three groups of adult rabbits were fed for 35 days with high starch diet (high starch group, HSG), high protein diet (high protein group, HPG), or high fat diet (high fat group, HFG) compared with a control group (CG) fed with a specific diet. Then pancreatic juice was collected and measured in acute experiments, in two variants of secretion: basal and stimulated by secretin. Pancreatic juice samples were analyzed for protein content and amylase, trypsin and lipase activities. Basal values of juice flow showed no significant differences between any experimental fed group vs. CG (P>0.05). Secretin stimulated juice flows were increased in all the groups, but the increase was significant higher only in HSG vs. CG (P<0.05). Basal protein flows of experimental fed groups did not differ significantly vs. CG (P>0.05). In contrast, the stimulated protein output was significant higher in HSG vs. CG (P<0.05). Amylase activities were significant higher in HSG vs. CG, both in basal (144.3×103 and, respectively, 52.0×103 amylase units (AU), P<0.001) and in the stimulated pancreatic juice (422.0×103 and, respectively, 162.1×103 AU, P<0.001). Moreover, the activities of trypsin and lipase in HSG did not differ significantly vs. CG (P>0.05), nor for basal neither for stimulated juice. Trypsin activity (in nmols benzoylargynil- ethyl-ester decomposed / 10 min / kg b.w.) increased significantly in HPG vs. CG, both in basal (62.5 vs. 22.2, P<0.01) and in stimulated juices (166.0 vs. 31.5, P<0.001). On the other hand, amylase and lipase activities of HPG group were similar to those of CG. Basal lipase activity (in mequivalents of liberated oleic acid per mg protein per h, 37°C) was higher in HFG vs. CG (122.4 and 86.5, respectively). In the stimulated juice, lipase activity increased to 246.0 in HFG and 184.1 in CG, but no significant differences were found in HFG vs. CG nor for lipase neither for amylase and trypsin (P>0.05).

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Anca Alexandra Dobrea (Popescu), Constantin Savu, Mimi Dobrea, Iuliana Gâjâilă

In vitro researches were carried out for evaluate specific growth rate and generation time of two Lactobacillus salivarius strains isolated from dental root canal and two Lactobacillus probiotic strains at pH values 4.5 and 7.0. These tests indicated that Lactobacillus salivarius strains isolated from dental root canal showed biger specific growth rate values at both pH values compared with the probiotic Lactobacillus strains.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Rares Popa, Adrian Vasile, Constantin Lupescu

Units that produce, process, store, transport and / or distribute products of animal origin may carry out veterinary checked only after obtaining the veterinary authorization. Before entering the legal representative catering units required to obtain, where appropriate, the veterinary authorization, conditional authorization or authorization veterinary intra-community trade in food of animal origin from the Veterinary Direction and Food Safety. This unit gives authorization for the activities concerned only if the food business operator has demonstrated that the unit complies with the relevant requirements of Regulations (EC) no. 852/2004 and (EC) no. 853/2004 and other relevant requirements of food law. Establishments handling products of animal origin may perform activities only if they have been approved by the competent authority. After rectification of deficiencies, the legal representative of the unit applies for veterinary direction and food safety county reevaluation unit.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Ipate Iudith, Bogdan A.T, Seregi Janos, Gottfried Brem, Constanta Strasser

Adaptation to climate change is essential for any efforts to promote food security, poverty alleviation, or sustainable management and conservation of natural resources. Many countries are already dealing with climate change impacts. The dynamic of food‘s world population projection to 2050 year and consumption dynamic of cereals, animal products (meat, milk, eggs) in terms of respect international standards of food safety and security; the known european principles “from the farm to the fork” and “from the farm to the plate” which have restricted rules established by European Food Safety Authority, must be respected in agrifood products 2050 year perspective also. In this framework, the food safety and security must be correlated with the respect of known principles of Hazard Analysis and Critical Control Points, based on actual international standards from ISO 9001-9002 series (Quality Management System), ISO 14001: 2004 (Environment Management), ISO 22000 (Food Safety). The projections for the future socio-economic environment and the assessment of the situation and prospects of the natural resource base raise the question as to whether and under what conditions the estimated future food demand can be met and how food security can be achieved.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Curcă Dumitru, Ioana Cristina Andronie, Viorel Andronie

The union of the young veterinary surgeons who decided to establish the Scientific Medical Society of Romania, at the Veterinary School of Bucharest. The “Statute of the Scientific Medical Society from Romania” drawn in the first meeting of 15 May 1871, were voted in the general assembly of August 14-16, 1871, chaired by Mauriciu Colben. The Scientific Medical Society didn’t have its own journal upon establishment. Therefore, in 1879, Alexandru Locusteanu, Mihai Măgureanu, Gheorghe Perşu and Panait Constantinescu, publish the Veterinary Surgeon. Succeeding to dismiss some difficulties, 33 veterinary surgeons met on 10-12 May 1882 in Bucharest for the First Congress of Veterinary Medicine, the first of this kind in Romania. The topics of the congress, set in 1881, included the following subjects: sanitary police, animal husbandry, public hygiene, pathology, miscellanea. Once the Society of Veterinary Medicine was relaunched, the Journal of Veterinary Medicine, Animal Husbandry, Hygiene and Rural Economy also appeared. It has been established in 1888 too, by I. Şt. Furtună, and had an Annex, the Bulletin of the Society of Veterinary Medicine. These meetings debated the important problems of the veterinary pathology confronting that period: the foot and mouth disease, of horses glanders, tuberculosis, the bovine gastro-entero-nephritis (babesiosis) etc. The Society existed until July 21, 1949, when, after this last meeting, it was discontinued abusively by the communist regime. Because the centenary of the Romanian Society of Veterinary Medicine was closing, a group of teaching staff from the Faculty of Veterinary Medicine of Bucharest started in the spring of 1970, the activities necessary to resume the Romanian Society of Veterinary Medicine: it was re-established on May 15, 1971, 22 years after its discontinuation and one hundred years after its foundation.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Călin V., T. Petruţ

Batches of piglets reared in intensive system were nonspecific immunomodulated During the experiment, three blood samples, necessary for haematological determinations,have been performed. In group A, it was administrated a bacterial suspension (Corynebacterium parvum). Group B received Levamisol product (for veterinary use) and group C received vitamin E and Selenium, using Romselevit. Group D was used as a witness group, being submissed to vaccination only. Hemoglobin concentration in group A (modulated with Corynebacterium parvum), significantly increased after the second harvest compared to harvest I. The final collection showed a significant decrease in these concentrations, all distinctly significant compared to harvest II. In groups B, C, and D, hemoglobin concentrations showed an increase in statistical terms ,only at an intermediate collection (highly significant), then remained constant.In group A, modulated with Corynebacterium parvum, hematocrit increased significantly distinct from harvest I. The final collection showed a decrease in these levels, manifested statistically significant from the second harvest.In groups B and D, hematocrit increased statistically at the intermediate harvest (significant) The final harvest was similar to the intermediate concentrations. In group C there were changes in the sense that after a distinctly significant increase in hematocrit values ,it decreased at the intermediate harvest without any interest in statistical terms. The number of red blood cells showed a distinctly significant increase in group A,at the second collection compared to the first one ,the final harvest decrease being statistically significant.

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Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Ilie L.I., Tudor L., Mitrănescu Elena, Galiş Anca-Maria

In this study we analyzed the evolution of technological parameters of goats carcass according to age. For this study we created two groups of samples, namely: group I represented by the goatling and group II represented by adult goats. On these meat samples we analyzed: meat chemical reaction (pH), water holding capacity, cooking loss test and drip loss (losses from refrigeration). For this samples we analyzed a total of 30 samples of goat meat for 15 for each group considered to be studied. Samples were collected from goats slaughtered during 2012 in a slaughterhouse in SE Romania. The values for the chemical reaction of the first group samples (goatling) varied between 6,20 and 6,32, the average being 6,26 and for the group II between 6,31 and 6,39, with an average of 6,35. Water holding capacity showed an average value of 61,23% for group I to 64,17% for group II. Cooking loss are recorded 37,62% to group I to 32,28% in group II, and for drip loss 4,06% for young kids to 4,88% to adult samples. Results lead to the conclusion that slaughtering goats at a younger age represents a disadvantage for manufacturing and processing, generating qualitative and quantitative losses in finished products.

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