SEROSURVEILLANCE OF NEOSPORA CANINUM IN FARM AND COURTYARD CATTLE

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Violeta Enăchescu, Mariana Ioniță, Ioan Liviu Mitrea

Neospora caninum is an apicomplexan parasite related to Toxoplasma gondii, well known as an important cause of abortion in cattle. In live animals the routine diagnostic of infection is detection of anti-N. caninum antibodies in serum. This study aimed to investigate the exposure to N. caninum infection in cattle from south area of Romania, and to survey the seroprevalence in some herds. A total of 542 sera from dairy cattle (16 herds in 9 counties) were sampled at different intervals. In the first sampling only farm animals were included, but in the second sampling courtyard cattle were added (n=47). Anti-N. caninum antibodies were detected using an indirect ELISA test. Anti-N. caninum antibodies were found in all herds. A total of 189 samples (34.9%, CI95%=30.85-39.05) were positive, with 104/258 (40.3%, CI95%=43.27-46.58) in the first sampling and 85/284 (29.9%, CI95%=24.66-35.63) in the second sampling. The highest rate of infection was in Giurgiu County, with 18/29 infected animals (62.1%). Prevalence increased with age and abortions were more frequent in seropozitive caws (9/74, 12.2%) than in seronegative ones (5/83, 6%). Seroprevalence was significantly higher in farm (37.2%, 184/495) than in courtyard cattle (10.6%, 5/47). At the second investigation in the same farms prevalence decreased significantly, compared with the first one (35.1%, 46/131 vs. 49%, 47/96). N. caninum infection is widespread in dairy farms from the studied area and may be a cause of abortion in some herds. The prevalence decreased at the second investigation in the same farms.

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Enăchescu V., Ioniță M., Mitrea I. L. 2012, SEROSURVEILLANCE OF NEOSPORA CANINUM IN FARM AND COURTYARD CATTLE. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4, PRINT ISSN 1222-5304, 136-145.

STUDIES ON THE THERAPY WITH GONADOTROPIN-RELEASING HORMONE (GnRH) AND HUMAN CHORIONIC GONADOTROPIN (hCG) IN GENITAL DISORDERS IN BITCHES

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Mihai Cristian Popescu, Maria Crivineanu, Mario Darius Codreanu

Infertility in bitch is characterized by a variety of clinical manifestations depending on the disorders of reproductive system. The purpose of this study was to asses the efficacy of gonadotropin-releasing hormone (GnRH) and human chorionic gonadotropin (hCG) in the treatment of prolonged estrus caused by follicular cysts in bitch. In this study, there were diagnosed 10 bitches with prolonged estrus having as etiology the presence of follicular cysts. The intensity of clinical signs was studied and vaginal smears were performed, resulting in high percentages of keratinized cells in bitches with follicular cysts. Determinations of estrogen and progesterone hormones were made, the presence of follicular cysts being suspected based on the high values of estrogen and duration of estrus. Duration of estrus signs ranged between 32-76 days, progesterone concentration was between 1.3-3.4 ng/ml, while the concentration of estrogen hormones ranged between 141.5-379.5 pg/ml. Based on the high values of estrogen hormones, the presence of keratinized cells, the duration of estrus and the age of bitch it was suspected the existence of follicular cysts. The efficacy of hormonal treatment (GnRH and/or hCG) was monitored by determining the values of estrogen and progesterone hormones, the results being correlated with the interpretation of vaginal smears and clinical manifestations. Following hormonal treatments, in 7 bitches clinical signs of estrus disappeared in 4-6 days, while 3 bitches still presented the clinical manifestations characteristic to estrus phase after the treatment.

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Popescu M. C., Crivineanu M., Codreanu M. D. 2012, STUDIES ON THE THERAPY WITH GONADOTROPIN-RELEASING HORMONE (GnRH) AND HUMAN CHORIONIC GONADOTROPIN (hCG) IN GENITAL DISORDERS IN BITCHES. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4, PRINT ISSN 1222-5304, 316-321.

STUDY CONCERNING ECTOPARASITES INFESTATION IN DOGS AND CATS IN THE TÂRGOVIŞTE-DÂMBOVIŢA AREA

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Mateescu Romaniţa, Tudor Poliana, Mateescu C.

The objective of this study was to determine the prevalence of ectoparasites infestation in dogs and cats, and their type in the Târgovişte-Dâmboviţa area. In the period September 2011 - September 2012, 685 dogs and 180 cats were examined. A prevalence of ectoparasites from 52.41% to 51.67% in dogs and cats was identified. The following species of ectoparasites were identified in dogs: Ctenocephalides canis – 33.71%, Ctenocefalides felis – 7.24%, Rhipicephalus sanguineus – 24.51%, Dermacentor reticulatus – 11.42%, Trichodectes canis – 16.99%, Demodex canis – 7.52%, Sarcoptes scabiei var canis – 4.18%. While the following species of ectoparasites were identified in cats: C. felis – 88.17 %, Otodectes cynotis – 12.90%, Rh. sanguineus – 6.45%, Felicola subrostratus - 2.15%, Microsporum canis – 2.15%. It was found that 5.57% of patients had multiple infestations in dogs, and in cats 12.90%. To our knowledge, this is the first report on infestation with Rh. sanguineus in cat in the Târgovişte-Dâmboviţa area.

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Mateescu R., Tudor P., Mateescu C. 2012, STUDY CONCERNING ECTOPARASITES INFESTATION IN DOGS AND CATS IN THE TÂRGOVIŞTE-DÂMBOVIŢA AREA. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4, PRINT ISSN 1222-5304, 262-271.

STUDY CONCERNING THE URINARY TRACT DISEASES IN CATS IN THE DÂMBOVIŢA COUNTY

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Mateescu C., Tudor N., Mateescu Romaniţa, Vlăgioiu C.

The results of the examination of a number of 198 of cats with urinary tract diseases in the Agervet-Târgovişte clinic during 2012 are presented. The sick animals were both mixed breeds (69.19%) as well as pure breeds (30.81%), aged between 1 and 15 years (with an average of 7.53 years). The urinary tract diseases had an acute evolution in 62 cats (35.96%), and in 136 cats (64.04%) they had a chronic evolution. Cystitis (58.08%), urolithiasis (56.06%), nephritis (30.80%), pyelonephritis (11.62%), renal cysts (1.5%) were diagnosed. The clinical manifestations were represented by: dysuria (63.13%), periuria (51,52%), pollakiuria (49.49%), stranguria (43.94%), hematuria (39.90%), anorexia (32.83%), vocal signs (31.31% ), vomiting (16.16%), pyrexia (29.29%), hypothermia (23.23%).

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Mateescu C., Tudor N., Mateescu R., Vlăgioiu C. 2012, STUDY CONCERNING THE URINARY TRACT DISEASES IN CATS IN THE DÂMBOVIŢA COUNTY. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4, PRINT ISSN 1222-5304, 248-253.

STUDY OF SEASONAL DYNAMICS IN RESPIRATORY MICROBIAL FLORA IN EXTENSIVELY RAISED GOATS

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Armela-Diana Bordeanu, Florina-Alexandra Krupaci, Timea Kiss, Marina Spînu

An accurate evaluation of the seasonal dynamics of respiratory microbial flora in extensively raised goats represents the first step in early identification of potentially highly pathogenic bacteria in this species. The aim of the study was to monitor the seasonal influence on the bacterial flora of the animals, and therefore a comparative evaluation of changes during winter and spring seasons. The research was carried out on 20 healthy goats, raised under extensive conditions in Transylvania. Nasal discharge samples were cultured on simple media for isolation, and then identified by use of API 20 E and API 20 Staph biochemical tests. During the winter season, out of the total isolated bacterial strains, 26.8% to belonged to E.coli, 14.6% to each Enterobacter aerogenes and Erwinia spp, 12.1% to each Klebsiella pneumoniae and Enterobacter cloacae, 9.7 % to Staphylococcus xylosus and 4.8% to each Chryseomonas luteola and Staphylococcus lentus. During the spring season, changes in both percentages and isolated species soocred. The highest percentages were present in Enterobacter aerogenes (30.5%), followed by each Staphylococcus xylosus and the newly isolated Serratia fonticola (11.2%), sharply decreased E.coli (8.3%) and Erwinia spp. (5.5%), but increased Chryseomonas luteola (8.6%). Rahnella aquatilis (2.7%), Serratia ficaria (5.5%), Serratia liquefaciens (5.5%), Serratia marcescens (2.7%) and Serratia odorifera (8.3%) were present only in the spring season. The bacteria isolated from clinically healty goats could have a highly pathogenic character under critical/stressfull circumstances, which draws the attention to the importance of early identification of pathogens and the acurate sanitary management of the heard

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Bordeanu A. D., Krupaci F. A., Kiss T., Spînu M. 2012, STUDY OF SEASONAL DYNAMICS IN RESPIRATORY MICROBIAL FLORA IN EXTENSIVELY RAISED GOATS. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4, PRINT ISSN 1222-5304, 38-45.

STUDY OF THE MORPHOLOGICAL BASIS IMPLICATED IN INHALATORY ANAESTHESIA AT DOGS: A PERSONAL RESEARCH

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Bors Constantin, Nicolae Cornila, Traian Leau

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Bors C., Cornila N., Leau T. 2012, STUDY OF THE MORPHOLOGICAL BASIS IMPLICATED IN INHALATORY ANAESTHESIA AT DOGS: A PERSONAL RESEARCH. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4, PRINT ISSN 1222-5304, 46-51.

THE CHARACTERISTICS OF LAMENESS IN DAIRY COWS

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Gîscă Eugen Dan

Lameness is considered one of the most important aspects of the animal health besides breeding problems and mastitis. This evaluation is based probably partially on the incidence of this clinical sign and the economical importance of accidental diseases. In another sense lameness could have even a greater importance. Well established control protocols can be implemented regarding both mastitis and breeding problems, but not in the case of bovine lameness problems where such protocols need specification depending on the numerous factors such as raising conditions, forage management, the capacity of farmers to understand that high production is achieved with the price of functional efficiency of the animals. In addition to the multitude of risk factors we have the permanent evolution of nutrition practice and management systems. The latter serve the best interest of man rather than cow comfort. On the farm where the study has been developed cow lameness was around 20%, the conditions that generated lameness were the following: 5% sole ulcer, 5% heel erosion, 4% necrobacilosis, 2% interdigital dermatitis, 3% interdigital phlegmon, 1% claw deformity. The high percentage of lame cows suggest the presence of subclinical laminitis in the herd, and it is advisable to note these conditions in special records, and develop a more extensive investigation, including monitoring the nutrition management.(1) Other painful conditions such as renal colic or abomasum dysplasia are not as frequent as lameness.

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Gîscă E. D. 2012, THE CHARACTERISTICS OF LAMENESS IN DAIRY COWS. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4, PRINT ISSN 1222-5304, 190-197.

THE EFFICACY OF DIFFERENT ACARICIDES AGAINST THE HARD TICK DERMACENTOR MARGINATUS ON INFESTED SHEEP

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Tiberiu Constantin, Iulia Paraschiv, Mariana Ioniţă, Ioan Liviu Mitrea

The aim of the present study was to evaluate the efficacy of four acaricids, from different pharmacologic groups, in control of tick infestation in sheep. Additionally, clinical specific aspects of the infestations were registered. The study was carried out during of March – April 2012, in a southern area of Romania (Colibasi village, Giurgiu County). The therapeutic efficacy of four different acaricides: Deltamethrin (pirethroid), Amitraz (formamidine), Diazinon (organophosphate), and Ivermectine (macrocyclic lactone) on natural tick infestation in sheep was evaluated. The animals (n=40) were divided into four groups (n=10/group) corresponding to the four used drugs. The first three products were used by dipping, in concentration of 0.05% for Delthametrin, 0.05% for Amitraz, and 0.04% for Diazinon. Ivermectine (1%) was subcutaneously injected (0.2 mg/kg). The animals were examined before and after treatment at 3, 5, 7, 14 and 21 days. The number of ticks per animal, the main elective body regions for tick attachment, and the associated lesions, were also registered. A total number of 1054 ticks (938 females, 116 males) were collected from infested sheep, all belonging to the Dermacentor marginatus species. The body areas highly infested were, in order of prevalence: the substernal area (63.6%), sides of the neck (14.2%) and the tail (7.1%). Lesions associated with tick infestation consisted of local irritations and inflammations, nodular dermatitis and micro abscesses. The best acaricidal efficacy was registered for Delthametrin (79.5%, at 3 days and 100% at 5 days p.t.) and Amitraz (90.4%, 96.4% and 100% at 3, 5 and 7 days p.t. respectively); both acaricids preserved full protection at 14 and 21 days after treatment. Ivermectin reached maximum efficacy at 7 days p.t. (92%), afterwards dropping at 79.8% at 21 days p.t..

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Constantin T., Paraschiv I., Ioniţă M., Mitrea I. L. 2012, THE EFFICACY OF DIFFERENT ACARICIDES AGAINST THE HARD TICK DERMACENTOR MARGINATUS ON INFESTED SHEEP. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4, PRINT ISSN 1222-5304, 372-379.

THE IMPORTANCE OF INTRAOPERATIVE RETROBULBAR BLOCK ON ANESTHETIC MANAGEMENT OF ENUCLEATION

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Gina-Teodora Girdan, Iuliana Ionaşcu

One of the major goals of anesthesia is control the pain. For far too many years the veterinary profession has considered analgesia and pain management to be of little interest with practitioners as quoted outdated aphorisms as “ animals feel less pain than humans”, or “pain is beneficial because it limits movement”and “analgesia mask clinical degeneration ”. Now we know that all these are totally false and that pain affects life and rehabilitation of patients in a tremendous way. The aim of this article is to clarify specific conditions that occur in anesthesia following retrobulbar block using as reference parameters : pupillary reflex and position of the eyeball, before and after the block but also the heart rate and non invasive blood pressure.

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Girdan G. T., Ionaşcu I. 2012, THE IMPORTANCE OF INTRAOPERATIVE RETROBULBAR BLOCK ON ANESTHETIC MANAGEMENT OF ENUCLEATION. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4, PRINT ISSN 1222-5304, 184-189.

THE PRESENCE OF MYCOTOXINS (OTA AND ZEA) IN FEED FOR PIGS AND THEIR INFLUENCE ON REPRODUCTION

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4
Written by Catalina POSEA, Alexandru ȘONEA, Alin BÎRTOIU, Monica ROMAN, Mihaela VASILE Anamaria BENCSE, Camelia ION ,

Today FAO estimates that about quater of world cereal crops are contaminated with mycotoxins. Contamination of agricultural products ca occur both befor and after harvest, especially during storage, being conditioned by the humidity and high temperatures. These factors favor the growth of mold and increase the resk of mycotoxins.Species of fungi: Aspergillus, Penicillium and Fusarium can produce and release secondary metabolites in feed type: Ochratoxin A (OTA), Zearalenone (ZEA), impact on reproductive performance. Toxicity of mycotoxin depens on the source and their dose, duration of exposure and composition. Damaging effects of moldy feed management are felt especially in youth and female reproduction subject. If consumption of moldy feed, the effects recorded in breeding disorders manifested by abortions, complications of parturition, uterine involutions delayed, prolonged estrus after calving, followed by infecunditate endometritis and sterility. In this case the percentage of pregnant females is very low, even after the repeated treatements. This paper aims to address the presence of mycotoxins (OTA and ZEA) in feed intended for pigs, with their direct influence on reproduction. To minimize the impact of the presence of mycotoxins in pig feed, control measures are carried out to establish the quality of feed used. This mycotoxin has been evidenced by laboratory tests. The working method used was ELISA. Values obtained from determinations were performed according to the legislation.

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POSEA C., ȘONEA A., BÎRTOIU A., ROMAN M., VASILE M., BENCSE A., ION C. 2012, THE PRESENCE OF MYCOTOXINS (OTA AND ZEA) IN FEED FOR PIGS AND THEIR INFLUENCE ON REPRODUCTION. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 4, PRINT ISSN 1222-5304, 322-329.


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