Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 2
Written by Ioana ENE, Ovidiu ROȘU, Mario CODREANU

This study evaluates an anesthetic protocol for 10 mixed-breed horses (2-22 years old) that have undergone field surgical castration. Premedication was administered intravenously in the left jugular vein with Detomidine (X̅ = 0.02 mg/kg). Induction was achieved with a combination of Ketamine (X̅ = 2.38 mg/kg) and Midazolam (X̅ = 0.038 mg/kg), administered intravenously, in the same syringe. Heart rate (X̅ = 43.46 bpm), respiratory rate (X̅ = 20.65 bpm), capillary refill time (X̅ = 1.26 sec), Oxygen saturation (X̅ = 85.73), and rectal temperature (X̅ = 37.15 °C) were measured from induction until full recovery. We evaluated the time between premedication and induction (T0 - X̅ = 5.5 minutes), from induction until lateral recumbency (T1 - X̅ = 1.4 minutes), surgery duration (T2 - X̅ = 10.5 minutes), time from the surgery until recovery in standing position (T3- X̅ = 22.9 minutes). All animals required assistance until complete recovery and were ataxic while standing/walking for T4- X̅ = 12.3 minutes. The anesthetic protocol provided good analgesia and muscle relaxation. All horses recovered well and no postoperative complications were seen.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 2
Written by Oana POP (COZMA), Carmen Dana ȘANDRU, Diana OLAH, Gheorghiță DUCA, Mariana RUSU, Aurel VASIU, Emoke PALL, Cristian VĂLEANU, Marina SPÎNU

The active principles extracted from plants prove to be useful in preventing or treating various diseases through their influence at molecular level. This potential of plants could reduce the negative effects of existing therapies due to lesser side effects and the results of bioavailability studies are encouraging. Hydro-alcoholic Salvia glutinosa L. extracts were examined for their antioxidant potential, anti-bacterial activity and in vitro immune stimulating effects. The dry aerial part of the plant (herba) was used for these experiments, after minced and solubilized in ethanol and also in aqueous solution. The antioxidant capacity was investigated by free radical scavenging effect over 1,1-diphenyl l-2-picrylhydrazyl (DPPH) radical, the antimicrobial effect by the Kirby Bauer radial diffusion test and the immune stimulating effect by tests on whole blood cultures. The results indicated a stronger antioxidant capacity (RSA% 88.89), antibacterial effect (G - E. coli, P. aeruginosa, x = 12 mm < G+, S. aureus, ATCC/clinical strains, x = 23 mm) and immune stimulation of the ethanol extract compared to the aqueous one. These results indicated the plant as a potential complex source to be implemented in alternative therapy.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 2
Written by Mihai DINICĂ, Andrei CĂLINOIU, Andreea NEACŞU-NIŢU, Cristian MARIN, Ştefania Mariana RAITA

Following numerous pieces of research that highlighted the value of histological techniques applied in assessing the quality and safety of meat food products, a histological examination has become mandatory in many countries. This paper describes in detail the quality characteristics of poultry meat from two different rearing systems. Fragments were taken from the breast of chickens raised in a household system and an intensive industrial system. The fragments taken were processed using the usual histological techniques and stained by the H & E (Hematoxylin & eosin) and Mallory staining methods. After analyzing the permanent histological preparations, numerous differences were observed between the two growth systems. In the household system, the muscle fibers are more developed, which shows that they do a lot of movement, at the same time, the white adipose tissue is less represented. In the industrial system, the muscle fibers show a smaller number of striations, which indicates that they do no movement, a large amount of white adipose tissue being observed. Through the histological evaluation of the chicken muscle tissue from the two different growth systems, a series of differences resulted.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 2
Written by Daniel LESCAI, Bianca BAROIU, Anca CRISTEA, Anca RUSU, Adriana STANCU

The objective is to describe the outcome and complications of two cats treated with a hemi plus rostral part contralateral mandibulectomy (1 ½ mandibulectomy) technique for the management of oral squamous cell carcinoma (SCC) with bone infiltration. Mandibulectomy can be performed in cats but unlike canine patients, they may require additional supportive care. Two cats were presented for progressive mass growing on the mandible. Both had bone invasion and were diagnosed with SCC by biopsy examination. The procedure involved a left/right mandibulectomy and the rostral part of the right/left hemimandible caudal to the lower canine tooth (1 ½ mandibulectomy), at least 1 cm far from the macroscopically visible lesions. Both cats had feeding tubes placed. The surgical outcome for one of the two cats was excellent, surpassing 302 days of survival, the other cat did not regain the ability to eat and the owners opted for euthanasia 35 days after surgery. Both histopathological reports confirmed SCC and clean margins. The hypothesis of the study reported here was that 1 ½ mandibulectomy would be effective for control of superficial subcentimetrical, caudal to the canine tooth oral SCC with bone invasion in cats but could also lead to permanent loss of feeding function and compromised quality of life.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 2
Written by George MOGOȘ, Anca BULGARU, Elena NEGRU, Costin STOICA, Mihai DANEȘ, Doina DANEȘ

The aim of the current study was to develop an auto-vaccine based on two Pasteurella strains (P. multocida and P. spp.), previously isolated from an outbreak of respiratory infection in sheep. Preliminary tests were performed to assess the pathogenicity of the isolates on Balb/C mice. For the vaccine production, the Pasteurella strains were cultured on brain-heart infusion (BHI) medium supplemented with horse serum, were inactivated with formaldehyde and adjuvanted with aluminium hydroxide. In vitro and in vivo tests were performed to determine the sterility, safety and efficacy of the finished product. The results showed that the autovaccine had minimal side effects on both laboratory animals, and the target species; also, the biological product was successful in protecting vaccinated mice against the two pathogenic strains in a challenge test.

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COMPARATIVE LEVELS OF LEAD AND CADMIUM IN SHEEP WOOL AND COW HAIR

Written by Emanuela BADEA, Gheorghe GORAN, Cristina ȚOCA, Cristian MUȘETESCU
Pages 13 to 16

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COMPARATIVE HISTOLOGICAL STUDY BETWEEN FILLET MUSCLE TRADITIONAL PREPARATION AND INDUSTRIAL PREPARATION

Written by Andrei CĂLINOIU, Mihai-Romeo DINICĂ, Laura-Cătălina DUMITRA, Ștefania Mariana RAITA
Pages 17 to 22

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MICROSCOPIC DIFFERENCES BETWEEN MUSCLE TISSUE IN BIRDS RAISED IN HOUSEHOLD VS. INTENSIVE SYSTEM

Written by Mihai DINICĂ, Andrei CĂLINOIU, Andreea NEACŞU-NIŢU, Cristian MARIN, Ştefania Mariana RAITA
Pages 23 to 27

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THE IMPACT OF AGE ON SOME BIOCHEMICAL PARAMETERS IN FELINES WITH CHRONIC KIDNEY DISEASE

Written by Ioana-Nicole REU, Iuliana CODREANU
Pages 28 to 32

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EVOLUTION OF THE REPRODUCTIVE SYSTEM MORPHOLOGY IN ROOSTERS FED ON VITAMIN A AND VITAMIN E SUPPLEMENTED DIETS

Written by Diana ȘIPOTEANU, Mădălina CIOARIC, Ivona ZĂBAVĂ, Emilia CIOBOTARU-PÎRVU, Nicolae DOJANĂ
Pages 33 to 40

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BOVINE HERPES VIRUS QUANTIFICATION BY qPCR IN THE BLOOD OF ASIMPTOMATIC LATE-TERM COWS

Written by Nicolae Tiberiu CONSTANTIN, Florin Petrișor POSASTIUC, Crina Raluca ANDREI, Ovidiu GEICU, Florin IORDACHE, Loredana STANCA, Liviu BÎLTEANU, Andreea Iren ȘERBAN
Pages 41 to 46

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DERMAL MELANOMAS IN A GREY HORSE: CASE STUDY

Written by Alexandra Mihaela CRISTIAN, Niculae TUDOR, Mario CODREANU, Ana DOBRIN, George NICOLAE
Pages 47 to 53

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COMPLEX INVESTIGATIONS IN DIAGNOSIS OF MAREK’S DISEASE IN POULTRY

Written by Georgeta DINESCU, Elvira GAGNIUC, Gabriel TOMESCU
Pages 54 to 61

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FIELD CASTRATION OF TEN STALLIONS: ANESTHESIA AND RECOVERY MONITORING

Written by Ioana ENE, Ovidiu ROȘU, Mario CODREANU
Pages 62 to 65

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COMPARATIVE LEVELS OF ANTIBIOTIC RESISTANCE IN PIGS RAISED UNDER DIFFERENT TECHNOLOGIES

Written by Anca GOCAN (SECARĂ), Carmen Dana ȘANDRU, Diana OLAH, Gheorghiță DUCA, Constantin CERBU, Aurel VASIU, Emoke PALL, Cristian VĂLEANU, Marina SPÎNU
Pages 66 to 71

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USE OF VETSHIELD®/SOFTSHIELD® COLLAGEN CONTACT LENSES IN MELTING CORNEAL ULCERS IN DOGS: 342 CASES (2013-2022)

Written by Iuliana IONASCU
Pages 72 to 82

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THE APPEARANCE OF DIARRHEA IN THE NEONATAL CALF PERIOD - CASE STUDY

Written by Maria Alexandra JALBĂ, Mihai-Alexandru PITRAN, Valentin Alexandru DUȚULESCU, Mario CODREANU, Iuliana CODREANU
Pages 83 to 87

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OUTCOME OF 2 CATS WITH SQUAMOUS CELL CARCINOMA TREATED WITH 1 ½ MANDIBULECTOMY

Written by Daniel LESCAI, Bianca BAROIU, Anca CRISTEA, Anca RUSU, Adriana STANCU
Pages 88 to 96

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PRODUCTION AND EFFICACY EVALUATION OF A PASTEURELLA AUTOVACCINE FOR SHEEP

Written by George MOGOȘ, Anca BULGARU, Elena NEGRU, Costin STOICA, Mihai DANEȘ, Doina DANEȘ
Pages 97 to 102

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IS ANTIOXIDANT CAPACITY CONNECTED TO BIOLOGICAL EFFECTS OF SALVIA GLUTINOSA L.?

Written by Oana POP (COZMA), Carmen Dana ȘANDRU, Diana OLAH, Gheorghiță DUCA, Mariana RUSU, Aurel VASIU, Emoke PALL, Cristian VĂLEANU, Marina SPÎNU
Pages 103 to 108

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THE EFFECT OF GENERAL ANESTHESIA ON UREA AND CREATININE VALUES IN A GROUP OF DOGS

Written by Maria Roxana TURCU, Cătălin MICȘA, Lucian IONIȚĂ, Ruxandra COSTEA
Pages 109 to 112

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THORACIC TRAUMA UPDATES IN FELINE HIGH-RISE SYNDROME. WHAT CHANGED IN 30 YEARS? 50 CASES IN ONE YEAR

Written by Seralp UZUN, Iuliana IONASCU, Florin DUMITRESCU, Dragos-Marian DUMITRASCU, Tiberiu Sebastian IANCU, Radu JERCAU, Catalina GEORGESCU, Dumitru MAGALEAS, Georgeta FILIP, Ada IONESCU
Pages 113 to 122

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SURVEY ON FACTORS AFFECTING HONEY BEES COLONIES IN ROMANIA: PRELIMINARY DATA

Written by Daniela CERBU (BOANFA), Gheorghe DOBRE, Emanuel MITREA, Andreea Cristina PALTIN, Ioan Liviu MITREA, Mariana IONITA
Pages 123 to 129

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COMPARATIVE STUDY OF SOME TRACE ELEMENTS AND MACROMINERALS IN PIG LEG DEPENDING ON THE COOKING METHOD

Written by Gheorghe Valentin GORAN, Emanuela BADEA, Carmen Daniela PETCU, Oana Diana MIHAI, Liliana TUDOREANU
Pages 130 to 134

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COMPARATIVE STUDY ON THE VARIATION OF THE SERUM CORTISOL LEVEL DEPENDING ON THE CATTLE SLAUGHTERING METHOD

Written by Oana Diana MIHAI, Carmen Daniela PETCU, Dana TĂPĂLOAGĂ, Corina PREDESCU, Gheorghe Valentin GORAN, Elena MITRĂNESCU, Emanuela BADEA, Oana Mărgărita GHIMPEȚEANU, Emilia CIOBOTARU-PÎRVU
Pages 135 to 143

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THE EFFECT OF VARIOUS TYPES OF FLOUR AS FILLER MATERIALS ON PHYSICAL, CHEMICAL AND ORGANOLEPTIC CHARACTERISTICS OF SALAMI CULLED LAYING HENS

Written by Sofi Margritje SEMBOR, Hengkie LIWE, Nova Nancy LONTAAN, Merry Diana ROTINSULU
Pages 144 to 154

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THE DEVELOPMENT OF A PRECLINICAL MODEL FOR OSTEOINTEGRATION OF DENTAL IMPLANTS - A PILOT STUDY

Written by Diana-Larisa ANCUȚA, Maria CRIVINEANU, Cristin COMAN
Pages 155 to 161

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COST ANALYSIS OF TISSUE MICROARRAYS FOR CLINICAL DIAGNOSTIC

Written by Alina Elena ȘTEFAN, Daniela GOLOGAN, Sorin MUȘAT, Matthew Okerlund LEAVITT, Raluca STAN, Manuella MILITARU
Pages 162 to 168

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 2
Written by Daniela CERBU (BOANFA), Gheorghe DOBRE, Emanuel MITREA, Andreea Cristina PALTIN, Ioan Liviu MITREA, Mariana IONITA

The longevity of honey bees, although genetically conditioned, is impacted by numerous factors including diseases, parasites, pesticides, predators, but also by environmental and socio-economic factors. Additionally, the decrease in pollen resources reduces the queen’s brood and finally the longevity of the bee colony. Therefore, a survey, based on clinical examinations in apiaries and a questionnaire completed by beekeepers was conducted in 2023 to analyze the major factors that adversely impact the honey bees colonies. For this a total of 50 beekeepers from five counties in Central and South-eastern Romania were enrolled in the study. Among the factors causing honey bee losses, diseases (including varroosis, wax moth, nosemosis, others) and unfavourable climatic conditions, such as longer dryness and cold periods, rains, or strong winds were reported. These findings emphasize on the importance of continuous monitoring, investigations, and specific control measures to be taken in order to preserve the health and activity of honey bee colonies.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 2
Written by Maria Alexandra JALBĂ, Mihai-Alexandru PITRAN, Valentin Alexandru DUȚULESCU, Mario CODREANU, Iuliana CODREANU

Neonatal calf diarrhea syndrome, also known as calf scours or enteritis, is a common condition that affects young calves, particularly those between one and three weeks of age. This syndrome has a multifactorial etiology and has a negative impact on farm economics and welfare. The severity of diarrhea can range from mild to severe, and it can lead to dehydration, electrolyte imbalances, and even death in severe cases. Early recognition and prompt treatment of diarrhea are essential to minimize the negative impact on calf health and productivity. This study was conducted on a private farm at the request of the owner on a 3-week-old female in October 2022. The clinical examination was requested due to changes in the general condition such as apathy, uncontrollable diarrhea, dehydration, colic syndrome, inappetence, high body temperature. This abstract provides an overview of the causes and management of diarrhea in neonates, including prevention strategies and treatment options. Understanding the appearance of diarrhea in the neonatal period is crucial for healthcare providers and caregivers to ensure the optimal health and well-being of newborns.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 2
Written by Diana-Larisa ANCUȚA, Maria CRIVINEANU, Cristin COMAN

Functional tooth replacement and bone regeneration are areas of interest in modern dentistry and dental implant research involves increased attention to osteointegration. The aim of the study was to develop a small, inexpensive and reproducible animal model for testing dental implants. Fifteen male Wistar rats, 20 weeks old, average weight of 400 grams were included in the study. They were subjected to a rigorous bone support preparation protocol so that the maxillary first premolar was extracted from the left half arch. After a period of 30 days, necessary for the bone refilling of the dental alveolus, the radiological examination was performed. Then a surgical intervention was performed to mount the titanium implants of an adapted size. Clinically, the evolution was favorable, with no signs of discomfort or oral infection. At the radiological evaluation, optimal bone regeneration could be observed. necessary to ensure a suitable place for implant mounting. The implantation procedure was laborious due to the limited working area. However, rats are proving to be suitable animal models for implant-related studies or innovative treatments administered under pathological conditions.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 2
Written by Maria Roxana TURCU, Cătălin MICȘA, Lucian IONIȚĂ, Ruxandra COSTEA

All anesthetic drugs act on renal function, the kidney being the primary organ involved in the excretion of the anesthetic drugs. Also, anesthesia influences kidney function, especially through hemodynamic and neuroendocrine changes. Anesthesia and renal function are highly interconnected and can potentially influence each other. Understanding the anesthetic effects on renal function can help to develop safe perioperative care and to optimize the after-surgery outcome. The study will focus on how general anesthesia influences renal parameters, blood urea nitrogen (BUN) and creatinine (CREA). The results of our study show the impact of general anesthesia for both BUN and CREA, in correlation with the medication used for premedication, and the patient's condition.

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