Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 2
Written by George MOGOȘ, Anca BULGARU, Elena NEGRU, Costin STOICA, Mihai DANEȘ, Doina DANEȘ
The aim of the current study was to develop an auto-vaccine based on two Pasteurella strains (P. multocida and P. spp.), previously isolated from an outbreak of respiratory infection in sheep. Preliminary tests were performed to assess the pathogenicity of the isolates on Balb/C mice. For the vaccine production, the Pasteurella strains were cultured on brain-heart infusion (BHI) medium supplemented with horse serum, were inactivated with formaldehyde and adjuvanted with aluminium hydroxide. In vitro and in vivo tests were performed to determine the sterility, safety and efficacy of the finished product. The results showed that the autovaccine had minimal side effects on both laboratory animals, and the target species; also, the biological product was successful in protecting vaccinated mice against the two pathogenic strains in a challenge test.
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