INTEGRATED USE OF CARBOHYDRATES AND PHENOLIC STRUCTURES FOR THE FRACTIONING OF LIGNOCELLULOSIC RESIDUES

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Letiţia Purdoiu, Viorica Chiurciu, Alexandru Nicolae Popescu, Elisaveta Ţuluca, Cristina Dinu-Pîrvu, Maria Ichim, Lucian Ioniţă, Simona Ivana

The paper presents the results of fractioning the biomass towards its bioconversion to ethanol for both – the carbohydrates resulting from hemicelluloses and from the ones of the lignocellulosic complex. Therefore not only methods of chemical hydrolysis were applied, but enzymatic ones too, using products developed in our own laboratories. Also, the possibility of turning into account the phenolic compounds – with high antioxidant potential – was closely looked into, as the phenols are the result of the enzymatic cleavage of the lignocellulosic edifice, rigidly structured. This treatment, unlike the acid hydrolysis, does not cause degradations in the aromatic structures, when submitted to high temperatures. The distillation process, as well as the correction one for the ethanolic solutions that came both from the pretreatment phase and from the celluloso – lythical saccharification phase were performed in the same fashion, using the identical methodology. The results were, therefore, comparable and compared as considering the bioconversion yields and the „INVENTA AG” protocol, applied in Switzerland, through which a quantity of 95% ethylic acid is obtained, that is of 240 liters/tone ligneous dried residue.

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Purdoiu L., Chiurciu V., Nicolae Popescu A., Ţuluca E., Dinu-Pîrvu C., Ichim M., Ioniţă L., Ivana S. 2012, INTEGRATED USE OF CARBOHYDRATES AND PHENOLIC STRUCTURES FOR THE FRACTIONING OF LIGNOCELLULOSIC RESIDUES. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3, PRINT ISSN 1222-5304, 372-380.

MONITORING OF HEAMATOLOGICAL INDICES IN A SAMPLE GROUP OF CATS SUBJECTED TO SERIAL BLOOD COLLECTIONS FOR BIOEQUIVALENCE TESTING

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Laurenț Ognean, Cristina Cernea, Alexandra Arion, B. Benedek, M. Imre, Moldovan Maria Meda, Sebastian Trîncă, Ildikó Barabási

The bioequivalence evaluation of a drug is based on the bioavailability of its active molecule. Serial blood sampling might be limited in cats, due to their reduced blood volume and other morphophysiological characteristics that will be analyzed in this study. The bioequivalence testing of an antihelmintic product was conducted on 37 common breed cats, between 2 to 4 kg, fed with dry and canned food. The testing protocol consisted of two phases, 14 days apart. Eleven blood samples were collected at increasing time periods (0 - 24h). The total blood volume collected in each stage was evaluated based on the volemia, estimated at 7.5% from the total body weight, representing a maximum of 13 ml/kg and 2.2 ml for each sample. Along with the serial blood sampling, at the start and ending of the two phases, haematological evaluations (on EDTA) and biochemical profiles (on Li-Heparin) were performed. Addtionally, morphological assessments were carried out on panoptic stained smears. No major alterations of the physiological parameters were recorded, except for a small decline in the erythrocitary parameters, associated with oscillations of the total white blood cell count and a tendency to monocytosis. The results of this study reveal the necessity to associate physiological parameters of the tested animals with the requirements of drugs bioequivalence testing protocols, in order to respect ethical and good practice standards while collecting multiple blood samples.

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Ognean L., Cernea C., Arion A., Benedek B., Imre M., Moldovan M. M., Trîncă S., Barabási I. 2012, MONITORING OF HEAMATOLOGICAL INDICES IN A SAMPLE GROUP OF CATS SUBJECTED TO SERIAL BLOOD COLLECTIONS FOR BIOEQUIVALENCE TESTING. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3, PRINT ISSN 1222-5304, 180-189.

MONITORING THE RISKS OF SOME PHYSIOLOGIC FACTORS OF LACTATION IN PIC SOWS ON THE HEALTH OF SUCKLING PIGLETS

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Adrian VLASIU, Laurenţiu OGNEAN, Marius Gh. BEREŞ., Horia SARANDAN, Cristina CERNEA, Sebastian TRÎNCĂ, Rodica SOCACIU

The influence of the major physiological factors (age, weight at first farrow and some genotypic, phenotypic and environmental characteristics) on prolificacy and sow lactation capacity as well as the growth and health indices in piglets was monitored on a PIC industrial multiplication farm with a breeding effective of 200 sows and a total of 255 farrows, in 2011. Evaluations were based primarily on quantification of biometric parameters of the farrows. Am overview of the data regarding the influence of age (farrow rank) on milk production in sows revealed a maximum production level in the 2nd or 3rd lactation in correlation with increased prolificacy (11.9 or 11.7) and lactation capacity (65 and 64) respectively with reduction of mortality in piglets (3.6% and 3.7%). The evolution of these indices revealed that sows can be effectively exploited in the first 5 lactations, after which advancing age significantly limits milk production. Achieving the first fertile optimal age of first farrowing (233-238 or 348-353 days) also contribute to ensuring milk production. The lactation curve was characterized by an upward phase in the first 7-10 days, a plateau of about 10 days and a downward phase, consisting of slow decrease milk production followed by its marked reduction around weaning. In last year the total recorded farm losses (10.2%) were represented largely by mortality in suckling piglets (45%).

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VLASIU A., OGNEAN L., Gh. BEREŞ M., SARANDAN H., CERNEA C., TRÎNCĂ S., SOCACIU R. 2012, MONITORING THE RISKS OF SOME PHYSIOLOGIC FACTORS OF LACTATION IN PIC SOWS ON THE HEALTH OF SUCKLING PIGLETS. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3, PRINT ISSN 1222-5304, 260-269.

MORPHOFUNCTIONAL CORRELATIONS OF THE FOREARM MUSCLE AT SHEEP AND DOG

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Ştefănescu, S., G. Predoi, C. Belu, B. Georgescu, I. Dumitrescu, Carmen Biţoiu, Anca Şeicaru

At both ovines and canines, the carpal, metacarpophalangeal and interphalangeal joints are predominantly organized in order for flexion and extension to be possible.cSince these movements are made in the same way for all joints mentioned, extensor muscles are grouped on the back and flexor muscles are on the palmar. At dogs, supinator muscles are framed in terms of topography, in extensor, dorsal muscle group and the pronator muscle in flexor, palmar muscle group.

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Ştefănescu, Predoi S. G., Belu C., Georgescu B., Dumitrescu I., Biţoiu C., Şeicaru A. 2012, MORPHOFUNCTIONAL CORRELATIONS OF THE FOREARM MUSCLE AT SHEEP AND DOG. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3, PRINT ISSN 1222-5304, 222-227.

MULTILINEAR CAPACITY ASSESSMENT OF MOUSE MESENCHYMAL STEM CELLS

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Ilea Ioana Cristina, Pall Emoke, Ciupe Simona, Cenariu M., I.S.Groza

Mesenchymal stem cells (MSCs) are defined as bone marrow derived cells which have the capacity to differentiate into the three classical mesodermal lineages (adypocites, osteoblasts and chondrocytes). Different studies revealed the ability of bone marrow derived MSCs to differentiate into ectodermic lineages including neurons. The aim of our study was to evaluate the multipotency of mouse bone marrow derived MSCs by phenotypic characterization during neuronal induction. Mouse MSCs were isolated from bone marrow by flushing the femurs with α MEM (Gibco) medium supplemented with 1% penicillin-streptomycin (Gibco). Isolated cells were cultured in a propagation medium containing DMEM-F12 medium supplemented with 20% FCS (Gibco), 1% penicillin-streptomycin (Gibco), 5% horse serum (Sigma) and 10μg/5ml MycoZap (Mycoplasma Elimination Reagent, Lonza). For neural induction, cells were cultured in Neurobasal medium supplemented with 0.1mM β-mercaptoethanol and 1% glutamax for 2 weeks. For phenotypic characterization, were evaluated the expression of S-100 protein and neuron specific enolase (NSE) during differentiation. Our results confirmed the multipotency of isolated cells by neuronal differentiation. At 3 days after neurogenic induction, cells morphology changed, appearing star-shaped cells and at day 4 were present specific neuritic networks. At 2 weeks after induction, the immunostaining showed the presence of S-100+ cells, confirming the glial differentiation, as well as NSE+ cells, an indicator of neuronal differentiation.

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Ilea I. C., Emoke P., Ciupe S., Cenariu M., Groza I.S. 2012, MULTILINEAR CAPACITY ASSESSMENT OF MOUSE MESENCHYMAL STEM CELLS. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3, PRINT ISSN 1222-5304, 146-153.

NONCOMPLIANCES WHICH LEADS TO AN INEFFICIENT PEST CONTROL IN MEAT PROCESSING PLANTS

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Gabriela Rusen

The aim of this study is to avoid the mistakes which lead to an inefficient pest control in meat processing plants. Pest control programme are an essential part of maintenance and sanitation. Pest poses a major threat to the safety of the food products. Unfortunately, in many cases I found that the pest control programmes were not correctly created and applied, so I met a lot of mistakes which should be avoided for an efficient prevention. Pests (insects, rodents, birds, dogs, cats) entering or infesting food plants are a significant potential source of microbiological, physical and chemical hazards (use of pesticides). Pests are carries for many microorganisms, pests are source of foreign bodies (insects themselves, hair, larvae etc.) and insects can transfer contamination from dirty areas to clean areas. The target of pest control is to prevent the pest ingress in the plant and not to combat them inside. Prevention is critical in pest control.

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Rusen G. 2012, NONCOMPLIANCES WHICH LEADS TO AN INEFFICIENT PEST CONTROL IN MEAT PROCESSING PLANTS. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3, PRINT ISSN 1222-5304, 381-384.

NUTRITIONAL AND METABOLIC PARAMETERS IN LAYING HENS FED WITH DIFFERENT LEVELS OF CALCIUM, PHOSPHORUS AND PHYTASES

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Roşu M., Sărăndan H., Violeta Turcuș, Sarandan M.

The experiment was made on a Lohmann Brown classic hybrid from 32 to 42 weeks of age. There were used feeds containing various levels of Ca, P or phytase: NC1 and NC2 were commercial recipes, NC3 was supplemented with Phyzyme (500 TFU/kg), NC4 was supplemented with monosodium phosphate and NC5 with dicalcium phosphate according to nutrient requirements (NRC 1998). There was tested the possibility to use the phytase included at a level of 0.12%nPP to make available enough phytic phosphorous to satisfy the birds requirements. (0.25% nPP). When included at a level of 500 FTU/kg feed, Phyzyme hydrolyzed 75% of the phytic phosphorous contributing to a 0.30% aP in the feed. The biological value of the phosphorous in NC3 was of 91.19% and it did not influence either the eggs production or the eggs’ quality.

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Roşu M., Sărăndan H., Turcuș V., Sarandan M. 2012, NUTRITIONAL AND METABOLIC PARAMETERS IN LAYING HENS FED WITH DIFFERENT LEVELS OF CALCIUM, PHOSPHORUS AND PHYTASES. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3, PRINT ISSN 1222-5304, 158-167.

OCCURRENCE OF CAMPYLOBACTER SPP. IN ROMANIAN BROILER CHICKEN PRODUCTION SECTOR

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Laurenţiu Tudor, Anca-M. Galiş, Manuella Militaru1), Elena Mitrănescu

Due to the well-known potential of Campylobacter spp. to determine illness in humans, its detection and occurrence, especially in poultry meat (due to the frequent contamination of this food product) are considered highly important for the consumer’s health point of view. In order to determine the occurrence of Campylobacter spp. in the Romanian broiler chicken production sector, a number of five units were selected and samples were collected as it follows: a total number of 600 samples consisting of chicken skin neck, 600 samples of intact intestines and 1200 samples of fresh chicken carcasses. The results were different from one unit to another, but overall, 54.7 % of the caecal material samples, 48.5 % of the neck skin samples and 31,5 % of the carcass samples were contaminated with Campylobacter spp., with an overall percentage of positive samples reaching 60,2 %. This high occurrence opens the opportunity for future research in order to determine the causes leading to contamination, while also identifying the species of this genus, for a better understanding of this mechanism through which this foodborne pathogen contaminates broiler meat.

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Tudor L., Galiş A. M., Militaru M., Mitrănescu E. 2012, OCCURRENCE OF CAMPYLOBACTER SPP. IN ROMANIAN BROILER CHICKEN PRODUCTION SECTOR. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3, PRINT ISSN 1222-5304, 398-403.

OPERATION AND HYGIENE OF FOOD ESTABLISHMENTS

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Adrian Vasile, Constantin Lupescu, Rareş Popa

Audit is a systematic and independent examination to determine whether activities and results comply with planned programs and whether these programs are implemented effectively and are suitable to achieve objectives. Food business operators shall have in place systems and procedures to identify other businesses to which their products have been supplied. Food business operators must identify any person from whom they have been supplied with a food, a pet food to food or any substance intended or expected to be incorporated into a food product. Food business operators shall cooperate with the competent authorities on action taken to avoid or reduce risks posed by a food which they supply or have supplied.

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Vasile A., Lupescu C., Popa R. 2012, OPERATION AND HYGIENE OF FOOD ESTABLISHMENTS. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3, PRINT ISSN 1222-5304, 404-407.

PLASMA CORTISOL LEVEL AND MAIN METABOLISM EVOLUTION IN PREGNANT EWE

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by N. Dojană, Iuliana Codreanu, Costin Budică

The purpose of this research was to determine the role of glucocorticoid hormones in enhancing metabolic processes characteristic of gestation in ewe. In this respect a group of pregnant ewes was monitored in terms of evolution of plasma cortisol and metabolism during a period of about 14 weeks from the time of pregnancy diagnosis. Parallel determinations were made in a control group consisting of non pregnant ewes. It was determined the evolution of plasma cortisol, plasma glucose, triglycerides, cholesterol, proteins, urea, uric acid, fibrinogen, creatinin, amylase, calcium, phosphorus and magnesium. Results relived progressively increasing of plasma cortisol concentrations in pregnant ewes compared with control. In parallel it was found different evolution of determined blood parameters by comparing with control, meaning different metabolic evolution of the studied parameters in pregnant vs. control ewes. The metabolic evolution of the blood determined parameters in pregnant ewe correlated to an increased secretion of cortisol.

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Dojană N., Codreanu I., Budică C. 2012, PLASMA CORTISOL LEVEL AND MAIN METABOLISM EVOLUTION IN PREGNANT EWE. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3, PRINT ISSN 1222-5304, 126-133.


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