Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXXI, Issue 1
Written by Claudia DIRLA, Mihai TURCITU, Maria Rodica GURĂU, Fănel OȚELEA, Marius Andrei MĂNESCU, Doina DANEȘ
ASF is a fatal haemorrhagic disease affecting pigs, posing a significant threat to global agriculture as it rapidly spreads across the UE, Asia, and Oceania. Due to the lack of a vaccine or treatment, each outbreak requires pursuit of stringent biosecurity measures and trade restrictions. The disease’s emergence is causing significant economic losses due to the stamping out of pig holdings in the affected areas and the pursuit of disease control measures, including trade restrictions to avoid disease spread. Since the onset of the ASF, Romania has been severely affected economically and socially, suffering losses in commercial and backyard farms, unemployment among workers in the pig breeding and meat processing industries, and significant expenditure on disease control and surveillance. ASF remains a major threat with no available vaccine and challenging eradication. Rapid, sensitive diagnostics are crucial. The proposed workflow offers multiple advantages, including the ability to verify extraction, amplification efficiency for each sample through amplification of two internal control systems, ensuring accurate pathogen detection regardless of nucleic acid type presence, and confirming sample cellularity to validate result accuracy and prevent false negatives due to inadequate collection, transport, or storage.
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