Scientific Works Series C. Veterinary Medicine

PRINT ISSN 2065-1295, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 3
Written by Manuela PASCAL, Ruxandra COSTEA, Alin Ion BÎRTOIU

For the freezing of canine semen, slow to fast cooling rates have been used. Today, there are many ways of achieving this by using manual protocols, automatic protocols or ultrafreezers. Straws were divided in two groups: one batch (10 ejaculates) was automatically frozen, while the other batch was frozen manually. Motility (computer assisted sperm analyzer), morphology and acrosome status (Spermac® stain) were evaluated for fresh and frozen-thawed semen. The manual freezing protocol provided higher total (23.85%) and progressive motility values (20.41%) compared to the automatic protocol (13.26 % total motility, progressive motility 9.90%). The acrosome status was strongly influenced by the cryopreservation process, but there were n significant differences between the two ptotocols.When using the CaniPro Freeze® extender, a slower cooling rate (the manual protocol) gave better results then a fast one (the automatic protocol)

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 3
Written by Anca Sofiana SURPAT, Diana BREZOVAN, Jelena SAVICI, Corina PASCU, Janos DEGI, Ovidiu MEDERLE, Viorel HERMAN

To compare different histopathological methods for diagnosis of Lawsonia intracellularis infection in pigs were taken in study 25 samples of ileum with specific lesions of intestinal adenomatosis. In order to perform slides were used Kinyoun, Green-Methyl-Pironine, Masson-Fontana, Schmitz, Diff-Quick methods and immunohistochemistry. The results showed that Green-Methyl-Pironine method has no value for diagnosis of porcine proliferative enteropathy, while Kinyoun coloration is capable to identify the bacteria only in 28% of samples. The argentic impregnation and Diff-Quick are able to highlight the aetiological agent in 44%, respectively 40% of the studied samples, so this methods have enlarge value of diagnosis. Immunohistochemistry demonstrated a high sensitivity and specificity and it was capable to emphasize the causative agent of intestinal adenomatosis in all 25 studied samples with proliferative ileitis.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 3
Written by Ruxandra COSTEA, Manuela PASCAL, Alin Ion BIRTOIU, Alexandru Ilie DIACONESCU, Monica Elena BURAC

A complete and correct individualized management in the peri-operative period for urogenital surgery is the key for good results. This study presents the peri-operative anesthesia and analgesia protocols, along with all the support addressed for individual patient needs, used in the Clinic of Obstetrics and Gynecology at the Faculty of the Veterinary Medicine used for a number of 71 cases. A good support of circulatory system, individualized fluid therapy, metabolic and nutritional support, intra-operative support, postoperative support, pain management and infection control were applied for all the patients and recovery time was assessed. Delayed recovery from anesthesia was commonly encountered due to hypothermia and metabolic disorders, in geriatric cases.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 3
Written by Roxana DASCALU, Marius SABAU, Adelina PROTEASA, Larisa SCHUSZLER, Aurel SALA, Maria SERB, Cornel IGNA

The aim of this study was to determine the rate of healing of bone tissue correlated with the type of treatment applied at 34 birds, both domestic and wild, presented in the Surgery Clinic between 2005 and 2013, suspected of traumatic orthopedic conditions. In order to remedy these orthopedic disorders it was used either singular fixation methods (bandage / splint, intramedulary nail, external fixator, cerclage) or mixed systems (intramedulary nail + splint / bandage; intramedulary nail + cerclage, external fixator + intramedulary nail). In most cases, we combined methods to counteract the destabilizing forces acting on the fracture. Recent tibiotars, radius and ulna fractures which allowed the application of the biological fixation, involving a closed reduction of bone fragments and a minimally invasive surgical approach, have led to bone healing in a greater proportion. Death of convalescent wild birds was the most common cause for fracture healing failure.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 3
Written by Marius Catalin BUZATU, Mariana IONITA, Ioan Liviu MITREA

A coprological study was performed in working horses (n=148) from 13 villages in northeastern and southeastern Romania. The aim of this research was to obtain current data on the prevalence of intestinal parasites in working horses and, additionally, to establish strongyle EPG profiles for the horses based on the strongyle eggs per gram of feces (EPG) counts. For this, fresh fecal samples, collected over a 5-months (June-October) period in 2013, were analyzed qualitatively for presence of intestinal parasites using sodium chloride flotation technique, and quantitatively, for strongyle EPG using a modified McMaster egg counting technique. Fecal samples of 104 horses (70.3%) were positive for parasite eggs, with an overall prevalence as follows: 70.3% for strongyles, 12.2% for Parascaris equorum, 4.1% for Strongyloides westeri, and 2.7% for Anoplocephalidae. The highest intensity rate belonged to strongyles, with the EPG counts varying from 25 to 2775. Of them, 58.6% had the EPG count <250, 23.1% between 250-1000, while for 10.6% of the positive animals the EPG counts ranged between 1000–2000, and for 7.7% was bigger than 2000. The average (%) of EPG-positive animals by age group was: <1year (5.8%), 1-5 (20.2%), 6–10 (29.8%), 11–15 (32.6%), and >16 years (11.5%). This research showed the value of strongyle EPG profiling for the working horses, important base for further studies in designing and monitoring sustainable control program of equine parasites.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 3
Written by Mircea NICOLAE, Catalin DRAGOMIR, Smaranda POP

In our experience, with a duration of 12 weeks, were used 48 lambs, Merino breed, after weaning, the mean weight of 14 kg. The lambs were divided into 4 groups, each fed a proper diet with different ratios between the alfalfa hay and compound feed as follows: group 1 (80/20), group 2 (60/40), group 3 (40/60) and group 4 (20/80). Complete rations compacted were used, size of 2.5*1.5 cm, clear consisting of alfalfa hay (chopped before) and compound feed. The rations, administrated ad libitum, were izo-nitrogenous (179 g CP/kg DM) and different energy values. Total feed intake (alfalfa hay + compound feed) decreased almost linearly with increasing proportion of concentrates in the rations, being in the order of 4 groups: 1242, 1205, 1155 and 928 g DM/day, between the first and the last value being a difference of 34%, the difference being assessed as significant for P<0.01. Average daily gains evolve quadratic, the highest recorded to the intermediate groups, and lowest at extreme groups. Therefore, the average weight gains were the highest recorded on groups G 60/40 and G 40/60, 250, or 267 g/day, and the small on G 80/20 and G 20/80, 222, or 227 g/day (mean difference between intermediate and extreme groups were 15%). The best yields are obtained at slaughter (dressing) of the groups G 60/40 and G 40/60, 57.2% and 57.8%. Share empty digestive tract and digestive content diminishes with increasing participation of alfalfa hay in rations. A significant increase in live weight of subcutaneous fat was observed, of from 3 to 4.1%, as a result of increasing quantities of concentrate consumption

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 3
Written by EVOLUTION OF HORMONAL CONTROL OF CALCIUM AND PHOSPHORUS METABOLISM IN HENS ACCORDING TO AGE AND EGG PRODUCTION

The aim of this work was to determine the relationship between the calcium and phosphorus metabolism and the levels of the main hormones involved in their blood regulation in laying hen. Two breeds of hens were used in this work, 22 weeks aged each one: White Cornish (CRN), as a breed of low egg production and White Leghorn (LGH), as a breed of higher egg production. The hens were raised in industrial system and they were fed according to the technologic diets. The hens were monitored from 22 to 44 weeks of age for the evolution of the parathormone, vitamin D, calcium and phosphorus levels in the blood plasma. Blood glucose concentration, total lipid, total proteins, albumins, globulins, creatinine, and uric acid were also monitored. Analysis of the hormone evolution relieves a peak of the PTH level in LGH hens, around 30 weeks of age (amounted to 392 pg/mL vs. 198 pg/mL in CRN hens). This peak of PTH is behind the laying peak and it is significantly higher in LGH hens vs. CRN hens. It was remarked that the LGH hens reached the peak of the PTH one week sooner vs. CRN hens. Regarding vitamin D, its plasma level presented a relatively constant evolution in CRN, while in LGH it presented an increase around 30 – 32 weeks-of-age up to 142 pg/mL, then it decreased slowly. Accordingly, in LGH hens, the level of calcium (in mg/dL) raised from 9.9 at the beginning of the laying cycle to 34.4 in the peak of the laying, decreasing then, to 18.0 toward the end of the monitoring period. In CRN hens, at the same age, the values of the plasmatic calcium were: 6.2, 12.9 and 18.0, respectively. The calcium/phosphorus ratio presented an ascendant evolution in both, LGH and CRN breeds, indicating an increasing of the free calcium content of the blood plasma. Plasma albumins ranged between 16.0 and 20.0 mg / mL in the LGH hens and between 19.8 and 22.8 mg / mL in the CRN hens. Uric acid plasma levels have evolved relatively parallel to the laying percentage, showing an intensified protein catabolism, according to laying percentage, in LGH hens. Total lipids followed an ascending evolution up to the peak of the laying, and then they decreased slowly in both low and high production breeds.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 3
Written by Aurel MUSTE, Florin BETEG, Marius MUSTE, Ionel PAPUC, Teodor STROE, Loredana HODIS, Gelu ZEGREAN, Aurel DAMIAN

This study describes the possibility of restoring blood flow by a bypass, in a segment which is disrupted or blocked. Our study presents such a possibility and was performed on five pigs weighing 50 kg who underwent aortal bypass at the infrarenal segment and monitored for 30 days postoperatively. The section has been replaced by a length of 5 cm by arteriotomy and closed by suture. The bypass prosthesis used was Dacron and has been properly placed between the two ends and fixed by continuous suture. Anastomosis was performed end-to-side at both ends, between the ends a 5 cm part of aorta was replaced with the prosthesis. Pigs were monitored hemodynamic in terms of physiological constants and general status, postsurgical, received adequate treatment. Aortic bypass has been shown to be effective without postoperative complications noted.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 3
Written by Ramona CLEP

In order to control paratyphoid infections having a high zoonotic risk and involving significant economic damage, a National Control Programme for mobile Salmonella infections in breeding hens, including relevant serovars represented by S. enteritidis, S. infantis, S. hadar, S. typhimurium and S. virchow, has been implemented in Romania. During 2009-2012, an epidemiological study was conducted based on primary data collected from breeding hen holdings. Samples represented by faeces were taken at a frequency established by the Community legislation while the programme aims at obtaining a prevalence of 1% or less of the flocks with 95% confidence limit for breeding hens. Following the study, 149 strains belonging to the species Salmonella enterica subsp. enterica were isolated. The results obtained show that serotyped mobile Salmonella strains classified serologically into 16 serovars circulated within the breeding hen holdings. 19% of the isolated serovars belonged to S. enteritidis, 5% belonged to S. ifantis, 2.6% belonged to S. typhimurium while the incidence of other serovars was much lower.

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Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 3
Written by Madalina BELOUS

The aim of the research was to investigate the consumer awareness regarding Functional Foods, for a new approach regarding producers in Food Industry. Lately, the consumers are becoming more aware regarding a healthy nutrition, food quality, or food components that can bring a benefit for general health, either general wellbeing or either improving health for some particular diseases. On a particular note, it is generally recognized that calcium and probiotics (milk or dairy products) are interconnected with bone health or digestive health, or the fish rich in omega - 3 fatty acids will reduce risk of heart disease. The study was based on an exploratory research based on other literature and geographical data. The amount of scientific date presented in literature, offer substantial ideas regarding consumer awareness and certain foods and health, with new opportunities for food industry. The study could be a tool to investigate the potential opportunity of functional foods for manufactures in food industry. The study was an exploratory one based on international literature with no research based on Romanian market. Thus, local market is in accordance with European trends, markets and regulatory. The study is trying to create an overview regarding a potential production opportunity for food industry. This will require the collaboration of regulatory authorities, manufactures for food quality and food safety.

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