Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by STUDIES ON HISTOLOGICAL STRUCTURES OF THE ABDOMEN ON ADULT WORKER BEES (APIS MELLIFERA CARPATHICA)
In an initial phase, the studies aimed possibilities of including chitinous anatomical segments by using different fixing solutions in order to produce chitin lysis to facilitate cutting them. Various fixing solutions have been used (glacial acetic acid, Carnoy fixative, trichloroacetic acid, picric acid, acetone, absolute alcohol, Bouin's fixative, etc.) with variable terms in determining the degree of chitin lysis, which proved to be inefficient, the parts included being improper to sectioning (cutting friability and lack of integrity in tissue resulting from staining). In a later stage, they proceeded to a careful dissection, under the microscope, of the anatomical organs of the abdominal cavity in order to dissociate the anatomical segments present at this level to prepare it for inclusion. Processing of samples was also performed under the microscope until their inclusion in paraffin. Detailed histological examination of tissue and cellular structures, captures the structural aspects at a time. The serial sections revealed the histological structure of several organs located in the abdominal cavity. This positioning of organs in sections led to their identification, facilitating their microscopic interpretation.
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abdominal cavity
