PRINT ISSN 1222-5304, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

VIABILITY ASSESSMENT OF BOAR SPERMATOZOA USING FLOW CYTOMETRY

Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 4
Written by Laura PARLAPAN, Simona CIUPE, EmÖke PALL, Mihai CENARIU, Iulia Maria BALACI, Sandu Nicolae PÎNDARU, Ioan Stefan GROZA

During the last decades a technique based on membrane permeability to fluorescent dyes has been developed in order to determine these parameters. The aim of this study was to determine the fresh boar sperm viability by flow cytometry using a triple stain protocol. Fresh boar semen samples were analyzed with FACSCanto II (BD Biosciences) system on the basis of Hoechst 33348, peanut agglutinin-fluorescein isothiocyanate (FITC-PNA) and propidium iodide (PI) staining. Flow cytometric provide an accurate and precise technique which allowed detection of four categories of sperm: live sperm with intact acrosomal and plasma membranes, live sperm with acrosome lesions and intact plasma membrane, dying sperm with damaged plasma membrane and intact acrosome, as well dead cells with damaged plasma membrane and acrosome lesions.

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