DETECTION OF LISTERIA MONOCYTOGENES IN FOOD PRODUCTS USING A POLYMERASE CHAIN REACTION-BASED METHOD IN COMBINATION WITH A STANDARD REFERENCE ENRICHMENT STEP

Published in Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3
Written by Laurenţiu Tudor, Anca-M. Galiş, Elena Mitrănescu, Aneta L. Tudor

Listeria monocytogenes remains one of the many foodborne pathogens that require continuous monitoring. The detection and tracing methods are quickly developed for a better improvement and a quicker identification of this foodborne pathogen that may be present in food samples. A total number of samples consisting of different types of food products were collected from local markets in Bucharest, subjected to enrichment steps (according to EN ISO 11290-1 and ISO 11560) and further on to a PCR analysis. This resulted in eight samples with positive results, among them goat cheese, smoked fish, sausages and raw chicken meat. The identification was possible and quick, no matter the bacterial load of the samples, due to the existence of a virulence gene pertaining to the internalin gene family: InlB, which is specific to L. monocytogenes. This method ensures a high sensitivity and specificity in a very short period of time, reducing the work time for this analysis with five days concerning the negative results and seven days for the positive ones.

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Tudor L., Galiş A. M., Mitrănescu E, Aneta L. T. 2012, DETECTION OF LISTERIA MONOCYTOGENES IN FOOD PRODUCTS USING A POLYMERASE CHAIN REACTION-BASED METHOD IN COMBINATION WITH A STANDARD REFERENCE ENRICHMENT STEP. Scientific Works. C Series. Veterinary Medicine, Vol. LVIII ISSUE 3, PRINT ISSN 1222-5304, 392-397.


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