Scientific Works Series C. Veterinary Medicine

PRINT ISSN 1222-5304, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

CORELLATION BETWEEN THE REAL TIME PCR METHOD USED IN CANINE PARVOVIRUS DIAGNOSTIC AND CLINICAL MANIFESTATION

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVII, Issue 1
Written by Cristian IONICĂ, Maria Rodica GURĂU, Dragoș COBZARIU, Georgeta ȘTEFAN, Dana Mihaela CREȚU, Doina DANEȘ

Canine parvovirus infection is one of the most common diseases of puppies. Dogs are affected by two viruses of the Parvoviridae family: CPV-1 (Bocavirus genus), thought to have minimal pathogenic potential, but having being associated with different disorder in all age category dogs, and CPV-2 (Parvovirus genus), known as Canine Parvovirus, the true parvovirus. Despite the extensive use of vaccination, the prevalence of infection registers an oscillating dynamic, the virus is evolving and the requirement for the confirmation diagnosis is being continuous. The aim of the research was to identify the possibly correlations of the clinical manifestations with the results of CPV-2 detection, using the real time PCR. Fourteen dogs with clinical manifestations associated with the suspicion of canine parvovirus, has been tested on feces samples by real time PCR: 13 were positive (92.85 %) to the real-time PCR test. The registered positiveness has been associated with different Ct values ranging from 6.82 to 35. One sample (7.14%) was negative. In this study, despite the variation range of Ct's, the clinical pattern registered did not directly relate with the virus amount, rather with the age.

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