Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXVI, Issue 1
Written by Emoke PALL, Mihai CENARIU, Simona CIUPE, Ovidiu GRAD, Ioan Stefan GROZA
Platelet rich plasma (PRP) in combination with different type of biomaterials can promote tissue regeneration. Our study aimed to evaluate the adhesion, proliferation and chondrogenic differentiation potential of canine mesenchymal stem cells on functionalised collagen-based biomaterial. Characterized gingival mesenchymal stem cells (1x105) were seeded on PRP functionalized synthetic biomaterial and were cultured in DMEM/F12 culture medium supplemented with 10% fetal calf serum and 1% antibiotics-antimycotics. After 24, 72 h and 5 days cellular adhesion, proliferation was assessed using CCK8 assay. The absorbance was recorded using a a BioTek Synergy 2 microplate reader set at 450 nm. The bioactive potential of PRP functionalized biomaterial was evaluated after 21 days. Proteoglycans and sulfated glycosaminoglycan content were assessed. Statistical analysis was performed using GraphPad Prism 5.0 software and statistical significance was considered when p < 0.05. Our results indicated the potential of PRP on cells attachment, proliferation and due to the release of growth factors promote the cells differentiation.
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