Scientific Works Series C. Veterinary Medicine

PRINT ISSN 1222-5304, ISSN-L: 2065-1295, ISSN CD: 2343-9394,ISSN ONLINE 2067-3663
 

EVALUATION OF ALTERNATIVE ROUTES OF INTRAVENOUS ADMINISTRATION TO THE METHOD OF ADMINISTRATION SUBSTANCES BY PENILE VEIN IN THE IN VIVO ANTIGENICITY TEST IN GUINEA PIGS

Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXV, Issue 1
Written by Cristin COMAN, Teodoru SOARE, Diana ISTRATE, Andrei STOIAN, Diana ANCUȚA Jasmina MANOLESCU

Guinea pigs are rodents still used for scientific purposes in experimental models for tuberculosis, diphtheria, vitamin C deficiency, etc. One of the tests to which the guinea pig is widely used is the in vivo test of antigenicity. In this test, the substances are inoculated intravenously onto a previously sensitized guinea pig and analyzed whether the products tested cause anaphylactic shock. Intravenous inoculation is done, according to procedures, 2 times at 7 days in the intravenous penile vein, therefore only males are used. The purpose of the study was to evaluate intravenous inoculation methodology and welfare of guinea pigs by assessing the reaction produced after 3-way intravenous injection, penile vein, auricular vein and safenous vein, in the antigenicity test. The article describes the intravenous inoculation methodology for each route, the clinical health status assessment and the histological evaluation of the inoculated veins. The results obtained show that the safenous vein represents a safe way of intravenous inoculation, both as a technique and as side effects and histopathological lesions, and can be an alternative to the current method.

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