Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIV, Issue 1
Written by Mirela TRIPON, Emoke PALL, I. MORAR, M. CENARIU, C. CRECAN, Denisa LUNGU, Lavinia SÎNTEONEAN, V. ILAȘ, A. HENEGARIU, Andreea RUSU, I. Ș. GROZA
Collection of spermatozoa from the cauda epididymis may be the last chance to preserve genetic material from valuable stallions in case of sudden death or emergency castration. In the present study we compared the characteristics of extended refrigerated stallion epidydimal spermatozoa at 24 and 48 hours after castration. Spermatozoa from 12 epididymides were recovered at 24 hours after the routine orhiectomy of 6 healthy stallions using the retrograde flush method and refrigerated for 24 hours. For refrigeration we used 2 commercial extenders - an egg yolk based extender (Triladyl®, Minitube) or a milk based extender (Gent®, Minitube). Concentration and motility parameters were assesed for each sample after collection and 24 hours later using computer assisted sperm anlaysis (SCA®, CASA). Viability was assessed using the eosin staining technique. Total motility, velocity, viability and percentage of progressive spermatozoa were similar among the two groups at 24-h post castration. However, at 48 hours the percentage of progressive spermatozoa was significantly higher in milk based extender. Therefore we concluded that stallion epididymal spermatozoa extended in milk protein based extenders can be successfully cryopreserved at 48 hours after routine orhiectomy.
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