Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIII, Issue 2
Written by Constantin CHIURCIU, Mădălina TABLICĂ, Lucica SIMA, Teodora SUPEANU, Mariana OPORANU
The present research focuses on the determination of the immunoglobulin Y (IgY) levels derived from egg yolks obtained from hens previously inoculated with a combination of bacterial and fungal antigens (multiple antigens). The purpose of the work consists of establishing the frequency of the inoculations, in order to obtain a high level of antibodies throughout the duration of the experiment. The antigens were obtained from inactivated bacterial and fungal humans pathogenic strains. Egg-laying hen lots were formed out of individuals at the beginning of the egg-laying period. The hens were inoculated with multiple antigens, three times, at the first, 14th and the 28th day of the experiment. The control for the immune response was performed by sampling eggs on the 14th day from the third inoculation process. The IgY was extracted from the egg yolk in order to obtain the aqueous phase. The characterization of the IgY titers was performed every 30 days, for a period of 9 months, by using qualitative and quantitative ELISA assays. Following the end of the 9 months period since the 3rd inoculation process, the specific IgY titers maintained at high levels, another (4th) inoculation shouldn’t be necessary.
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