Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIII, Issue 1
Written by Laurenţiu OGNEAN, Cristina ȘTEFĂNUŢ, Alina NĂSĂLEAN, Emöke PALL, Andreea BUTA, Attila HARI, Octavia NEGREA
The colostrum secretion of different mammalian species contains heterogeneous cellular populations which come from lactiferous structures and from the general circulation, including various types of epithelial cells, respectively leukocytes. During the synthetis and milk-ejection process, in lactation are involved different types of epithelial cells, with a wide morphological and functional diversity. The aim of this research is represented by the cytomorphological investigation of the first jennet colostrum, which was drawn from healthy mammary gland. The colostrum samples were processed in stained slides, using Dia Quick Panoptic method and they were examined at the microscope and they were evaluated by using the milk cytogram technique (Ognean et al., 2011). The conducted studies have highlighted morphological elements and activity states necessary for the characterization of the cellular populations, regarding the morpho-functional description of the epithelial cells. The results revealed a significant percentage of the epithelial cells in the jennet colostrum. In the configuration of this cellular population, we have identified alveolar, squamous and columnar epithelial cells. The alveolar cells have prevailed among the epithelial cells and they have reflected the secretory activity of the alveolar epithelium. The squamous epithelial cells were distinguished due to their predominant polygonal cytoplasm and the punctiform nucleous, while the columnar epithelial cells were elongate, ovalar or polygonal, with a porous structure. A special category was represented by the atypical cellular structures, that were polymorphous and sometimes mixed with cytoplasmatic particles and cellular debris. The results were analysed in the context of encouraging the use of the mammary epithelial cells in different domains of the scientific research. In conclusion, the high content of the epithelial cells in the jennet mammary secretions represent a disposable resource for the molecular study of the mammary gene expression, lactogenesis, immunity or mammary cancer.
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