Quality of fresh semen is a crucial element for the succes of assisted reproduction techniques in all species. 57 ejaculates were collected manually from 25 dogs. Motility and concentration (CASA SpermVision®, Minitübe, Germany, morphology and acrosome status (Spermac® stain) were determined. Semen analysis showed a strong relationship between male’s weight and size (testes dimensions) and the volume of the sperm rich fraction, but did not identify a correlation between concentration and male’s size. Motility parameters, morphology and acrosome status for the ejaculates included in our study were within the accepted standard values. Age did not significantly influence the volume or the morphology of canine semen.