Dogs have frequently infections with coagulase-positive and coagulase-negative staphylococci with different locations. A total of 49 samples consisting of pathological skin, auricular and genital secretions were bacteriologically examined using standard methodology. The isolates were tested by disk diffusion Kirby-Bauer method, on which were used biodiscs with 19 antibiotics from different groups. The staphylococci strains isolated from dogs included in Intermedius Group were susceptible to the antibiotics rarely or not used in the therapy of diseases in this species. The isolates were methicillin-resistant strains, thus emphasizing the movement of these strains in the canine population, confirming the zoonotic risk of these strains. After this study several resistant phenotypes of staphylococci strains included in Intermedius Group were identified, whose frequency was variable.