Published in Scientific Works. Series C. Veterinary Medicine, Vol. 19 ISSUE 3
Written by Anca Sofiana SURPAT, Diana BREZOVAN, Jelena SAVICI, Corina PASCU, Janos DEGI, Ovidiu MEDERLE, Viorel HERMAN
To compare different histopathological methods for diagnosis of Lawsonia intracellularis infection in pigs were taken in study 25 samples of ileum with specific lesions of intestinal adenomatosis. In order to perform slides were used Kinyoun, Green-Methyl-Pironine, Masson-Fontana, Schmitz, Diff-Quick methods and immunohistochemistry. The results showed that Green-Methyl-Pironine method has no value for diagnosis of porcine proliferative enteropathy, while Kinyoun coloration is capable to identify the bacteria only in 28% of samples. The argentic impregnation and Diff-Quick are able to highlight the aetiological agent in 44%, respectively 40% of the studied samples, so this methods have enlarge value of diagnosis. Immunohistochemistry demonstrated a high sensitivity and specificity and it was capable to emphasize the causative agent of intestinal adenomatosis in all 25 studied samples with proliferative ileitis.
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