Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXX, Issue 2
Written by Mădălina Iulia SITEAVU, Roxana Ionela DRUGEA, Elena PITOIU, Emilia CIOBOTARU-PÎRVU
Actinobacillus pleuropneumoniae is a bacterial porcine respiratory tract pathogen that causes porcine pleuropneumonia, with high economic consequences and distribution all over the world. This study aimed to assess the prevalence of A. pleuropneumoniae in Romanian swine farms by two methods of diagnosis: microbiological examination and Real-time PCR. Serotyping was performed on 28 bacterial isolates from 6 farms. From 1281 number of tested samples by microbiological examination, there were obtained 137 number of isolates with a positive result for A. pleuropneumoniae, with an overall prevalence of 11%. By Real-time PCR, 231 samples were tested and 100 (43%) were positive for A. pleuropneumoniae, 13/81 (16%) lung tissue samples, and 87/150 (58%) oral fluid samples. The serotyping of 28 A. pleuropneumoniae-positive cultures revealed the presence of the following serotypes: 1-9-11, 2, 3,
4, 5, 14, the most frequently encountered being serotype 2, in 10 isolates (36%) and serotype 14, in 7 isolates (25%).
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