Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 2
Written by Alina Elena ȘTEFAN, Daniela GOLOGAN, Sorin MUȘAT, Matthew Okerlund LEAVITT, Raluca STAN, Manuella MILITARU
The need of issuing medical diagnoses with fast turn-around times (but without compromising accuracy) is generating technical challenges in all stages of histological processing. Because of the multiple variables related to tissue harvesting, processing, and sectioning quite often the resulting histological slides reach the pathologist with fragmented or incomplete tissue sections. In the present study we evaluated the feasibility of a new method of multiplexing tissue specimens with irregular shapes by placing them during grossing into sectionable matrices (BxFrame™ GRID). The working time required for a histotechnologist in obtaining multiplexed preparations as well as the costs of laboratory supplies was compared with conventional methods. Five different types of tissue (duodenum, brain, heart, tail, and skin) were placed in BxFrame™ matrices, and subjected to histological processing, sectioned, and stained. The new multiplexing method reduced the total working time with 45% to 70% when compared to conventional methods (depending on the type of tissue) while the cost of consumables was reduced with up to 70%.
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