Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 2
Written by Nicolae Tiberiu CONSTANTIN, Florin Petrișor POSASTIUC, Crina Raluca ANDREI, Ovidiu GEICU, Florin IORDACHE, Loredana STANCA, Liviu BÎLTEANU, Andreea Iren ȘERBAN
Bovine herpesvirus-1(BoHV-1) infections can be asymptomatic nonetheless it can also cause systemic illness in young calves and several diseases in adult cattle, including infectious bovine rhinotracheitis, infectious pustular vulvovaginitis and abortions. BoHV-1 can also establish recurrent life-long latent infections after primary infection. For viral load detection and quantification, we analysed blood samples from 19 asymptomatic pregnant cows belonging to three different breeds (Montbéliard, Holstein and Romanian Black Spotted). Viral DNA extraction from plasma was performed using the Nucleic Acid Extraction or Purification Reagent Kit (Medicalsystem Biotechnology Co., Ltd. Ningbo, China), and the Auto-Pure 32A automatic extractor (Allsheng Instruments Co., Ltd. Hangzhou). The BHV-1 putative fibronectin binding protein Genesig Advanced Kit (Primerdesign Ltd, UK) was used for qPCR amplification. The qPCR reactions were performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA). After analysis of the amplification curves, only one sample out of 19 was positive with a viral load of 4×103 copies/mL of blood. The affected animal was asymptomatic, which emphasizes the need for recurrent testing of transmissible infectious pathogens, and increasing biovigilance for minimizing eventual economical losses.
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