Published in Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 1
Written by Fabiola IONIȚĂ, Cristin COMAN, Mario CODREANU
Inhalation anesthesia systems are used in laboratory animal experimentation due to their safety, easily adjustable dosage and rapid return to consciousness. Isoflurane is currently the most common volatile anesthetic used in mouse studies. The study aimed to investigate the influence of isoflurane on the vital functions during inhalation anesthesia in two mouse strains (inbred and outbred), suitable for blood collection and minor surgery. Heart rate, pulse distention, respiratory rate, peripheral arterial oxygen saturation and rectal temperature were measured during anesthesia and compared to the same parameters measured on awake animals. Results showed a decrease in the heart rate by 26% during 2% isoflurane anesthesia, while the breath rate decreased by 42%. Oxygen saturation remained at 95%–98% and the vascular distension caused by the pulse was relatively constant. Both groups showed a decrease in the rectal temperature by 1,6-2.2°C during anesthesia, with temperature values normalizing in 1.5-2 hours after anesthesia. The overall effects of isoflurane on mice vital signs were moderate, both induction and recovery from anesthesia proceeded quickly (1-4 minutes), with a rapid return of the animals to their normal state.
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